Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5252

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotidesPf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368 _5251 Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272 _5252 Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045 _5253 Position (kb) 10 11 12Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 9.558 kb on + strandat 9.558 kb on + strandat 9.596 kb on + strandat 9.597 kb on - strandat 9.640 kb on - strand, within Pf1N1B4_5251at 9.640 kb on - strand, within Pf1N1B4_5251at 9.640 kb on - strand, within Pf1N1B4_5251at 9.640 kb on - strand, within Pf1N1B4_5251at 9.640 kb on - strand, within Pf1N1B4_5251at 9.640 kb on - strand, within Pf1N1B4_5251at 9.712 kb on + strand, within Pf1N1B4_5251at 9.920 kb on + strandat 9.920 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.136 kb on + strand, within Pf1N1B4_5251at 10.137 kb on - strand, within Pf1N1B4_5251at 10.137 kb on - strand, within Pf1N1B4_5251at 10.137 kb on - strandat 10.137 kb on - strandat 10.357 kb on - strandat 10.384 kb on + strandat 10.401 kb on - strandat 10.401 kb on - strandat 10.453 kb on - strandat 10.453 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.552 kb on - strandat 10.733 kb on + strand, within Pf1N1B4_5252at 10.780 kb on - strand, within Pf1N1B4_5252at 10.803 kb on + strand, within Pf1N1B4_5252at 10.803 kb on + strand, within Pf1N1B4_5252at 10.819 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.841 kb on - strand, within Pf1N1B4_5252at 10.929 kb on + strand, within Pf1N1B4_5252at 11.130 kb on + strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.134 kb on + strand, within Pf1N1B4_5252at 11.134 kb on + strand, within Pf1N1B4_5252at 11.159 kb on + strand, within Pf1N1B4_5252at 11.205 kb on + strandat 11.206 kb on - strandat 11.206 kb on - strandat 11.206 kb on - strandat 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.409 kb on - strand, within Pf1N1B4_5253at 11.423 kb on + strand, within Pf1N1B4_5253at 11.423 kb on + strand, within Pf1N1B4_5253at 11.424 kb on - strand, within Pf1N1B4_5253at 11.432 kb on + strand, within Pf1N1B4_5253at 11.432 kb on + strandat 11.433 kb on - strand, within Pf1N1B4_5253at 11.790 kb on - strandat 11.790 kb on - strand, within Pf1N1B4_5253at 11.864 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.921 kb on - strand, within Pf1N1B4_5253at 11.921 kb on - strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.925 kb on - strand, within Pf1N1B4_5253at 11.925 kb on - strand, within Pf1N1B4_5253at 11.969 kb on + strandat 11.969 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.034 kb on - strandat 12.034 kb on - strandat 12.034 kb on - strandat 12.068 kb on - strandat 12.068 kb on - strandat 12.153 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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9,558 + -1.4
