Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5588

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5586 and Pf1N1B4_5587 overlap by 4 nucleotidesPf1N1B4_5587 and Pf1N1B4_5588 are separated by 10 nucleotidesPf1N1B4_5588 and Pf1N1B4_5589 are separated by 83 nucleotides Pf1N1B4_5586 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 7,744 to 8,790 _5586 Pf1N1B4_5587 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 8,787 to 9,737 _5587 Pf1N1B4_5588 - Transcriptional regulator for ferulate or vanillate catabolism, at 9,748 to 10,461 _5588 Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786 _5589 Position (kb) 9 10 11Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 9.265 kb on + strand, within Pf1N1B4_5587at 9.265 kb on + strand, within Pf1N1B4_5587at 9.265 kb on + strand, within Pf1N1B4_5587at 9.266 kb on - strandat 9.266 kb on - strandat 9.346 kb on + strand, within Pf1N1B4_5587at 9.346 kb on + strand, within Pf1N1B4_5587at 9.346 kb on + strand, within Pf1N1B4_5587at 9.346 kb on + strand, within Pf1N1B4_5587at 9.346 kb on + strand, within Pf1N1B4_5587at 9.347 kb on - strand, within Pf1N1B4_5587at 9.348 kb on + strand, within Pf1N1B4_5587at 9.348 kb on + strand, within Pf1N1B4_5587at 9.348 kb on + strand, within Pf1N1B4_5587at 9.348 kb on + strand, within Pf1N1B4_5587at 9.348 kb on + strand, within Pf1N1B4_5587at 9.349 kb on - strandat 9.349 kb on - strand, within Pf1N1B4_5587at 9.349 kb on - strand, within Pf1N1B4_5587at 9.349 kb on - strand, within Pf1N1B4_5587at 9.349 kb on - strand, within Pf1N1B4_5587at 9.547 kb on + strand, within Pf1N1B4_5587at 9.548 kb on - strand, within Pf1N1B4_5587at 9.739 kb on - strandat 9.750 kb on - strandat 9.914 kb on + strand, within Pf1N1B4_5588at 9.915 kb on - strand, within Pf1N1B4_5588at 10.093 kb on + strand, within Pf1N1B4_5588at 10.093 kb on + strand, within Pf1N1B4_5588at 10.094 kb on - strand, within Pf1N1B4_5588at 10.456 kb on - strandat 10.492 kb on + strandat 10.492 kb on + strandat 10.492 kb on + strandat 10.493 kb on - strandat 10.506 kb on - strandat 10.506 kb on - strandat 11.111 kb on + strand, within Pf1N1B4_5589at 11.112 kb on - strand, within Pf1N1B4_5589at 11.295 kb on + strand, within Pf1N1B4_5589at 11.295 kb on + strand, within Pf1N1B4_5589at 11.314 kb on + strand, within Pf1N1B4_5589at 11.383 kb on + strand, within Pf1N1B4_5589at 11.383 kb on + strand, within Pf1N1B4_5589

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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9,265 + Pf1N1B4_5587 0.50 -3.0
9,265 + Pf1N1B4_5587 0.50 -0.0
9,265 + Pf1N1B4_5587 0.50 -0.5
9,266 - +1.3
9,266 - -2.2
9,346 + Pf1N1B4_5587 0.59 -1.1
9,346 + Pf1N1B4_5587 0.59 -3.4
9,346 + Pf1N1B4_5587 0.59 -1.1
9,346 + Pf1N1B4_5587 0.59 -1.0
9,346 + Pf1N1B4_5587 0.59 -2.5
9,347 - Pf1N1B4_5587 0.59 +0.6
9,348 + Pf1N1B4_5587 0.59 -1.6
9,348 + Pf1N1B4_5587 0.59 +1.7
9,348 + Pf1N1B4_5587 0.59 -1.0
9,348 + Pf1N1B4_5587 0.59 +0.0
9,348 + Pf1N1B4_5587 0.59 -2.6
9,349 - +2.0
9,349 - Pf1N1B4_5587 0.59 +0.8
9,349 - Pf1N1B4_5587 0.59 -0.6
9,349 - Pf1N1B4_5587 0.59 -1.0
9,349 - Pf1N1B4_5587 0.59 -2.4
9,547 + Pf1N1B4_5587 0.80 -0.2
9,548 - Pf1N1B4_5587 0.80 -1.0
9,739 - -0.7
9,750 - -3.5
9,914 + Pf1N1B4_5588 0.23 -1.3
9,915 - Pf1N1B4_5588 0.23 -1.7
10,093 + Pf1N1B4_5588 0.48 -2.0
10,093 + Pf1N1B4_5588 0.48 -2.5
10,094 - Pf1N1B4_5588 0.48 -0.4
10,456 - -1.1
10,492 + -0.7
10,492 + +0.5
10,492 + +0.6
10,493 - +0.0
10,506 - +0.6
10,506 - -2.0
11,111 + Pf1N1B4_5589 0.46 -2.2
11,112 - Pf1N1B4_5589 0.46 +0.6
11,295 + Pf1N1B4_5589 0.60 -0.5
11,295 + Pf1N1B4_5589 0.60 -1.6
11,314 + Pf1N1B4_5589 0.62 -1.6
11,383 + Pf1N1B4_5589 0.67 -1.5
11,383 + Pf1N1B4_5589 0.67 -0.2

Or see this region's nucleotide sequence