Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3004 and Pf1N1B4_5216 are separated by 72 nucleotides Pf1N1B4_5216 and Pf1N1B4_3005 are separated by 107 nucleotides
Pf1N1B4_3004 - Integrase, at 3,377,587 to 3,378,840
_3004
Pf1N1B4_5216 - tRNA-Leu-CAA, at 3,378,913 to 3,378,994
_5216
Pf1N1B4_3005 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 3,379,102 to 3,380,166
_3005
Position (kb)
3378
3379 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3378.005 kb on - strand, within Pf1N1B4_3004 at 3378.005 kb on - strand, within Pf1N1B4_3004 at 3378.115 kb on - strand, within Pf1N1B4_3004 at 3378.166 kb on + strand, within Pf1N1B4_3004 at 3378.173 kb on - strand, within Pf1N1B4_3004 at 3378.173 kb on - strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.322 kb on + strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.323 kb on - strand, within Pf1N1B4_3004 at 3378.338 kb on + strand, within Pf1N1B4_3004 at 3378.339 kb on - strand, within Pf1N1B4_3004 at 3378.339 kb on - strand, within Pf1N1B4_3004 at 3378.339 kb on - strand, within Pf1N1B4_3004 at 3378.339 kb on - strand, within Pf1N1B4_3004 at 3378.496 kb on + strand, within Pf1N1B4_3004 at 3378.496 kb on + strand, within Pf1N1B4_3004 at 3378.496 kb on + strand, within Pf1N1B4_3004 at 3378.496 kb on + strand at 3378.497 kb on - strand, within Pf1N1B4_3004 at 3378.497 kb on - strand, within Pf1N1B4_3004 at 3378.541 kb on + strand, within Pf1N1B4_3004 at 3378.541 kb on + strand, within Pf1N1B4_3004 at 3378.541 kb on + strand, within Pf1N1B4_3004 at 3378.541 kb on + strand, within Pf1N1B4_3004 at 3378.541 kb on + strand, within Pf1N1B4_3004 at 3378.542 kb on - strand, within Pf1N1B4_3004 at 3378.542 kb on - strand at 3378.542 kb on - strand, within Pf1N1B4_3004 at 3378.542 kb on - strand, within Pf1N1B4_3004 at 3378.596 kb on + strand, within Pf1N1B4_3004 at 3378.616 kb on - strand, within Pf1N1B4_3004 at 3378.616 kb on - strand, within Pf1N1B4_3004 at 3378.641 kb on + strand, within Pf1N1B4_3004 at 3378.641 kb on + strand, within Pf1N1B4_3004 at 3378.641 kb on + strand, within Pf1N1B4_3004 at 3378.779 kb on + strand at 3378.779 kb on + strand at 3378.780 kb on - strand at 3378.800 kb on - strand at 3378.824 kb on + strand at 3378.824 kb on + strand at 3378.824 kb on + strand at 3378.825 kb on - strand at 3378.825 kb on - strand at 3378.828 kb on + strand at 3378.828 kb on + strand at 3378.887 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.907 kb on - strand at 3378.909 kb on - strand at 3378.909 kb on - strand at 3378.909 kb on - strand at 3378.909 kb on - strand at 3378.909 kb on - strand at 3378.909 kb on - strand at 3379.042 kb on + strand at 3379.043 kb on - strand at 3379.043 kb on - strand at 3379.043 kb on - strand at 3379.043 kb on - strand at 3379.087 kb on + strand at 3379.088 kb on - strand at 3379.119 kb on + strand at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.543 