Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5650 and Pf1N1B4_5651 are separated by 184 nucleotides Pf1N1B4_5651 and Pf1N1B4_5652 are separated by 119 nucleotides Pf1N1B4_5652 and Pf1N1B4_5653 are separated by 149 nucleotides
Pf1N1B4_5650 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 77,942 to 79,588
_5650
Pf1N1B4_5651 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 79,773 to 80,318
_5651
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Pf1N1B4_5653 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 81,598 to 85,464
_5653
Position (kb) 80
81
82 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 79.539 kb on + strand at 79.540 kb on - strand at 79.797 kb on - strand at 80.247 kb on + strand, within Pf1N1B4_5651 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.171 kb on - strand, within Pf1N1B4_5652 at 81.614 kb on - strand at 81.614 kb on - strand at 81.768 kb on - strand at 81.768 kb on - strand at 81.870 kb on + strand at 81.870 kb on + strand at 81.871 kb on - strand at 81.871 kb on - strand at 82.058 kb on + strand, within Pf1N1B4_5653
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 79,539 + +0.2 79,540 - +0.6 79,797 - +0.6 80,247 + Pf1N1B4_5651 0.87 -0.6 81,141 + Pf1N1B4_5652 0.70 +0.6 81,141 + Pf1N1B4_5652 0.70 -0.2 81,142 - Pf1N1B4_5652 0.70 -1.7 81,142 - Pf1N1B4_5652 0.70 -2.5 81,142 - Pf1N1B4_5652 0.70 -1.7 81,142 - Pf1N1B4_5652 0.70 -3.8 81,142 - Pf1N1B4_5652 0.70 -1.6 81,171 - Pf1N1B4_5652 0.73 -3.0 81,614 - -1.7 81,614 - -2.8 81,768 - -0.6 81,768 - +0.4 81,870 + +1.6 81,870 + -3.7 81,871 - -3.2 81,871 - -1.0 82,058 + Pf1N1B4_5653 0.12 +0.0
Or see this region's nucleotide sequence