Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_697 and Pf1N1B4_698 are separated by 115 nucleotides Pf1N1B4_698 and Pf1N1B4_699 are separated by 155 nucleotides
Pf1N1B4_697 - Glycine cleavage system transcriptional activator, at 796,775 to 797,656
_697
Pf1N1B4_698 - Rrf2-linked NADH-flavin reductase, at 797,772 to 798,386
_698
Pf1N1B4_699 - Metallo-beta-lactamase superfamily protein PA0057, at 798,542 to 799,423
_699
Position (kb) 797
798
799 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 796.919 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.204 kb on + strand, within Pf1N1B4_697 at 797.205 kb on - strand, within Pf1N1B4_697 at 797.567 kb on + strand, within Pf1N1B4_697 at 797.570 kb on + strand at 797.787 kb on - strand at 797.804 kb on - strand at 797.804 kb on - strand at 797.804 kb on - strand at 797.855 kb on + strand, within Pf1N1B4_698 at 797.856 kb on - strand, within Pf1N1B4_698 at 797.856 kb on - strand, within Pf1N1B4_698 at 797.912 kb on + strand, within Pf1N1B4_698 at 797.990 kb on + strand, within Pf1N1B4_698 at 797.991 kb on - strand, within Pf1N1B4_698 at 797.991 kb on - strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.028 kb on + strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698 at 798.029 kb on - strand, within Pf1N1B4_698 at 798.687 kb on + strand at 798.687 kb on + strand, within Pf1N1B4_699 at 798.687 kb on + strand, within Pf1N1B4_699 at 798.688 kb on - strand at 798.688 kb on - strand, within Pf1N1B4_699 at 798.688 kb on - strand, within Pf1N1B4_699 at 798.688 kb on - strand, within Pf1N1B4_699 at 798.739 kb on - strand, within Pf1N1B4_699 at 798.739 kb on - strand, within Pf1N1B4_699 at 798.739 kb on - strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.143 kb on + strand, within Pf1N1B4_699 at 799.144 kb on - strand, within Pf1N1B4_699 at 799.144 kb on - strand, within Pf1N1B4_699 at 799.144 kb on - strand, within Pf1N1B4_699 at 799.146 kb on + strand, within Pf1N1B4_699 at 799.147 kb on - strand, within Pf1N1B4_699 at 799.147 kb on - strand, within Pf1N1B4_699 at 799.147 kb on - strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.170 kb on + strand, within Pf1N1B4_699 at 799.171 kb on - strand, within Pf1N1B4_699 at 799.171 kb on - strand, within Pf1N1B4_699 at 799.171 kb on - strand, within Pf1N1B4_699 at 799.323 kb on + strand, within Pf1N1B4_699
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 796,919 + Pf1N1B4_697 0.16 -1.0 797,204 + Pf1N1B4_697 0.49 +0.2 797,204 + Pf1N1B4_697 0.49 +0.0 797,204 + Pf1N1B4_697 0.49 -1.2 797,204 + Pf1N1B4_697 0.49 +0.3 797,204 + Pf1N1B4_697 0.49 +0.6 797,204 + Pf1N1B4_697 0.49 -2.2 797,204 + Pf1N1B4_697 0.49 +0.0 797,204 + Pf1N1B4_697 0.49 -0.7 797,204 + Pf1N1B4_697 0.49 +1.2 797,204 + Pf1N1B4_697 0.49 -1.4 797,205 - Pf1N1B4_697 0.49 -0.2 797,567 + Pf1N1B4_697 0.90 -1.6 797,570 + -0.6 797,787 - +2.2 797,804 - -1.6 797,804 - -1.2 797,804 - +0.8 797,855 + Pf1N1B4_698 0.13 -1.5 797,856 - Pf1N1B4_698 0.14 -2.1 797,856 - Pf1N1B4_698 0.14 -0.8 797,912 + Pf1N1B4_698 0.23 -0.2 797,990 + Pf1N1B4_698 0.35 -2.6 797,991 - Pf1N1B4_698 0.36 -3.0 797,991 - Pf1N1B4_698 0.36 +0.8 798,028 + Pf1N1B4_698 0.42 -0.6 798,028 + Pf1N1B4_698 0.42 +0.8 798,028 + Pf1N1B4_698 0.42 -3.4 798,028 + Pf1N1B4_698 0.42 -0.8 798,029 - Pf1N1B4_698 0.42 -2.0 798,029 - Pf1N1B4_698 0.42 +0.8 798,029 - Pf1N1B4_698 0.42 -1.6 798,687 + -2.0 798,687 + Pf1N1B4_699 0.16 -2.9 798,687 + Pf1N1B4_699 0.16 +0.8 798,688 - -3.0 798,688 - Pf1N1B4_699 0.17 -2.7 798,688 - Pf1N1B4_699 0.17 +0.2 798,688 - Pf1N1B4_699 0.17 -3.8 798,739 - Pf1N1B4_699 0.22 +0.8 798,739 - Pf1N1B4_699 0.22 -2.0 798,739 - Pf1N1B4_699 0.22 -0.8 799,143 + Pf1N1B4_699 0.68 -2.0 799,143 + Pf1N1B4_699 0.68 -0.4 799,143 + Pf1N1B4_699 0.68 +0.8 799,143 + Pf1N1B4_699 0.68 -2.8 799,143 + Pf1N1B4_699 0.68 +1.0 799,143 + Pf1N1B4_699 0.68 -2.0 799,143 + Pf1N1B4_699 0.68 +1.4 799,144 - Pf1N1B4_699 0.68 -1.6 799,144 - Pf1N1B4_699 0.68 -1.4 799,144 - Pf1N1B4_699 0.68 +0.8 799,146 + Pf1N1B4_699 0.68 -0.8 799,147 - Pf1N1B4_699 0.69 +0.1 799,147 - Pf1N1B4_699 0.69 -0.6 799,147 - Pf1N1B4_699 0.69 +0.2 799,170 + Pf1N1B4_699 0.71 +0.8 799,170 + Pf1N1B4_699 0.71 -2.4 799,170 + Pf1N1B4_699 0.71 +0.8 799,170 + -0.7 799,170 + Pf1N1B4_699 0.71 +0.8 799,170 + Pf1N1B4_699 0.71 -3.9 799,170 + Pf1N1B4_699 0.71 -1.0 799,170 + Pf1N1B4_699 0.71 +0.5 799,171 - Pf1N1B4_699 0.71 +0.2 799,171 - Pf1N1B4_699 0.71 -0.8 799,171 - Pf1N1B4_699 0.71 +0.8 799,323 + Pf1N1B4_699 0.89 -0.2
Or see this region's nucleotide sequence