Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides Pf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1 at 163.936 kb on - strand, within Pf1N1B4_5722 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724 at 165.101 kb on - strand, within Pf1N1B4_5725 at 165.385 kb on + strand at 165.394 kb on - strand at 165.485 kb on - strand, within Pf1N1B4_5726 at 165.594 kb on + strand, within Pf1N1B4_5726 at 165.699 kb on + strand, within Pf1N1B4_5726 at 166.054 kb on + strand, within Pf1N1B4_5726
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 163,936 - Pf1N1B4_5722 0.59 +0.0 164,798 + Pf1N1B4_5724 0.70 +1.3 164,798 + Pf1N1B4_5724 0.70 -2.6 164,798 + Pf1N1B4_5724 0.70 -2.5 164,801 + Pf1N1B4_5724 0.71 -0.4 164,802 - Pf1N1B4_5724 0.71 +0.5 165,101 - Pf1N1B4_5725 0.47 -2.0 165,385 + -0.6 165,394 - -1.0 165,485 - Pf1N1B4_5726 0.11 -1.8 165,594 + Pf1N1B4_5726 0.19 +1.0 165,699 + Pf1N1B4_5726 0.27 -1.7 166,054 + Pf1N1B4_5726 0.54 -2.1
Or see this region's nucleotide sequence