Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5710

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5709 and Pf1N1B4_5710 are separated by 22 nucleotidesPf1N1B4_5710 and Pf1N1B4_5711 are separated by 92 nucleotides Pf1N1B4_5709 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 147,817 to 149,052 _5709 Pf1N1B4_5710 - Transcriptional regulator, TetR family, at 149,075 to 149,629 _5710 Pf1N1B4_5711 - Salicylate hydroxylase (EC 1.14.13.1), at 149,722 to 151,380 _5711 Position (kb) 149 150Strain fitness (log2 ratio) -2 -1 0 1at 148.148 kb on + strand, within Pf1N1B4_5709at 148.148 kb on + strand, within Pf1N1B4_5709at 148.149 kb on - strand, within Pf1N1B4_5709at 148.278 kb on + strand, within Pf1N1B4_5709at 148.604 kb on - strand, within Pf1N1B4_5709at 148.664 kb on - strand, within Pf1N1B4_5709at 149.026 kb on + strandat 149.121 kb on - strandat 149.175 kb on - strand, within Pf1N1B4_5710at 149.219 kb on + strand, within Pf1N1B4_5710at 149.219 kb on + strand, within Pf1N1B4_5710at 149.526 kb on + strand, within Pf1N1B4_5710at 149.527 kb on - strand, within Pf1N1B4_5710at 149.732 kb on - strandat 149.856 kb on + strandat 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.385 kb on + strand, within Pf1N1B4_5711at 150.489 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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148,148 + Pf1N1B4_5709 0.27 -2.7
148,148 + Pf1N1B4_5709 0.27 +1.1
148,149 - Pf1N1B4_5709 0.27 -1.3
148,278 + Pf1N1B4_5709 0.37 +0.6
148,604 - Pf1N1B4_5709 0.64 +0.6
148,664 - Pf1N1B4_5709 0.69 -1.3
149,026 + +0.6
149,121 - +0.6
149,175 - Pf1N1B4_5710 0.18 +0.6
149,219 + Pf1N1B4_5710 0.26 -2.4
149,219 + Pf1N1B4_5710 0.26 -0.8
149,526 + Pf1N1B4_5710 0.81 +1.0
149,527 - Pf1N1B4_5710 0.81 +0.2
149,732 - +0.6
149,856 + +1.2
150,374 + Pf1N1B4_5711 0.39 -2.6
150,374 + Pf1N1B4_5711 0.39 -1.0
150,374 + Pf1N1B4_5711 0.39 +1.6
150,374 + Pf1N1B4_5711 0.39 +0.6
150,385 + Pf1N1B4_5711 0.40 +0.6
150,489 + -2.5

Or see this region's nucleotide sequence