Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5305 and Pf1N1B4_5306 are separated by 93 nucleotides Pf1N1B4_5306 and Pf1N1B4_5307 are separated by 658 nucleotides
Pf1N1B4_5305 - hypothetical protein, at 56,862 to 57,851
_5305
Pf1N1B4_5306 - hypothetical protein, at 57,945 to 58,142
_5306
Pf1N1B4_5307 - hypothetical protein, at 58,801 to 59,055
_5307
Position (kb) 57
58
59 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 56.987 kb on + strand at 56.988 kb on - strand, within Pf1N1B4_5305 at 56.988 kb on - strand, within Pf1N1B4_5305 at 56.988 kb on - strand, within Pf1N1B4_5305 at 57.090 kb on - strand, within Pf1N1B4_5305 at 57.090 kb on - strand, within Pf1N1B4_5305 at 57.156 kb on - strand, within Pf1N1B4_5305 at 57.234 kb on - strand, within Pf1N1B4_5305 at 57.285 kb on + strand at 57.285 kb on + strand, within Pf1N1B4_5305 at 57.286 kb on - strand, within Pf1N1B4_5305 at 57.372 kb on + strand, within Pf1N1B4_5305 at 57.373 kb on - strand, within Pf1N1B4_5305 at 57.558 kb on + strand, within Pf1N1B4_5305 at 57.558 kb on + strand, within Pf1N1B4_5305 at 57.558 kb on + strand, within Pf1N1B4_5305 at 57.558 kb on + strand, within Pf1N1B4_5305 at 57.559 kb on - strand, within Pf1N1B4_5305 at 57.598 kb on + strand, within Pf1N1B4_5305 at 57.598 kb on + strand, within Pf1N1B4_5305 at 57.599 kb on - strand, within Pf1N1B4_5305 at 57.643 kb on - strand, within Pf1N1B4_5305 at 57.673 kb on + strand, within Pf1N1B4_5305 at 57.673 kb on + strand, within Pf1N1B4_5305 at 57.673 kb on + strand, within Pf1N1B4_5305 at 57.673 kb on + strand, within Pf1N1B4_5305 at 57.694 kb on + strand, within Pf1N1B4_5305 at 57.694 kb on + strand, within Pf1N1B4_5305 at 57.720 kb on + strand, within Pf1N1B4_5305 at 57.721 kb on - strand, within Pf1N1B4_5305 at 57.777 kb on + strand at 57.777 kb on + strand at 57.778 kb on - strand at 57.778 kb on - strand at 57.778 kb on - strand at 57.932 kb on - strand at 57.981 kb on + strand, within Pf1N1B4_5306 at 57.982 kb on - strand, within Pf1N1B4_5306 at 58.037 kb on + strand, within Pf1N1B4_5306 at 58.042 kb on - strand, within Pf1N1B4_5306 at 58.042 kb on - strand, within Pf1N1B4_5306 at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.140 kb on + strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.141 kb on - strand at 58.194 kb on + strand at 58.194 kb on + strand at 58.195 kb on - strand at 58.262 kb on + strand at 58.262 kb on + strand at 58.263 kb on - strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.271 kb on + strand at 58.272 kb on - strand at 58.272 kb on - strand at 58.272 kb on - strand at 58.275 kb on + strand at 58.275 kb on + strand at 58.323 kb on + strand at 58.323 kb on + strand at 58.323 kb on + strand at 58.323 kb on + strand at 58.324 kb on - strand at 58.324 kb on - strand at 58.534 kb on + strand at 58.535 kb on - strand at 58.535 kb on - strand at 58.595 kb on - strand at 58.595 kb on - strand at 58.744 kb on + strand at 58.744 kb on + strand at 58.744 kb on + strand at 58.744 kb on + strand at 58.745 kb on - strand at 58.745 kb on - strand at 58.770 kb on + strand at 58.770 kb on + strand at 58.786 kb on + strand at 58.966 kb on + strand, within Pf1N1B4_5307 at 58.966 kb on + strand, within Pf1N1B4_5307 at 58.967 kb on - strand at 58.967 kb on - strand, within Pf1N1B4_5307 at 59.002 kb on + strand, within Pf1N1B4_5307 at 59.002 kb on + strand, within Pf1N1B4_5307 at 59.003 kb on - strand, within Pf1N1B4_5307 at 59.063 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 56,987 + -2.4 56,988 - Pf1N1B4_5305 0.13 -2.1 56,988 - Pf1N1B4_5305 0.13 -1.7 56,988 - Pf1N1B4_5305 0.13 +0.0 57,090 - Pf1N1B4_5305 0.23 -1.1 57,090 - Pf1N1B4_5305 0.23 +0.9 57,156 - Pf1N1B4_5305 0.30 +0.9 57,234 - Pf1N1B4_5305 0.38 +0.9 57,285 + -2.1 57,285 + Pf1N1B4_5305 0.43 -0.6 57,286 - Pf1N1B4_5305 0.43 -2.3 57,372 + Pf1N1B4_5305 0.52 +0.2 57,373 - Pf1N1B4_5305 0.52 +0.9 57,558 + Pf1N1B4_5305 0.70 -0.4 57,558 + Pf1N1B4_5305 0.70 -3.1 57,558 + Pf1N1B4_5305 0.70 +0.9 57,558 + Pf1N1B4_5305 0.70 -0.6 57,559 - Pf1N1B4_5305 0.70 -0.2 57,598 + Pf1N1B4_5305 0.74 +0.3 57,598 + Pf1N1B4_5305 0.74 -0.3 57,599 - Pf1N1B4_5305 0.74 +0.5 57,643 - Pf1N1B4_5305 0.79 +0.9 57,673 + Pf1N1B4_5305 0.82 +0.9 57,673 + Pf1N1B4_5305 0.82 -1.6 57,673 + Pf1N1B4_5305 0.82 -1.3 57,673 + Pf1N1B4_5305 0.82 +0.9 57,694 + Pf1N1B4_5305 0.84 -0.8 57,694 + Pf1N1B4_5305 0.84 -0.3 57,720 + Pf1N1B4_5305 0.87 -0.0 57,721 - Pf1N1B4_5305 0.87 +0.1 57,777 + -0.5 57,777 + -0.1 57,778 - -0.8 57,778 - -0.1 57,778 - -1.0 57,932 - -1.9 57,981 + Pf1N1B4_5306 0.18 -0.7 57,982 - Pf1N1B4_5306 0.19 -1.6 58,037 + Pf1N1B4_5306 0.46 -2.1 58,042 - Pf1N1B4_5306 0.49 -2.1 58,042 - Pf1N1B4_5306 0.49 +0.9 58,140 + +0.9 58,140 + -2.4 58,140 + +0.9 58,140 + -1.4 58,140 + -0.5 58,140 + +0.9 58,140 + +0.9 58,140 + -3.0 58,140 + -0.8 58,140 + +0.3 58,140 + +2.3 58,140 + -1.8 58,140 + -1.2 58,141 - -0.4 58,141 - -1.9 58,141 - -0.0 58,141 - +0.9 58,141 - -2.8 58,141 - -0.1 58,141 - -0.3 58,141 - -1.3 58,194 + -0.6 58,194 + -0.7 58,195 - +0.9 58,262 + +0.9 58,262 + -0.6 58,263 - +0.3 58,271 + +0.3 58,271 + -1.1 58,271 + +0.9 58,271 + -1.4 58,271 + +1.5 58,271 + -2.6 58,271 + +0.9 58,272 - -3.8 58,272 - -0.3 58,272 - +0.9 58,275 + -1.9 58,275 + +0.9 58,323 + -0.2 58,323 + -0.6 58,323 + +0.9 58,323 + -1.3 58,324 - -0.9 58,324 - -0.7 58,534 + +0.9 58,535 - +0.9 58,535 - +0.8 58,595 - +0.9 58,595 - -0.5 58,744 + -0.1 58,744 + -1.9 58,744 + -0.2 58,744 + +0.6 58,745 - +0.9 58,745 - -1.0 58,770 + -0.3 58,770 + -3.9 58,786 + -0.1 58,966 + Pf1N1B4_5307 0.65 -1.2 58,966 + Pf1N1B4_5307 0.65 -0.4 58,967 - +0.9 58,967 - Pf1N1B4_5307 0.65 +0.9 59,002 + Pf1N1B4_5307 0.79 +0.9 59,002 + Pf1N1B4_5307 0.79 -0.5 59,003 - Pf1N1B4_5307 0.79 -0.4 59,063 + +0.5
Or see this region's nucleotide sequence