Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5428 and Pf1N1B4_5429 are separated by 253 nucleotides Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides Pf1N1B4_5430 and Pf1N1B4_5431 are separated by 720 nucleotides
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Pf1N1B4_5431 - hypothetical protein, at 170,517 to 171,065
_5431
Position (kb) 168
169
170 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428 at 168.196 kb on + strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.197 kb on - strand, within Pf1N1B4_5428 at 168.201 kb on - strand, within Pf1N1B4_5428 at 168.381 kb on + strand, within Pf1N1B4_5428 at 168.391 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.415 kb on + strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.416 kb on - strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.419 kb on + strand, within Pf1N1B4_5428 at 168.420 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.524 kb on - strand, within Pf1N1B4_5428 at 168.580 kb on + strand at 168.581 kb on - strand at 168.660 kb on - strand at 168.728 kb on + strand at 168.729 kb on - strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.731 kb on + strand at 168.732 kb on - strand at 168.747 kb on + strand at 168.747 kb on + strand at 168.906 kb on - strand at 168.977 kb on + strand, within Pf1N1B4_5429 at 168.978 kb on - strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.103 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.175 kb on + strand, within Pf1N1B4_5429 at 169.176 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.304 kb on - strand, within Pf1N1B4_5429 at 169.329 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.337 kb on + strand, within Pf1N1B4_5429 at 169.338 kb on - strand, within Pf1N1B4_5429 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand at 169.681 kb on + strand, within Pf1N1B4_5430 at 169.682 kb on - strand, within Pf1N1B4_5430 at 169.747 kb on - strand at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.751 kb on - strand at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.809 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.899 kb on + strand at 169.899 kb on + strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.911 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.017 kb on - strand at 170.069 kb on + strand at 170.082 kb on + strand at 170.083 kb on - strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.190 kb on - strand at 170.190 kb on - strand at 170.244 kb on + strand at 170.244 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.282 kb on + strand at 170.368 kb on + strand at 170.368 kb on + strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 168,034 + Pf1N1B4_5428 0.38 -1.2 168,035 - Pf1N1B4_5428 0.38 -2.7 168,052 + Pf1N1B4_5428 0.40 -1.5 168,053 - Pf1N1B4_5428 0.40 +0.3 168,053 - +0.9 168,069 + Pf1N1B4_5428 0.42 -0.4 168,070 - Pf1N1B4_5428 0.42 +0.1 168,130 + Pf1N1B4_5428 0.48 -1.5 168,131 - Pf1N1B4_5428 0.48 -0.5 168,131 - Pf1N1B4_5428 0.48 +1.1 168,131 - Pf1N1B4_5428 0.48 +0.7 168,131 - Pf1N1B4_5428 0.48 -0.6 