Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5732 and Pf1N1B4_5733 overlap by 8 nucleotides Pf1N1B4_5733 and Pf1N1B4_5734 are separated by 2 nucleotides Pf1N1B4_5734 and Pf1N1B4_5735 are separated by 1 nucleotides
Pf1N1B4_5732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 171,856 to 172,536
_5732
Pf1N1B4_5733 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 172,529 to 173,284
_5733
Pf1N1B4_5734 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 173,287 to 174,825
_5734
Pf1N1B4_5735 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 174,827 to 178,600
_5735
Position (kb) 173
174
175 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 172.502 kb on + strand at 173.474 kb on + strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.475 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand, within Pf1N1B4_5734 at 173.499 kb on - strand at 173.499 kb on - strand, within Pf1N1B4_5734 at 173.663 kb on + strand, within Pf1N1B4_5734 at 173.663 kb on + strand, within Pf1N1B4_5734 at 173.664 kb on - strand, within Pf1N1B4_5734 at 173.664 kb on - strand, within Pf1N1B4_5734 at 173.838 kb on - strand, within Pf1N1B4_5734 at 174.132 kb on - strand, within Pf1N1B4_5734 at 174.224 kb on + strand, within Pf1N1B4_5734 at 174.293 kb on + strand, within Pf1N1B4_5734 at 174.293 kb on + strand at 174.293 kb on + strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.294 kb on - strand, within Pf1N1B4_5734 at 174.525 kb on - strand, within Pf1N1B4_5734 at 174.528 kb on - strand, within Pf1N1B4_5734 at 174.714 kb on - strand at 174.955 kb on - strand at 174.957 kb on + strand at 174.958 kb on - strand at 174.958 kb on - strand at 174.958 kb on - strand at 175.101 kb on + strand at 175.102 kb on - strand at 175.284 kb on + strand, within Pf1N1B4_5735 at 175.285 kb on - strand, within Pf1N1B4_5735 at 175.522 kb on - strand, within Pf1N1B4_5735
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 172,502 + -2.4 173,474 + Pf1N1B4_5734 0.12 +0.6 173,475 - Pf1N1B4_5734 0.12 +0.6 173,475 - Pf1N1B4_5734 0.12 -1.7 173,499 - Pf1N1B4_5734 0.14 +0.6 173,499 - +0.6 173,499 - Pf1N1B4_5734 0.14 -2.0 173,663 + Pf1N1B4_5734 0.24 -1.7 173,663 + Pf1N1B4_5734 0.24 -2.7 173,664 - Pf1N1B4_5734 0.24 -2.8 173,664 - Pf1N1B4_5734 0.24 -0.4 173,838 - Pf1N1B4_5734 0.36 +0.6 174,132 - Pf1N1B4_5734 0.55 -3.2 174,224 + Pf1N1B4_5734 0.61 -2.4 174,293 + Pf1N1B4_5734 0.65 +0.6 174,293 + -1.0 174,293 + Pf1N1B4_5734 0.65 -1.7 174,294 - Pf1N1B4_5734 0.65 +0.6 174,294 - Pf1N1B4_5734 0.65 +0.6 174,294 - Pf1N1B4_5734 0.65 -1.4 174,525 - Pf1N1B4_5734 0.80 -2.7 174,528 - Pf1N1B4_5734 0.81 -4.0 174,714 - -1.7 174,955 - +0.6 174,957 + -2.1 174,958 - -1.4 174,958 - +0.6 174,958 - -2.7 175,101 + -3.3 175,102 - -2.8 175,284 + Pf1N1B4_5735 0.12 -3.9 175,285 - Pf1N1B4_5735 0.12 -1.9 175,522 - Pf1N1B4_5735 0.18 -2.2
Or see this region's nucleotide sequence