Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5029 and Pf1N1B4_5030 are separated by 0 nucleotides Pf1N1B4_5030 and Pf1N1B4_5031 are separated by 3 nucleotides Pf1N1B4_5031 and Pf1N1B4_5032 are separated by 75 nucleotides
Pf1N1B4_5029 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 5,575,475 to 5,576,233
_5029
Pf1N1B4_5030 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 5,576,234 to 5,576,914
_5030
Pf1N1B4_5031 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 5,576,918 to 5,577,997
_5031
Pf1N1B4_5032 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 5,578,073 to 5,579,110
_5032
Position (kb) 5576
5577
5578 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5576.049 kb on - strand, within Pf1N1B4_5029 at 5576.138 kb on - strand, within Pf1N1B4_5029 at 5576.177 kb on + strand at 5576.231 kb on + strand at 5576.232 kb on - strand at 5576.426 kb on + strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand, within Pf1N1B4_5030 at 5576.427 kb on - strand at 5576.537 kb on + strand, within Pf1N1B4_5030 at 5576.730 kb on + strand, within Pf1N1B4_5030 at 5576.731 kb on - strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.733 kb on + strand, within Pf1N1B4_5030 at 5576.734 kb on - strand at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.789 kb on + strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.790 kb on - strand, within Pf1N1B4_5030 at 5576.890 kb on + strand at 5576.946 kb on - strand at 5577.375 kb on + strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.376 kb on - strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.462 kb on + strand, within Pf1N1B4_5031 at 5577.463 kb on - strand, within Pf1N1B4_5031 at 5577.650 kb on + strand, within Pf1N1B4_5031 at 5577.868 kb on - strand, within Pf1N1B4_5031 at 5578.037 kb on + strand at 5578.119 kb on - strand at 5578.376 kb on + strand, within Pf1N1B4_5032 at 5578.377 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.487 kb on - strand, within Pf1N1B4_5032 at 5578.530 kb on - strand, within Pf1N1B4_5032 at 5578.772 kb on + strand, within Pf1N1B4_5032 at 5578.776 kb on + strand, within Pf1N1B4_5032 at 5578.777 kb on - strand at 5578.964 kb on + strand, within Pf1N1B4_5032 at 5578.965 kb on - strand at 5578.965 kb on - strand, within Pf1N1B4_5032
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,576,049 - Pf1N1B4_5029 0.76 -2.4 5,576,138 - Pf1N1B4_5029 0.87 -3.4 5,576,177 + -4.4 5,576,231 + +0.4 5,576,232 - -2.6 5,576,426 + Pf1N1B4_5030 0.28 -2.9 5,576,427 - Pf1N1B4_5030 0.28 -0.4 5,576,427 - Pf1N1B4_5030 0.28 +0.4 5,576,427 - -1.2 5,576,537 + Pf1N1B4_5030 0.44 -2.7 5,576,730 + Pf1N1B4_5030 0.73 -3.1 5,576,731 - Pf1N1B4_5030 0.73 -5.4 5,576,733 + Pf1N1B4_5030 0.73 -1.9 5,576,733 + Pf1N1B4_5030 0.73 -3.9 5,576,734 - -0.0 5,576,789 + Pf1N1B4_5030 0.81 -1.9 5,576,789 + Pf1N1B4_5030 0.81 +0.4 5,576,790 - Pf1N1B4_5030 0.82 -5.1 5,576,790 - Pf1N1B4_5030 0.82 -1.2 5,576,890 + -0.0 5,576,946 - -4.2 5,577,375 + Pf1N1B4_5031 0.42 -2.9 5,577,376 - Pf1N1B4_5031 0.42 -3.1 5,577,376 - Pf1N1B4_5031 0.42 +0.4 5,577,376 - Pf1N1B4_5031 0.42 -3.0 5,577,462 + Pf1N1B4_5031 0.50 +0.4 5,577,462 + -3.7 5,577,462 + Pf1N1B4_5031 0.50 -3.4 5,577,462 + Pf1N1B4_5031 0.50 -2.6 5,577,463 - Pf1N1B4_5031 0.50 -3.7 5,577,650 + Pf1N1B4_5031 0.68 -3.9 5,577,868 - Pf1N1B4_5031 0.88 -1.6 5,578,037 + +0.4 5,578,119 - +0.4 5,578,376 + Pf1N1B4_5032 0.29 +0.4 5,578,377 - Pf1N1B4_5032 0.29 -3.6 5,578,487 - Pf1N1B4_5032 0.40 -0.1 5,578,487 - Pf1N1B4_5032 0.40 -1.2 5,578,530 - Pf1N1B4_5032 0.44 -2.4 5,578,772 + Pf1N1B4_5032 0.67 +0.4 5,578,776 + Pf1N1B4_5032 0.68 -0.6 5,578,777 - +0.4 5,578,964 + Pf1N1B4_5032 0.86 +0.7 5,578,965 - -0.6 5,578,965 - Pf1N1B4_5032 0.86 -0.6
Or see this region's nucleotide sequence