168,133 + Pf1N1B4_5428 0.48 +0.3 168,133 + Pf1N1B4_5428 0.48 -0.9 168,133 + Pf1N1B4_5428 0.48 -1.5 168,133 + Pf1N1B4_5428 0.48 +0.3 168,133 + Pf1N1B4_5428 0.48 +0.9 168,133 + Pf1N1B4_5428 0.48 -0.3 168,134 - Pf1N1B4_5428 0.48 +0.9 168,134 - Pf1N1B4_5428 0.48 -2.8 168,145 + Pf1N1B4_5428 0.49 -0.0 168,196 + Pf1N1B4_5428 0.54 -0.3 168,197 - Pf1N1B4_5428 0.55 -3.9 168,197 - Pf1N1B4_5428 0.55 -0.9 168,197 - Pf1N1B4_5428 0.55 +0.2 168,197 - Pf1N1B4_5428 0.55 -0.5 168,201 - Pf1N1B4_5428 0.55 +0.3 168,381 + Pf1N1B4_5428 0.73 +0.2 168,391 + Pf1N1B4_5428 0.74 -1.9 168,415 + Pf1N1B4_5428 0.77 -1.2 168,415 + Pf1N1B4_5428 0.77 +2.9 168,416 - Pf1N1B4_5428 0.77 -0.9 168,416 - Pf1N1B4_5428 0.77 +0.9 168,419 + Pf1N1B4_5428 0.77 -2.6 168,419 + Pf1N1B4_5428 0.77 -2.1 168,419 + Pf1N1B4_5428 0.77 -0.1 168,419 + Pf1N1B4_5428 0.77 -2.9 168,419 + Pf1N1B4_5428 0.77 -1.8 168,420 - Pf1N1B4_5428 0.77 -0.3 168,524 - Pf1N1B4_5428 0.88 -1.2 168,524 - Pf1N1B4_5428 0.88 +1.5 168,580 + -1.5 168,581 - +0.9 168,660 - -0.3 168,728 + +1.0 168,729 - +0.9 168,731 + -0.5 168,731 + -0.4 168,731 + -0.5 168,732 - -2.5 168,747 + -1.2 168,747 + -1.7 168,906 - -1.0 168,977 + Pf1N1B4_5429 0.12 -2.6 168,978 - Pf1N1B4_5429 0.12 -0.1 169,103 + Pf1N1B4_5429 0.32 -0.3 169,103 + Pf1N1B4_5429 0.32 +0.6 169,175 + Pf1N1B4_5429 0.43 -0.5 169,175 + Pf1N1B4_5429 0.43 -0.7 169,175 + Pf1N1B4_5429 0.43 -3.1 169,175 + Pf1N1B4_5429 0.43 +0.9 169,175 + Pf1N1B4_5429 0.43 +0.6 169,176 - Pf1N1B4_5429 0.43 +0.9 169,304 - Pf1N1B4_5429 0.63 +0.9 169,304 - Pf1N1B4_5429 0.63 -0.6 169,329 + Pf1N1B4_5429 0.67 -1.5 169,337 + Pf1N1B4_5429 0.68 +0.9 169,337 + Pf1N1B4_5429 0.68 -0.9 169,337 + Pf1N1B4_5429 0.68 +1.5 169,338 - Pf1N1B4_5429 0.68 -2.9 169,549 + -0.9 169,549 + -2.2 169,549 + -1.1 169,549 + +0.9 169,549 + +0.9 169,584 + +0.9 169,584 + -0.1 169,584 + -1.1 169,585 - -1.5 169,585 - -1.3 169,631 - -0.2 169,681 + Pf1N1B4_5430 0.31 -0.8 169,682 - Pf1N1B4_5430 0.32 -0.3 169,747 - +0.2 169,750 + Pf1N1B4_5430 0.72 +0.9 169,750 + Pf1N1B4_5430 0.72 -2.7 169,751 - +0.9 169,751 - Pf1N1B4_5430 0.73 -0.1 169,751 - Pf1N1B4_5430 0.73 +0.1 169,751 - Pf1N1B4_5430 0.73 -3.0 169,751 - Pf1N1B4_5430 0.73 -2.1 169,792 + -2.7 169,792 + +0.4 169,792 + -2.0 169,792 + -0.0 169,793 - -0.3 169,793 - +0.9 169,793 - -0.2 169,793 - -2.0 169,793 - -3.3 169,809 + -1.1 169,895 + -1.9 169,895 + -1.5 169,895 + +0.9 169,895 + -1.7 169,895 + -3.4 169,896 - -0.1 169,896 - -0.5 169,896 - -1.8 169,896 - +0.9 169,896 - +0.6 169,896 - -2.6 169,896 - -2.6 169,896 - -2.3 169,899 + -0.1 169,899 + +0.9 169,900 - +0.9 169,900 - +2.7 169,900 - +0.2 169,911 + -1.7 170,016 + +0.9 170,016 + +0.5 170,016 + +0.1 170,017 - +0.9 170,069 + +0.9 170,082 + +0.9 170,083 - +0.9 170,156 + -1.9 170,156 + -3.2 170,156 + -1.3 170,157 - -1.5 170,157 - -0.7 170,157 - -0.7 170,157 - -0.6 170,190 - +0.9 170,190 - -0.8 170,244 + +0.5 170,244 + -1.1 170,256 + -1.7 170,256 + -0.6 170,256 + -1.0 170,282 + +0.9 170,368 + -0.7 170,368 + -0.5 170,369 - +0.1 170,369 - +0.9 170,369 - -0.7 170,369 - +0.5 170,369 - -2.5
Or see this region's nucleotide sequence