Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5361

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5360 and Pf1N1B4_5361 are separated by 390 nucleotidesPf1N1B4_5361 and Pf1N1B4_5362 are separated by 110 nucleotidesPf1N1B4_5362 and Pf1N1B4_5363 are separated by 56 nucleotides Pf1N1B4_5360 - hypothetical protein, at 95,711 to 96,091 _5360 Pf1N1B4_5361 - hypothetical protein, at 96,482 to 96,676 _5361 Pf1N1B4_5362 - hypothetical protein, at 96,787 to 97,581 _5362 Pf1N1B4_5363 - hypothetical protein, at 97,638 to 98,639 _5363 Position (kb) 96 97Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 95.620 kb on + strandat 95.620 kb on + strandat 95.734 kb on - strandat 95.807 kb on + strand, within Pf1N1B4_5360at 95.807 kb on + strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strandat 95.808 kb on - strandat 95.808 kb on - strandat 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.808 kb on - strand, within Pf1N1B4_5360at 95.834 kb on + strand, within Pf1N1B4_5360at 95.835 kb on - strand, within Pf1N1B4_5360at 95.835 kb on - strand, within Pf1N1B4_5360at 95.912 kb on + strand, within Pf1N1B4_5360at 95.912 kb on + strand, within Pf1N1B4_5360at 95.912 kb on + strand, within Pf1N1B4_5360at 95.913 kb on - strand, within Pf1N1B4_5360at 95.913 kb on - strand, within Pf1N1B4_5360at 95.913 kb on - strand, within Pf1N1B4_5360at 96.042 kb on - strand, within Pf1N1B4_5360at 96.042 kb on - strand, within Pf1N1B4_5360at 96.042 kb on - strand, within Pf1N1B4_5360at 96.042 kb on - strand, within Pf1N1B4_5360at 96.042 kb on - strand, within Pf1N1B4_5360at 96.531 kb on + strand, within Pf1N1B4_5361at 96.531 kb on + strand, within Pf1N1B4_5361at 96.531 kb on + strand, within Pf1N1B4_5361at 96.532 kb on - strand, within Pf1N1B4_5361at 96.532 kb on - strandat 96.625 kb on - strand, within Pf1N1B4_5361at 96.669 kb on + strandat 96.669 kb on + strandat 96.669 kb on + strandat 96.670 kb on - strandat 96.670 kb on - strandat 96.843 kb on + strandat 96.882 kb on + strand, within Pf1N1B4_5362at 96.883 kb on - strand, within Pf1N1B4_5362at 96.883 kb on - strand, within Pf1N1B4_5362at 96.974 kb on - strand, within Pf1N1B4_5362at 96.974 kb on - strand, within Pf1N1B4_5362at 97.075 kb on + strand, within Pf1N1B4_5362at 97.076 kb on - strand, within Pf1N1B4_5362at 97.076 kb on - strand, within Pf1N1B4_5362at 97.080 kb on + strand, within Pf1N1B4_5362at 97.080 kb on + strand, within Pf1N1B4_5362at 97.081 kb on - strand, within Pf1N1B4_5362at 97.117 kb on + strand, within Pf1N1B4_5362at 97.117 kb on + strandat 97.118 kb on - strand, within Pf1N1B4_5362at 97.154 kb on - strand, within Pf1N1B4_5362at 97.154 kb on - strand, within Pf1N1B4_5362at 97.191 kb on + strandat 97.191 kb on + strandat 97.192 kb on - strandat 97.204 kb on - strandat 97.223 kb on + strand, within Pf1N1B4_5362at 97.224 kb on - strand, within Pf1N1B4_5362at 97.241 kb on + strand, within Pf1N1B4_5362at 97.241 kb on + strand, within Pf1N1B4_5362at 97.251 kb on - strand, within Pf1N1B4_5362at 97.251 kb on - strand, within Pf1N1B4_5362at 97.272 kb on + strand, within Pf1N1B4_5362at 97.272 kb on + strand, within Pf1N1B4_5362at 97.288 kb on + strand, within Pf1N1B4_5362at 97.288 kb on + strand, within Pf1N1B4_5362at 97.288 kb on + strand, within Pf1N1B4_5362at 97.289 kb on - strand, within Pf1N1B4_5362at 97.289 kb on - strand, within Pf1N1B4_5362at 97.289 kb on - strandat 97.289 kb on - strand, within Pf1N1B4_5362at 97.289 kb on - strand, within Pf1N1B4_5362at 97.402 kb on + strand, within Pf1N1B4_5362at 97.402 kb on + strand, within Pf1N1B4_5362at 97.464 kb on + strand, within Pf1N1B4_5362at 97.464 kb on + strand, within Pf1N1B4_5362at 97.464 kb on + strandat 97.464 kb on + strandat 97.464 kb on + strand, within Pf1N1B4_5362at 97.465 kb on - strand, within Pf1N1B4_5362at 97.465 kb on - strand, within Pf1N1B4_5362at 97.465 kb on - strand, within Pf1N1B4_5362at 97.581 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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95,620 + -0.8
95,620 + +0.5
95,734 - -2.1
95,807 + Pf1N1B4_5360 0.25 +0.9
95,807 + Pf1N1B4_5360 0.25 -0.1
95,808 - Pf1N1B4_5360 0.25 -1.4
95,808 - Pf1N1B4_5360 0.25 -1.3
95,808 - +0.7
95,808 - -1.1
95,808 - +0.9
95,808 - Pf1N1B4_5360 0.25 -3.4
95,808 - Pf1N1B4_5360 0.25 -3.3
95,808 - Pf1N1B4_5360 0.25 +0.9
95,808 - Pf1N1B4_5360 0.25 -2.1
95,808 - Pf1N1B4_5360 0.25 -0.6
95,808 - Pf1N1B4_5360 0.25 +0.9
95,834 + Pf1N1B4_5360 0.32 -1.4
95,835 - Pf1N1B4_5360 0.33 -2.4
95,835 - Pf1N1B4_5360 0.33 -0.8
95,912 + Pf1N1B4_5360 0.53 +0.9
95,912 + Pf1N1B4_5360 0.53 -1.4
95,912 + Pf1N1B4_5360 0.53 -0.9
95,913 - Pf1N1B4_5360 0.53 -0.1
95,913 - Pf1N1B4_5360 0.53 +0.3
95,913 - Pf1N1B4_5360 0.53 +0.5
96,042 - Pf1N1B4_5360 0.87 +0.9
96,042 - Pf1N1B4_5360 0.87 -2.1
96,042 - Pf1N1B4_5360 0.87 +0.4
96,042 - Pf1N1B4_5360 0.87 -0.1
96,042 - Pf1N1B4_5360 0.87 -1.0
96,531 + Pf1N1B4_5361 0.25 +0.9
96,531 + Pf1N1B4_5361 0.25 -2.1
96,531 + Pf1N1B4_5361 0.25 +0.9
96,532 - Pf1N1B4_5361 0.26 +0.9
96,532 - -2.3
96,625 - Pf1N1B4_5361 0.73 -3.4
96,669 + +0.2
96,669 + +0.9
96,669 + +0.2
96,670 - -0.0
96,670 - +0.1
96,843 + -1.1
96,882 + Pf1N1B4_5362 0.12 -1.0
96,883 - Pf1N1B4_5362 0.12 -0.7
96,883 - Pf1N1B4_5362 0.12 +0.1
96,974 - Pf1N1B4_5362 0.24 -2.1
96,974 - Pf1N1B4_5362 0.24 +0.9
97,075 + Pf1N1B4_5362 0.36 +0.9
97,076 - Pf1N1B4_5362 0.36 -1.7
97,076 - Pf1N1B4_5362 0.36 -1.0
97,080 + Pf1N1B4_5362 0.37 +0.9
97,080 + Pf1N1B4_5362 0.37 +0.9
97,081 - Pf1N1B4_5362 0.37 +0.9
97,117 + Pf1N1B4_5362 0.42 +0.2
97,117 + +0.1
97,118 - Pf1N1B4_5362 0.42 +0.9
97,154 - Pf1N1B4_5362 0.46 -4.0
97,154 - Pf1N1B4_5362 0.46 -1.8
97,191 + +0.9
97,191 + +0.9
97,192 - -0.1
97,204 - -2.7
97,223 + Pf1N1B4_5362 0.55 -0.6
97,224 - Pf1N1B4_5362 0.55 -0.7
97,241 + Pf1N1B4_5362 0.57 -0.7
97,241 + Pf1N1B4_5362 0.57 +1.1
97,251 - Pf1N1B4_5362 0.58 -1.9
97,251 - Pf1N1B4_5362 0.58 -0.3
97,272 + Pf1N1B4_5362 0.61 -1.7
97,272 + Pf1N1B4_5362 0.61 -0.3
97,288 + Pf1N1B4_5362 0.63 -0.5
97,288 + Pf1N1B4_5362 0.63 -0.5
97,288 + Pf1N1B4_5362 0.63 -2.3
97,289 - Pf1N1B4_5362 0.63 -0.7
97,289 - Pf1N1B4_5362 0.63 +2.0
97,289 - -3.0
97,289 - Pf1N1B4_5362 0.63 -2.4
97,289 - Pf1N1B4_5362 0.63 +0.9
97,402 + Pf1N1B4_5362 0.77 -0.4
97,402 + Pf1N1B4_5362 0.77 -2.1
97,464 + Pf1N1B4_5362 0.85 +0.9
97,464 + Pf1N1B4_5362 0.85 -0.9
97,464 + -1.9
97,464 + +0.9
97,464 + Pf1N1B4_5362 0.85 +0.9
97,465 - Pf1N1B4_5362 0.85 -0.1
97,465 - Pf1N1B4_5362 0.85 +0.9
97,465 - Pf1N1B4_5362 0.85 -0.3
97,581 + +0.9

Or see this region's nucleotide sequence