kb on + strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.544 kb on - strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.545 kb on + strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.546 kb on - strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.547 kb on + strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.548 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.595 kb on - strand, within Pf1N1B4_3005 at 3379.858 kb on - strand, within Pf1N1B4_3005 at 3379.924 kb on - strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.929 kb on + strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005 at 3379.930 kb on - strand, within Pf1N1B4_3005
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 3,378,005 - Pf1N1B4_3004 0.33 +0.1 3,378,005 - Pf1N1B4_3004 0.33 +0.7 3,378,115 - Pf1N1B4_3004 0.42 -0.8 3,378,166 + Pf1N1B4_3004 0.46 -3.1 3,378,173 - Pf1N1B4_3004 0.47 -1.4 3,378,173 - Pf1N1B4_3004 0.47 -4.0 3,378,322 + Pf1N1B4_3004 0.59 -2.5 3,378,322 + Pf1N1B4_3004 0.59 -2.1 3,378,322 + Pf1N1B4_3004 0.59 -2.9 3,378,322 + Pf1N1B4_3004 0.59 -0.7 3,378,322 + Pf1N1B4_3004 0.59 -0.8 3,378,322 + Pf1N1B4_3004 0.59 +0.7 3,378,322 + Pf1N1B4_3004 0.59 -0.6 3,378,322 + Pf1N1B4_3004 0.59 +1.3 3,378,323 - Pf1N1B4_3004 0.59 +0.7 3,378,323 - Pf1N1B4_3004 0.59 +0.7 3,378,323 - Pf1N1B4_3004 0.59 -1.8 3,378,323 - Pf1N1B4_3004 0.59 +0.7 3,378,323 - Pf1N1B4_3004 0.59 +0.3 3,378,323 - Pf1N1B4_3004 0.59 +0.3 3,378,338 + Pf1N1B4_3004 0.60 -0.9 3,378,339 - Pf1N1B4_3004 0.60 -1.3 3,378,339 - Pf1N1B4_3004 0.60 -0.9 3,378,339 - Pf1N1B4_3004 0.60 +0.7 3,378,339 - Pf1N1B4_3004 0.60 -0.7 3,378,496 + Pf1N1B4_3004 0.72 -0.2 3,378,496 + Pf1N1B4_3004 0.72 -0.3 3,378,496 + Pf1N1B4_3004 0.72 +0.7 3,378,496 + +1.7 3,378,497 - Pf1N1B4_3004 0.73 -0.6 3,378,497 - Pf1N1B4_3004 0.73 -1.0 3,378,541 + Pf1N1B4_3004 0.76 -0.4 3,378,541 + Pf1N1B4_3004 0.76 -1.6 3,378,541 + Pf1N1B4_3004 0.76 +0.9 3,378,541 + Pf1N1B4_3004 0.76 -2.3 3,378,541 + Pf1N1B4_3004 0.76 -0.7 3,378,542 - Pf1N1B4_3004 0.76 -2.1 3,378,542 - +0.7 3,378,542 - Pf1N1B4_3004 0.76 -3.0 3,378,542 - Pf1N1B4_3004 0.76 -0.3 3,378,596 + Pf1N1B4_3004 0.80 +0.7 3,378,616 - Pf1N1B4_3004 0.82 -0.3 3,378,616 - Pf1N1B4_3004 0.82 -0.3 3,378,641 + Pf1N1B4_3004 0.84 +0.7 3,378,641 + Pf1N1B4_3004 0.84 +0.7 3,378,641 + Pf1N1B4_3004 0.84 -0.3 3,378,779 + -0.3 3,378,779 + +0.7 3,378,780 - -3.5 3,378,800 - -1.8 3,378,824 + -1.4 3,378,824 + -3.0 3,378,824 + +1.8 3,378,825 - -1.9 3,378,825 - -0.1 3,378,828 + +1.0 3,378,828 + -0.0 3,378,887 - -1.5 3,378,907 - -0.9 3,378,907 - -1.3 3,378,907 - -1.0 3,378,907 - -0.3 3,378,907 - +0.1 3,378,907 - -0.0 3,378,907 - -0.3 3,378,907 - -0.9 3,378,907 - -0.2 3,378,907 - -3.5 3,378,907 - -3.4 3,378,907 - -1.9 3,378,907 - -1.6 3,378,907 - -0.9 3,378,907 - +0.7 3,378,907 - +0.7 3,378,907 - +0.7 3,378,907 - +0.1 3,378,907 - +0.3 3,378,909 - -3.0 3,378,909 - -3.7 3,378,909 - +0.7 3,378,909 - -1.5 3,378,909 - -1.9 3,378,909 - -1.1 3,379,042 + -1.6 3,379,043 - -0.3 3,379,043 - -4.2 3,379,043 - +0.7 3,379,043 - -0.3 3,379,087 + -0.3 3,379,088 - -1.9 3,379,119 + -0.1 3,379,543 + Pf1N1B4_3005 0.41 +0.7 3,379,543 + Pf1N1B4_3005 0.41 -2.3 3,379,543 + Pf1N1B4_3005 0.41 +0.7 3,379,543 + Pf1N1B4_3005 0.41 -0.9 3,379,543 + Pf1N1B4_3005 0.41 -1.9 3,379,543 + Pf1N1B4_3005 0.41 -2.9 3,379,543 + Pf1N1B4_3005 0.41 -0.3 3,379,543 + -0.3 3,379,543 + Pf1N1B4_3005 0.41 -0.5 3,379,543 + Pf1N1B4_3005 0.41 -2.6 3,379,543 + Pf1N1B4_3005 0.41 +1.0 3,379,544 - Pf1N1B4_3005 0.42 -2.3 3,379,544 - Pf1N1B4_3005 0.42 -1.9 3,379,544 - Pf1N1B4_3005 0.42 +0.5 3,379,544 - Pf1N1B4_3005 0.42 -0.9 3,379,544 - Pf1N1B4_3005 0.42 -1.6 3,379,544 - Pf1N1B4_3005 0.42 -1.6 3,379,544 - Pf1N1B4_3005 0.42 +1.7 3,379,544 - Pf1N1B4_3005 0.42 -1.6 3,379,544 - Pf1N1B4_3005 0.42 +1.5 3,379,544 - Pf1N1B4_3005 0.42 +0.7 3,379,544 - Pf1N1B4_3005 0.42 -1.5 3,379,545 + Pf1N1B4_3005 0.42 -0.9 3,379,545 + -2.1 3,379,545 + Pf1N1B4_3005 0.42 -3.4 3,379,545 + Pf1N1B4_3005 0.42 -0.6 3,379,545 + Pf1N1B4_3005 0.42 -2.3 3,379,545 + Pf1N1B4_3005 0.42 -1.9 3,379,545 + Pf1N1B4_3005 0.42 -0.9 3,379,545 + Pf1N1B4_3005 0.42 +0.7 3,379,545 + Pf1N1B4_3005 0.42 -0.5 3,379,545 + Pf1N1B4_3005 0.42 -1.1 3,379,545 + +0.7 3,379,545 + Pf1N1B4_3005 0.42 -4.3 3,379,545 + Pf1N1B4_3005 0.42 -1.1 3,379,545 + Pf1N1B4_3005 0.42 +0.7 3,379,545 + -2.1 3,379,545 + Pf1N1B4_3005 0.42 -0.1 3,379,545 + Pf1N1B4_3005 0.42 -2.4 3,379,545 + Pf1N1B4_3005 0.42 -1.5 3,379,546 - Pf1N1B4_3005 0.42 -1.2 3,379,546 - Pf1N1B4_3005 0.42 +0.7 3,379,546 - Pf1N1B4_3005 0.42 -0.3 3,379,546 - Pf1N1B4_3005 0.42 +0.7 3,379,546 - Pf1N1B4_3005 0.42 -0.2 3,379,546 - Pf1N1B4_3005 0.42 +1.4 3,379,546 - Pf1N1B4_3005 0.42 +0.7 3,379,547 + Pf1N1B4_3005 0.42 -0.3 3,379,547 + Pf1N1B4_3005 0.42 +1.4 3,379,547 + Pf1N1B4_3005 0.42 -2.3 3,379,547 + Pf1N1B4_3005 0.42 +0.1 3,379,547 + Pf1N1B4_3005 0.42 +0.7 3,379,547 + Pf1N1B4_3005 0.42 -2.6 3,379,547 + Pf1N1B4_3005 0.42 -0.3 3,379,547 + Pf1N1B4_3005 0.42 -1.6 3,379,547 + Pf1N1B4_3005 0.42 -0.6 3,379,547 + Pf1N1B4_3005 0.42 -0.6 3,379,548 - Pf1N1B4_3005 0.42 +0.7 3,379,548 - Pf1N1B4_3005 0.42 +0.7 3,379,548 - Pf1N1B4_3005 0.42 +0.7 3,379,595 - Pf1N1B4_3005 0.46 +0.7 3,379,595 - Pf1N1B4_3005 0.46 -3.5 3,379,595 - Pf1N1B4_3005 0.46 -1.9 3,379,595 - Pf1N1B4_3005 0.46 +0.7 3,379,858 - Pf1N1B4_3005 0.71 -1.4 3,379,924 - Pf1N1B4_3005 0.77 +1.0 3,379,929 + Pf1N1B4_3005 0.78 +0.7 3,379,929 + Pf1N1B4_3005 0.78 -1.6 3,379,929 + Pf1N1B4_3005 0.78 -3.0 3,379,929 + Pf1N1B4_3005 0.78 -2.6 3,379,929 + Pf1N1B4_3005 0.78 -1.9 3,379,929 + Pf1N1B4_3005 0.78 -0.9 3,379,929 + Pf1N1B4_3005 0.78 -1.6 3,379,930 - Pf1N1B4_3005 0.78 -0.3 3,379,930 - Pf1N1B4_3005 0.78 -2.3 3,379,930 - Pf1N1B4_3005 0.78 +0.5 3,379,930 - Pf1N1B4_3005 0.78 -2.1 3,379,930 - Pf1N1B4_3005 0.78 -0.1
Or see this region's nucleotide sequence