Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5845 and Pf1N1B4_5846 are separated by 117 nucleotides Pf1N1B4_5846 and Pf1N1B4_5847 are separated by 101 nucleotides
Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321
_5845
Pf1N1B4_5846 - Rhodanese-related sulfurtransferase, at 280,439 to 280,873
_5846
Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949
_5847
Position (kb) 280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 279.483 kb on + strand at 279.483 kb on + strand at 279.484 kb on - strand at 279.484 kb on - strand at 279.487 kb on + strand at 279.489 kb on + strand at 279.516 kb on - strand at 279.840 kb on - strand, within Pf1N1B4_5845 at 280.369 kb on - strand at 280.369 kb on - strand at 280.369 kb on - strand at 280.403 kb on + strand at 280.404 kb on - strand at 280.612 kb on - strand, within Pf1N1B4_5846 at 280.774 kb on - strand, within Pf1N1B4_5846 at 280.880 kb on + strand at 280.880 kb on + strand at 280.881 kb on - strand at 280.914 kb on - strand at 280.914 kb on - strand at 281.011 kb on + strand at 281.012 kb on - strand at 281.197 kb on - strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.425 kb on + strand at 281.425 kb on + strand, within Pf1N1B4_5847
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 279,483 + +0.7 279,483 + -1.6 279,484 - -0.5 279,484 - +0.7 279,487 + -0.9 279,489 + +0.4 279,516 - +0.7 279,840 - Pf1N1B4_5845 0.25 -3.4 280,369 - -1.7 280,369 - -1.3 280,369 - +0.2 280,403 + +0.7 280,404 - -0.3 280,612 - Pf1N1B4_5846 0.40 -0.7 280,774 - Pf1N1B4_5846 0.77 +1.0 280,880 + +0.7 280,880 + -1.9 280,881 - +0.7 280,914 - -1.7 280,914 - +0.1 281,011 + +0.2 281,012 - -1.4 281,197 - Pf1N1B4_5847 0.23 -0.1 281,402 + Pf1N1B4_5847 0.44 -2.5 281,402 + Pf1N1B4_5847 0.44 -1.3 281,402 + Pf1N1B4_5847 0.44 +0.7 281,402 + Pf1N1B4_5847 0.44 -0.7 281,402 + Pf1N1B4_5847 0.44 -0.8 281,403 - Pf1N1B4_5847 0.44 +0.7 281,403 - Pf1N1B4_5847 0.44 -2.3 281,403 - Pf1N1B4_5847 0.44 -3.7 281,403 - -1.7 281,425 + Pf1N1B4_5847 0.46 -1.3 281,425 + +0.7 281,425 + Pf1N1B4_5847 0.46 +1.2
Or see this region's nucleotide sequence