Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4814 and Pf1N1B4_4815 overlap by 4 nucleotides Pf1N1B4_4815 and Pf1N1B4_4816 are separated by 63 nucleotides
Pf1N1B4_4814 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 5,316,451 to 5,317,512
_4814
Pf1N1B4_4815 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 5,317,509 to 5,318,693
_4815
Pf1N1B4_4816 - Ferrichrome-iron receptor, at 5,318,757 to 5,321,153
_4816
Position (kb) 5317
5318
5319 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5316.536 kb on - strand at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.104 kb on - strand, within Pf1N1B4_4814 at 5317.104 kb on - strand at 5317.470 kb on - strand at 5317.492 kb on + strand at 5317.492 kb on + strand at 5317.493 kb on - strand at 5317.540 kb on + strand at 5317.666 kb on + strand, within Pf1N1B4_4815 at 5317.678 kb on + strand, within Pf1N1B4_4815 at 5318.201 kb on - strand, within Pf1N1B4_4815 at 5318.239 kb on + strand, within Pf1N1B4_4815 at 5318.482 kb on + strand, within Pf1N1B4_4815 at 5318.637 kb on + strand at 5318.839 kb on + strand at 5318.840 kb on - strand at 5318.971 kb on + strand at 5318.971 kb on + strand at 5318.986 kb on + strand at 5319.278 kb on + strand, within Pf1N1B4_4816 at 5319.295 kb on + strand, within Pf1N1B4_4816 at 5319.334 kb on + strand, within Pf1N1B4_4816 at 5319.334 kb on + strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.406 kb on + strand, within Pf1N1B4_4816 at 5319.406 kb on + strand, within Pf1N1B4_4816 at 5319.412 kb on + strand, within Pf1N1B4_4816 at 5319.511 kb on + strand at 5319.511 kb on + strand, within Pf1N1B4_4816 at 5319.511 kb on + strand, within Pf1N1B4_4816 at 5319.512 kb on - strand, within Pf1N1B4_4816 at 5319.512 kb on - strand at 5319.512 kb on - strand, within Pf1N1B4_4816 at 5319.676 kb on + strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,316,536 - +0.1 5,317,103 + Pf1N1B4_4814 0.61 -0.9 5,317,103 + Pf1N1B4_4814 0.61 -0.3 5,317,104 - Pf1N1B4_4814 0.61 -0.6 5,317,104 - -1.0 5,317,470 - -1.1 5,317,492 + +0.6 5,317,492 + -2.6 5,317,493 - +1.4 5,317,540 + +0.2 5,317,666 + Pf1N1B4_4815 0.13 +0.4 5,317,678 + Pf1N1B4_4815 0.14 -2.0 5,318,201 - Pf1N1B4_4815 0.58 -1.6 5,318,239 + Pf1N1B4_4815 0.62 -2.5 5,318,482 + Pf1N1B4_4815 0.82 -1.6 5,318,637 + +0.4 5,318,839 + -1.0 5,318,840 - -0.2 5,318,971 + +0.5 5,318,971 + +0.4 5,318,986 + -1.0 5,319,278 + Pf1N1B4_4816 0.22 +0.4 5,319,295 + Pf1N1B4_4816 0.22 -0.8 5,319,334 + Pf1N1B4_4816 0.24 +0.4 5,319,334 + Pf1N1B4_4816 0.24 -1.6 5,319,335 - Pf1N1B4_4816 0.24 -1.9 5,319,335 - Pf1N1B4_4816 0.24 -1.2 5,319,335 - Pf1N1B4_4816 0.24 -1.1 5,319,335 - Pf1N1B4_4816 0.24 +0.4 5,319,335 - -0.9 5,319,335 - Pf1N1B4_4816 0.24 +0.4 5,319,406 + Pf1N1B4_4816 0.27 +0.6 5,319,406 + Pf1N1B4_4816 0.27 -2.2 5,319,412 + Pf1N1B4_4816 0.27 +1.1 5,319,511 + -2.0 5,319,511 + Pf1N1B4_4816 0.31 +0.0 5,319,511 + Pf1N1B4_4816 0.31 -1.2 5,319,512 - Pf1N1B4_4816 0.31 +0.4 5,319,512 - -0.6 5,319,512 - Pf1N1B4_4816 0.31 +0.4 5,319,676 + Pf1N1B4_4816 0.38 +0.0 5,319,677 - Pf1N1B4_4816 0.38 +1.0 5,319,677 - Pf1N1B4_4816 0.38 -3.1 5,319,677 - Pf1N1B4_4816 0.38 -3.9 5,319,677 - Pf1N1B4_4816 0.38 -0.7 5,319,677 - Pf1N1B4_4816 0.38 -0.8
Or see this region's nucleotide sequence