Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides Pf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotides Pf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotides Pf1N1B4_5851 and Pf1N1B4_5852 are separated by 2 nucleotides Pf1N1B4_5852 and Pf1N1B4_5853 overlap by 4 nucleotides
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509
_5850
Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611
_5851
Pf1N1B4_5852 - Amidases related to nicotinamidase, at 284,614 to 285,258
_5852
Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586
_5853
Position (kb)
283
284
285 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 282.888 kb on + strand at 282.889 kb on - strand at 283.020 kb on - strand at 283.020 kb on - strand, within Pf1N1B4_5849 at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.683 kb on + strand at 283.683 kb on + strand at 283.801 kb on + strand at 283.824 kb on + strand at 283.825 kb on - strand at 284.036 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.058 kb on - strand, within Pf1N1B4_5851 at 284.118 kb on + strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.119 kb on - strand, within Pf1N1B4_5851 at 284.402 kb on + strand, within Pf1N1B4_5851 at 284.403 kb on - strand at 284.609 kb on + strand at 284.610 kb on - strand at 285.154 kb on + strand, within Pf1N1B4_5852 at 285.155 kb on - strand at 285.172 kb on + strand, within Pf1N1B4_5852 at 285.444 kb on + strand, within Pf1N1B4_5853
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 282,888 + -0.7 282,889 - -1.7 283,020 - +0.7 283,020 - Pf1N1B4_5849 0.35 +0.7 283,530 + -1.1 283,530 + -0.5 283,531 - -1.5 283,531 - -2.7 283,683 + -1.2 283,683 + +0.0 283,801 + +0.7 283,824 + -0.6 283,825 - -0.5 284,036 - Pf1N1B4_5851 0.33 -1.9 284,058 - Pf1N1B4_5851 0.35 -1.7 284,058 - Pf1N1B4_5851 0.35 -1.3 284,118 + Pf1N1B4_5851 0.42 -2.0 284,119 - Pf1N1B4_5851 0.42 -0.5 284,119 - Pf1N1B4_5851 0.42 +0.7 284,402 + Pf1N1B4_5851 0.75 +0.7 284,403 - -3.5 284,609 + +0.5 284,610 - -0.7 285,154 + Pf1N1B4_5852 0.84 -1.5 285,155 - +0.7 285,172 + Pf1N1B4_5852 0.87 -0.2 285,444 + Pf1N1B4_5853 0.14 -1.0
Or see this region's nucleotide sequence