Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6007

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_6006 and Pf1N1B4_6007 are separated by 412 nucleotidesPf1N1B4_6007 and Pf1N1B4_6008 are separated by 348 nucleotides Pf1N1B4_6006 - tgtA5 cluster protein 1, at 461,748 to 462,803 _6006 Pf1N1B4_6007 - Queuosine Biosynthesis QueC ATPase, at 463,216 to 463,533 _6007 Pf1N1B4_6008 - Queuosine Biosynthesis QueE Radical SAM, at 463,882 to 464,232 _6008 Position (kb) 463 464Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 462.597 kb on + strand, within Pf1N1B4_6006at 462.762 kb on + strandat 462.762 kb on + strandat 462.762 kb on + strandat 462.762 kb on + strandat 462.849 kb on - strandat 462.849 kb on - strandat 462.937 kb on + strandat 462.992 kb on + strandat 462.992 kb on + strandat 462.992 kb on + strandat 462.996 kb on + strandat 462.996 kb on + strandat 463.040 kb on + strandat 463.040 kb on + strandat 463.040 kb on + strandat 463.100 kb on + strandat 463.128 kb on + strandat 463.128 kb on + strandat 463.128 kb on + strandat 463.129 kb on - strandat 463.373 kb on + strand, within Pf1N1B4_6007at 463.373 kb on + strand, within Pf1N1B4_6007at 463.373 kb on + strand, within Pf1N1B4_6007at 463.374 kb on - strand, within Pf1N1B4_6007at 463.374 kb on - strand, within Pf1N1B4_6007at 463.374 kb on - strand, within Pf1N1B4_6007at 463.378 kb on - strandat 463.513 kb on + strandat 463.513 kb on + strandat 463.513 kb on + strandat 463.514 kb on - strandat 463.589 kb on + strandat 463.590 kb on - strandat 463.590 kb on - strandat 463.625 kb on - strandat 463.689 kb on + strandat 463.689 kb on + strandat 463.690 kb on - strandat 463.690 kb on - strandat 463.690 kb on - strandat 463.734 kb on + strandat 463.734 kb on + strandat 463.735 kb on - strandat 463.815 kb on + strandat 463.847 kb on - strandat 463.849 kb on - strandat 463.852 kb on - strandat 463.995 kb on - strand, within Pf1N1B4_6008at 464.241 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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462,597 + Pf1N1B4_6006 0.80 -1.8
462,762 + +0.4
462,762 + -0.0
462,762 + +1.0
462,762 + +0.7
462,849 - -2.3
462,849 - -0.8
462,937 + -0.6
462,992 + +1.7
462,992 + -1.9
462,992 + -1.7
462,996 + -2.7
462,996 + -2.3
463,040 + -1.5
463,040 + -0.9
463,040 + +0.7
463,100 + +0.7
463,128 + -0.1
463,128 + -1.3
463,128 + -1.5
463,129 - +0.7
463,373 + Pf1N1B4_6007 0.49 -1.1
463,373 + Pf1N1B4_6007 0.49 +0.3
463,373 + Pf1N1B4_6007 0.49 +0.7
463,374 - Pf1N1B4_6007 0.50 -0.3
463,374 - Pf1N1B4_6007 0.50 +0.7
463,374 - Pf1N1B4_6007 0.50 -0.4
463,378 - -0.3
463,513 + +2.2
463,513 + +0.7
463,513 + +0.7
463,514 - -0.5
463,589 + -1.4
463,590 - +0.7
463,590 - -0.3
463,625 - +0.7
463,689 + -2.8
463,689 + +1.9
463,690 - +0.2
463,690 - -3.3
463,690 - -2.1
463,734 + -1.1
463,734 + +0.3
463,735 - +0.7
463,815 + -0.9
463,847 - +0.7
463,849 - +0.2
463,852 - -0.3
463,995 - Pf1N1B4_6008 0.32 +0.7
464,241 + +0.7

Or see this region's nucleotide sequence