9,558 + -2.3
9,596 + -1.7
9,597 - +0.9
9,640 - Pf1N1B4_5251 0.14 -0.7
9,640 - Pf1N1B4_5251 0.14 -2.1
9,640 - Pf1N1B4_5251 0.14 +0.5
9,640 - Pf1N1B4_5251 0.14 +0.9
9,640 - Pf1N1B4_5251 0.14 -2.7
9,640 - Pf1N1B4_5251 0.14 +0.9
9,712 + Pf1N1B4_5251 0.23 -2.1
9,920 + +0.9
9,920 + Pf1N1B4_5251 0.47 -2.9
10,136 + Pf1N1B4_5251 0.73 +0.9
10,136 + Pf1N1B4_5251 0.73 -2.0
10,136 + Pf1N1B4_5251 0.73 +0.9
10,136 + Pf1N1B4_5251 0.73 -0.3
10,136 + Pf1N1B4_5251 0.73 -2.6
10,136 + Pf1N1B4_5251 0.73 -1.0
10,136 + Pf1N1B4_5251 0.73 +0.9
10,136 + Pf1N1B4_5251 0.73 -1.3
10,137 - Pf1N1B4_5251 0.73 -1.9
10,137 - Pf1N1B4_5251 0.73 -1.1
10,137 - -1.3
10,137 - +0.3
10,357 - -0.2
10,384 + -2.0
10,401 - +0.9
10,401 - +0.9
10,453 - -1.7
10,453 - -0.8
10,496 - -1.7
10,496 - +0.9
10,496 - -2.6
10,496 - +0.9
10,500 - +0.5
10,500 - +0.9
10,500 - -1.9
10,500 - +0.4
10,552 - -0.3
10,733 + Pf1N1B4_5252 0.27 +0.0
10,780 - Pf1N1B4_5252 0.33 -0.3
10,803 + Pf1N1B4_5252 0.36 -1.2
10,803 + Pf1N1B4_5252 0.36 +0.9
10,819 + Pf1N1B4_5252 0.38 -1.4
10,821 + Pf1N1B4_5252 0.39 -2.7
10,821 + Pf1N1B4_5252 0.39 -0.2
10,821 + Pf1N1B4_5252 0.39 +0.1
10,822 - Pf1N1B4_5252 0.39 +0.9
10,822 - Pf1N1B4_5252 0.39 -0.9
10,822 - Pf1N1B4_5252 0.39 +0.9
10,841 - Pf1N1B4_5252 0.41 +0.9
10,929 + Pf1N1B4_5252 0.53 -1.1
11,130 + Pf1N1B4_5252 0.81 -0.9
11,131 - Pf1N1B4_5252 0.81 +0.1
11,131 - Pf1N1B4_5252 0.81 +1.0
11,131 - Pf1N1B4_5252 0.81 +1.9
11,134 + Pf1N1B4_5252 0.81 +0.9
11,134 + Pf1N1B4_5252 0.81 -3.0
11,159 + Pf1N1B4_5252 0.84 -0.2
11,205 + +0.9
11,206 - -3.6
11,206 - -1.0
11,206 - -1.5
11,408 + Pf1N1B4_5253 0.16 +0.9
11,408 + Pf1N1B4_5253 0.16 -1.8
11,408 + Pf1N1B4_5253 0.16 -1.1
11,408 + Pf1N1B4_5253 0.16 -1.1
11,409 - Pf1N1B4_5253 0.16 -1.5
11,423 + Pf1N1B4_5253 0.18 -3.0
11,423 + Pf1N1B4_5253 0.18 -0.1
11,424 - Pf1N1B4_5253 0.18 +0.9
11,432 + Pf1N1B4_5253 0.19 -0.1
11,432 + -0.5
11,433 - Pf1N1B4_5253 0.20 +0.9
11,790 - +0.4
11,790 - Pf1N1B4_5253 0.66 -2.4
11,864 + Pf1N1B4_5253 0.76 +0.4
11,920 + Pf1N1B4_5253 0.83 -0.2
11,920 + Pf1N1B4_5253 0.83 -2.4
11,920 + Pf1N1B4_5253 0.83 -1.5
11,920 + Pf1N1B4_5253 0.83 -1.1
11,921 - Pf1N1B4_5253 0.84 +0.9
11,921 - Pf1N1B4_5253 0.84 -3.1
11,924 + Pf1N1B4_5253 0.84 -1.9
11,924 + Pf1N1B4_5253 0.84 -1.4
11,924 + Pf1N1B4_5253 0.84 +0.1
11,925 - Pf1N1B4_5253 0.84 -0.1
11,925 - Pf1N1B4_5253 0.84 -0.4
11,969 + -2.1
11,969 + -2.4
12,033 + -0.1
12,033 + +0.9
12,033 + +1.3
12,033 + -1.9
12,034 - +1.2
12,034 - +0.2
12,034 - -1.4
12,068 - -0.5
12,068 - -2.4
12,153 + +0.9

Or see this region's nucleotide sequence