Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5851

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotidesPf1N1B4_5849 and Pf1N1B4_5850 overlap by 50 nucleotidesPf1N1B4_5850 and Pf1N1B4_5851 are separated by 247 nucleotidesPf1N1B4_5851 and Pf1N1B4_5852 are separated by 2 nucleotidesPf1N1B4_5852 and Pf1N1B4_5853 overlap by 4 nucleotides Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852 _5848 Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337 _5849 Pf1N1B4_5850 - hypothetical protein, at 283,288 to 283,509 _5850 Pf1N1B4_5851 - Transcriptional regulator, RpiR family, at 283,757 to 284,611 _5851 Pf1N1B4_5852 - Amidases related to nicotinamidase, at 284,614 to 285,258 _5852 Pf1N1B4_5853 - Glutamine synthetase (EC 6.3.1.2), putative, at 285,255 to 286,586 _5853 Position (kb) 283 284 285Strain fitness (log2 ratio) -3 -2 -1 0 1at 282.888 kb on + strandat 282.889 kb on - strandat 283.020 kb on - strandat 283.020 kb on - strand, within Pf1N1B4_5849at 283.530 kb on + strandat 283.530 kb on + strandat 283.531 kb on - strandat 283.531 kb on - strandat 283.683 kb on + strandat 283.683 kb on + strandat 283.801 kb on + strandat 283.824 kb on + strandat 283.825 kb on - strandat 284.036 kb on - strand, within Pf1N1B4_5851at 284.058 kb on - strand, within Pf1N1B4_5851at 284.058 kb on - strand, within Pf1N1B4_5851at 284.118 kb on + strand, within Pf1N1B4_5851at 284.119 kb on - strand, within Pf1N1B4_5851at 284.119 kb on - strand, within Pf1N1B4_5851at 284.402 kb on + strand, within Pf1N1B4_5851at 284.403 kb on - strandat 284.609 kb on + strandat 284.610 kb on - strandat 285.154 kb on + strand, within Pf1N1B4_5852at 285.155 kb on - strandat 285.172 kb on + strand, within Pf1N1B4_5852at 285.444 kb on + strand, within Pf1N1B4_5853

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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282,888 + -0.7
282,889 - -1.7
283,020 - +0.7
283,020 - Pf1N1B4_5849 0.35 +0.7
283,530 + -1.1
283,530 + -0.5
283,531 - -1.5
283,531 - -2.7
283,683 + -1.2
283,683 + +0.0
283,801 + +0.7
283,824 + -0.6
283,825 - -0.5
284,036 - Pf1N1B4_5851 0.33 -1.9
284,058 - Pf1N1B4_5851 0.35 -1.7
284,058 - Pf1N1B4_5851 0.35 -1.3
284,118 + Pf1N1B4_5851 0.42 -2.0
284,119 - Pf1N1B4_5851 0.42 -0.5
284,119 - Pf1N1B4_5851 0.42 +0.7
284,402 + Pf1N1B4_5851 0.75 +0.7
284,403 - -3.5
284,609 + +0.5
284,610 - -0.7
285,154 + Pf1N1B4_5852 0.84 -1.5
285,155 - +0.7
285,172 + Pf1N1B4_5852 0.87 -0.2
285,444 + Pf1N1B4_5853 0.14 -1.0

Or see this region's nucleotide sequence