Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5586 and Pf1N1B4_5587 overlap by 4 nucleotides Pf1N1B4_5587 and Pf1N1B4_5588 are separated by 10 nucleotides Pf1N1B4_5588 and Pf1N1B4_5589 are separated by 83 nucleotides
Pf1N1B4_5586 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 7,744 to 8,790
_5586
Pf1N1B4_5587 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 8,787 to 9,737
_5587
Pf1N1B4_5588 - Transcriptional regulator for ferulate or vanillate catabolism, at 9,748 to 10,461
_5588
Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786
_5589
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.265 kb on + strand, within Pf1N1B4_5587 at 9.266 kb on - strand at 9.266 kb on - strand at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.346 kb on + strand, within Pf1N1B4_5587 at 9.347 kb on - strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.348 kb on + strand, within Pf1N1B4_5587 at 9.349 kb on - strand at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.349 kb on - strand, within Pf1N1B4_5587 at 9.547 kb on + strand, within Pf1N1B4_5587 at 9.548 kb on - strand, within Pf1N1B4_5587 at 9.739 kb on - strand at 9.750 kb on - strand at 9.914 kb on + strand, within Pf1N1B4_5588 at 9.915 kb on - strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.094 kb on - strand, within Pf1N1B4_5588 at 10.456 kb on - strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.493 kb on - strand at 10.506 kb on - strand at 10.506 kb on - strand at 11.111 kb on + strand, within Pf1N1B4_5589 at 11.112 kb on - strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.314 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 9,265 + Pf1N1B4_5587 0.50 -3.0 9,265 + Pf1N1B4_5587 0.50 -0.0 9,265 + Pf1N1B4_5587 0.50 -0.5 9,266 - +1.3 9,266 - -2.2 9,346 + Pf1N1B4_5587 0.59 -1.1 9,346 + Pf1N1B4_5587 0.59 -3.4 9,346 + Pf1N1B4_5587 0.59 -1.1 9,346 + Pf1N1B4_5587 0.59 -1.0 9,346 + Pf1N1B4_5587 0.59 -2.5 9,347 - Pf1N1B4_5587 0.59 +0.6 9,348 + Pf1N1B4_5587 0.59 -1.6 9,348 + Pf1N1B4_5587 0.59 +1.7 9,348 + Pf1N1B4_5587 0.59 -1.0 9,348 + Pf1N1B4_5587 0.59 +0.0 9,348 + Pf1N1B4_5587 0.59 -2.6 9,349 - +2.0 9,349 - Pf1N1B4_5587 0.59 +0.8 9,349 - Pf1N1B4_5587 0.59 -0.6 9,349 - Pf1N1B4_5587 0.59 -1.0 9,349 - Pf1N1B4_5587 0.59 -2.4 9,547 + Pf1N1B4_5587 0.80 -0.2 9,548 - Pf1N1B4_5587 0.80 -1.0 9,739 - -0.7 9,750 - -3.5 9,914 + Pf1N1B4_5588 0.23 -1.3 9,915 - Pf1N1B4_5588 0.23 -1.7 10,093 + Pf1N1B4_5588 0.48 -2.0 10,093 + Pf1N1B4_5588 0.48 -2.5 10,094 - Pf1N1B4_5588 0.48 -0.4 10,456 - -1.1 10,492 + -0.7 10,492 + +0.5 10,492 + +0.6 10,493 - +0.0 10,506 - +0.6 10,506 - -2.0 11,111 + Pf1N1B4_5589 0.46 -2.2 11,112 - Pf1N1B4_5589 0.46 +0.6 11,295 + Pf1N1B4_5589 0.60 -0.5 11,295 + Pf1N1B4_5589 0.60 -1.6 11,314 + Pf1N1B4_5589 0.62 -1.6 11,383 + Pf1N1B4_5589 0.67 -1.5 11,383 + Pf1N1B4_5589 0.67 -0.2
Or see this region's nucleotide sequence