Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5575 and Pf1N1B4_5576 are separated by 838 nucleotides Pf1N1B4_5576 and Pf1N1B4_5577 are separated by 217 nucleotides
Pf1N1B4_5575 - hypothetical protein, at 345,254 to 345,634
_5575
Pf1N1B4_5576 - hypothetical protein, at 346,473 to 346,823
_5576
Pf1N1B4_5577 - twitching motility protein PilG, at 347,041 to 347,319
_5577
Position (kb) 346
347 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 345.536 kb on + strand, within Pf1N1B4_5575 at 345.536 kb on + strand, within Pf1N1B4_5575 at 345.537 kb on - strand, within Pf1N1B4_5575 at 345.554 kb on - strand, within Pf1N1B4_5575 at 345.679 kb on + strand at 345.680 kb on - strand at 345.680 kb on - strand at 345.680 kb on - strand at 345.699 kb on - strand at 345.805 kb on - strand at 345.875 kb on + strand at 345.876 kb on - strand at 345.887 kb on + strand at 345.888 kb on - strand at 345.888 kb on - strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.957 kb on - strand at 345.957 kb on - strand at 345.963 kb on + strand at 345.963 kb on + strand at 345.963 kb on + strand at 345.964 kb on - strand at 345.964 kb on - strand at 346.013 kb on + strand at 346.013 kb on + strand at 346.013 kb on + strand at 346.019 kb on + strand at 346.019 kb on + strand at 346.019 kb on + strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.021 kb on + strand at 346.022 kb on - strand at 346.058 kb on + strand at 346.059 kb on - strand at 346.059 kb on - strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.162 kb on + strand at 346.162 kb on + strand at 346.163 kb on - strand at 346.171 kb on + strand at 346.171 kb on + strand at 346.171 kb on + strand at 346.172 kb on - strand at 346.202 kb on + strand at 346.231 kb on + strand at 346.257 kb on - strand at 346.351 kb on - strand at 346.351 kb on - strand at 346.664 kb on + strand, within Pf1N1B4_5576 at 346.670 kb on + strand, within Pf1N1B4_5576 at 346.670 kb on + strand, within Pf1N1B4_5576 at 346.671 kb on - strand at 346.767 kb on + strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.771 kb on - strand, within Pf1N1B4_5576 at 347.051 kb on + strand at 347.052 kb on - strand at 347.052 kb on - strand at 347.102 kb on + strand, within Pf1N1B4_5577 at 347.102 kb on + strand, within Pf1N1B4_5577 at 347.157 kb on + strand, within Pf1N1B4_5577 at 347.157 kb on + strand, within Pf1N1B4_5577 at 347.179 kb on - strand, within Pf1N1B4_5577 at 347.319 kb on + strand at 347.320 kb on - strand at 347.320 kb on - strand at 347.391 kb on + strand at 347.392 kb on - strand at 347.392 kb on - strand at 347.424 kb on + strand at 347.480 kb on + strand at 347.480 kb on + strand at 347.481 kb on - strand at 347.505 kb on + strand at 347.506 kb on - strand at 347.516 kb on - strand at 347.519 kb on + strand at 347.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 345,536 + Pf1N1B4_5575 0.74 +1.9 345,536 + Pf1N1B4_5575 0.74 +0.9 345,537 - Pf1N1B4_5575 0.74 +0.9 345,554 - Pf1N1B4_5575 0.79 -0.7 345,679 + -0.7 345,680 - +1.6 345,680 - -0.7 345,680 - +0.9 345,699 - -2.7 345,805 - -1.7 345,875 + -0.4 345,876 - -0.9 345,887 + -0.1 345,888 - -0.9 345,888 - +0.9 345,952 + +0.9 345,952 + +0.0 345,952 + +0.9 345,952 + -0.8 345,952 + -1.6 345,953 - +0.9 345,953 - +0.9 345,953 - -0.3 345,953 - -1.1 345,953 - -1.0 345,953 - +0.9 345,953 - -1.9 345,953 - -0.2 345,956 + -1.8 345,956 + -1.4 345,956 + -3.2 345,956 + -0.6 345,957 - -1.3 345,957 - -1.9 345,963 + -0.3 345,963 + -2.3 345,963 + -1.8 345,964 - -0.8 345,964 - -0.9 346,013 + -2.0 346,013 + -2.1 346,013 + -2.6 346,019 + -2.8 346,019 + -2.5 346,019 + -1.5 346,020 - -1.5 346,020 - -0.1 346,020 - -1.9 346,020 - -4.2 346,021 + +0.8 346,022 - +1.0 346,058 + +0.9 346,059 - +0.4 346,059 - +0.9 346,060 + -0.1 346,060 + -0.3 346,060 + -0.9 346,060 + -1.3 346,060 + -1.7 346,060 + +0.9 346,061 - -2.9 346,061 - +0.9 346,061 - +0.9 346,061 - +0.9 346,061 - +0.9 346,061 - -0.9 346,061 - -3.1 346,061 - -1.7 346,061 - -0.4 346,061 - -2.7 346,061 - -0.7 346,061 - +0.9 346,061 - -0.1 346,162 + +0.9 346,162 + -1.9 346,163 - -2.7 346,171 + +0.9 346,171 + +0.9 346,171 + -0.4 346,172 - -0.5 346,202 + +0.2 346,231 + -3.2 346,257 - -0.1 346,351 - +0.9 346,351 - -1.5 346,664 + Pf1N1B4_5576 0.54 -0.6 346,670 + Pf1N1B4_5576 0.56 -2.3 346,670 + Pf1N1B4_5576 0.56 -3.7 346,671 - -2.7 346,767 + Pf1N1B4_5576 0.84 -1.1 346,768 - Pf1N1B4_5576 0.84 +1.3 346,768 - Pf1N1B4_5576 0.84 -1.5 346,768 - Pf1N1B4_5576 0.84 -1.7 346,768 - Pf1N1B4_5576 0.84 +0.9 346,771 - Pf1N1B4_5576 0.85 -3.3 347,051 + -2.3 347,052 - +1.4 347,052 - +0.9 347,102 + Pf1N1B4_5577 0.22 -1.3 347,102 + Pf1N1B4_5577 0.22 +0.9 347,157 + Pf1N1B4_5577 0.42 -1.2 347,157 + Pf1N1B4_5577 0.42 -0.5 347,179 - Pf1N1B4_5577 0.49 +0.1 347,319 + -2.6 347,320 - +0.8 347,320 - +0.5 347,391 + -2.1 347,392 - +0.9 347,392 - -0.7 347,424 + +0.9 347,480 + +0.9 347,480 + +0.0 347,481 - -2.8 347,505 + -3.5 347,506 - -1.9 347,516 - -0.5 347,519 + +0.9 347,520 - +0.7
Or see this region's nucleotide sequence