Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotides Pf1N1B4_5834 and Pf1N1B4_5835 are separated by 11 nucleotides Pf1N1B4_5835 and Pf1N1B4_5836 are separated by 133 nucleotides Pf1N1B4_5836 and Pf1N1B4_5837 are separated by 110 nucleotides
Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543
_5833
Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245
_5834
Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438
_5835
Pf1N1B4_5836 - Putative polyketide synthase (Fragment), at 270,572 to 271,174
_5836
Pf1N1B4_5837 - Transcriptional regulator, LysR family, at 271,285 to 272,181
_5837
Position (kb) 269
270
271 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 268.275 kb on - strand, within Pf1N1B4_5833 at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.479 kb on - strand at 268.479 kb on - strand at 268.572 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.641 kb on - strand at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 269.423 kb on + strand, within Pf1N1B4_5835 at 269.623 kb on + strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.758 kb on + strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835 at 270.436 kb on + strand at 270.553 kb on + strand at 270.554 kb on - strand at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.050 kb on - strand, within Pf1N1B4_5836 at 271.182 kb on - strand at 271.209 kb on - strand at 271.267 kb on + strand at 271.267 kb on + strand at 271.270 kb on + strand at 271.271 kb on - strand at 271.428 kb on - strand, within Pf1N1B4_5837
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 268,275 - Pf1N1B4_5833 0.62 -1.9 268,478 + +0.8 268,478 + +0.7 268,478 + +0.3 268,478 + +0.3 268,479 - +0.7 268,479 - +0.7 268,572 + +0.7 268,640 + +0.7 268,640 + -0.4 268,640 + -1.1 268,641 - +0.7 268,682 + Pf1N1B4_5834 0.15 -2.9 268,682 + Pf1N1B4_5834 0.15 -1.2 268,683 - Pf1N1B4_5834 0.15 -0.9 268,683 - Pf1N1B4_5834 0.15 +0.3 268,683 - Pf1N1B4_5834 0.15 +0.7 269,423 + Pf1N1B4_5835 0.14 +0.3 269,623 + Pf1N1B4_5835 0.31 -0.2 269,624 - Pf1N1B4_5835 0.31 +0.7 269,624 - Pf1N1B4_5835 0.31 -1.0 269,624 - Pf1N1B4_5835 0.31 -0.7 269,758 + Pf1N1B4_5835 0.42 +0.7 269,759 - Pf1N1B4_5835 0.42 +1.2 269,759 - Pf1N1B4_5835 0.42 +0.7 270,436 + -0.1 270,553 + -1.0 270,554 - -0.9 271,049 + Pf1N1B4_5836 0.79 +0.5 271,049 + Pf1N1B4_5836 0.79 +0.7 271,050 - Pf1N1B4_5836 0.79 -0.7 271,182 - -0.6 271,209 - +0.7 271,267 + -0.0 271,267 + -1.4 271,270 + +0.7 271,271 - -0.2 271,428 - Pf1N1B4_5837 0.16 -0.1
Or see this region's nucleotide sequence