Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5857 and Pf1N1B4_5858 are separated by 340 nucleotides Pf1N1B4_5858 and Pf1N1B4_5859 are separated by 229 nucleotides
Pf1N1B4_5857 - Arginine N-succinyltransferase (EC 2.3.1.109), at 289,942 to 290,976
_5857
Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186
_5858
Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657
_5859
Position (kb) 291
292
293 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 290.340 kb on + strand, within Pf1N1B4_5857 at 290.681 kb on - strand, within Pf1N1B4_5857 at 291.153 kb on - strand at 291.153 kb on - strand at 291.247 kb on - strand at 291.302 kb on - strand at 291.317 kb on - strand at 291.350 kb on - strand at 291.383 kb on + strand at 291.931 kb on - strand, within Pf1N1B4_5858 at 291.950 kb on + strand, within Pf1N1B4_5858 at 291.951 kb on - strand, within Pf1N1B4_5858 at 292.028 kb on + strand, within Pf1N1B4_5858 at 292.029 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.128 kb on - strand at 292.128 kb on - strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.167 kb on - strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.268 kb on + strand at 292.268 kb on + strand at 292.269 kb on - strand at 292.269 kb on - strand at 292.457 kb on - strand at 292.503 kb on - strand at 292.503 kb on - strand at 292.508 kb on + strand at 292.509 kb on - strand at 292.695 kb on - strand, within Pf1N1B4_5859 at 292.701 kb on - strand at 293.067 kb on + strand, within Pf1N1B4_5859 at 293.068 kb on - strand, within Pf1N1B4_5859
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 290,340 + Pf1N1B4_5857 0.38 -2.3 290,681 - Pf1N1B4_5857 0.71 +0.2 291,153 - -2.8 291,153 - -2.7 291,247 - +0.9 291,302 - -2.8 291,317 - -1.4 291,350 - -0.9 291,383 + -0.1 291,931 - Pf1N1B4_5858 0.71 -3.2 291,950 + Pf1N1B4_5858 0.73 -1.9 291,951 - Pf1N1B4_5858 0.73 -1.8 292,028 + Pf1N1B4_5858 0.82 -1.6 292,029 - Pf1N1B4_5858 0.82 -2.7 292,053 - Pf1N1B4_5858 0.85 +0.7 292,053 - Pf1N1B4_5858 0.85 -2.7 292,123 + +1.1 292,123 + -3.1 292,123 + -1.7 292,123 + +1.9 292,123 + -2.3 292,124 - +0.7 292,124 - +0.7 292,124 - +0.7 292,124 - -2.5 292,124 - -1.3 292,124 - +0.7 292,124 - +0.7 292,127 + +1.3 292,127 + +1.2 292,127 + +0.7 292,128 - -2.3 292,128 - -1.0 292,145 + -0.9 292,145 + -0.7 292,145 + +0.7 292,145 + -1.7 292,146 - +1.1 292,146 - +0.7 292,146 - -2.3 292,146 - +0.6 292,166 + -0.2 292,166 + -1.1 292,166 + -0.2 292,166 + -0.5 292,166 + +0.7 292,167 - -1.1 292,168 + -0.3 292,168 + +2.3 292,168 + -2.7 292,168 + -4.0 292,169 - -0.5 292,169 - -1.3 292,169 - -2.3 292,169 - -2.5 292,169 - -0.1 292,268 + +0.7 292,268 + +1.0 292,269 - -0.8 292,269 - -0.2 292,457 - -1.1 292,503 - +0.7 292,503 - +0.7 292,508 + -2.3 292,509 - +0.1 292,695 - Pf1N1B4_5859 0.22 -1.7 292,701 - +0.7 293,067 + Pf1N1B4_5859 0.52 +0.2 293,068 - Pf1N1B4_5859 0.52 +0.8
Or see this region's nucleotide sequence