Experiment: N2E2wt with N1B4m denitrifying repA 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4738 and Pf1N1B4_4739 overlap by 4 nucleotides Pf1N1B4_4739 and Pf1N1B4_4740 are separated by 231 nucleotides Pf1N1B4_4740 and Pf1N1B4_4741 are separated by 292 nucleotides 
        Pf1N1B4_4738 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 5,235,500 to 5,236,024 
        _4738 
         
        
        Pf1N1B4_4739 - Ribosyl nicotinamide transporter, PnuC-like, at 5,236,021 to 5,236,584 
        _4739 
         
        
        Pf1N1B4_4740 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,236,816 to 5,238,450 
        _4740 
         
        
        Pf1N1B4_4741 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 5,238,743 to 5,239,573 
        _4741 
         Position (kb) 5236 
5237 
5238 
5239 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 
2 at 5235.914 kb on - strand, within Pf1N1B4_4738 at 5235.927 kb on + strand, within Pf1N1B4_4738 at 5236.109 kb on + strand, within Pf1N1B4_4739 at 5236.109 kb on + strand, within Pf1N1B4_4739 at 5236.122 kb on - strand, within Pf1N1B4_4739 at 5236.122 kb on - strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.400 kb on + strand, within Pf1N1B4_4739 at 5236.401 kb on - strand, within Pf1N1B4_4739 at 5236.418 kb on + strand, within Pf1N1B4_4739 at 5236.419 kb on - strand, within Pf1N1B4_4739 at 5236.473 kb on + strand, within Pf1N1B4_4739 at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.474 kb on - strand at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.474 kb on - strand, within Pf1N1B4_4739 at 5236.475 kb on + strand, within Pf1N1B4_4739 at 5236.476 kb on - strand, within Pf1N1B4_4739 at 5236.756 kb on + strand at 5237.184 kb on + strand, within Pf1N1B4_4740 at 5237.513 kb on + strand, within Pf1N1B4_4740 at 5237.919 kb on - strand, within Pf1N1B4_4740 at 5237.919 kb on - strand, within Pf1N1B4_4740 at 5237.966 kb on + strand at 5237.966 kb on + strand, within Pf1N1B4_4740 at 5238.379 kb on + strand at 5238.379 kb on + strand at 5238.480 kb on + strand at 5238.541 kb on + strand at 5238.687 kb on - strand at 5238.687 kb on - strand at 5238.693 kb on + strand at 5238.693 kb on + strand at 5238.836 kb on - strand, within Pf1N1B4_4741 at 5238.883 kb on + strand, within Pf1N1B4_4741 at 5238.883 kb on + strand at 5238.886 kb on + strand, within Pf1N1B4_4741 at 5238.886 kb on + strand at 5239.072 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand, within Pf1N1B4_4741 at 5239.174 kb on + strand at 5239.346 kb on + strand, within Pf1N1B4_4741 at 5239.355 kb on + strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction N2E2wt with N1B4m denitrifying repA remove 5,235,914 -  Pf1N1B4_4738 0.79  -2.0 5,235,927 +  Pf1N1B4_4738 0.81  -0.2 5,236,109 +  Pf1N1B4_4739 0.16  -0.3 5,236,109 +  Pf1N1B4_4739 0.16  +0.5 5,236,122 -  Pf1N1B4_4739 0.18  -2.6 5,236,122 -  Pf1N1B4_4739 0.18  +1.0 5,236,400 +  Pf1N1B4_4739 0.67  -1.2 5,236,400 +  Pf1N1B4_4739 0.67  -0.4 5,236,400 +  Pf1N1B4_4739 0.67  +1.8 5,236,401 -  Pf1N1B4_4739 0.67  +0.0 5,236,418 +  Pf1N1B4_4739 0.70  +0.4 5,236,419 -  Pf1N1B4_4739 0.71  -0.8 5,236,473 +  Pf1N1B4_4739 0.80  -0.6 5,236,474 -  Pf1N1B4_4739 0.80  +0.4 5,236,474 -  +0.4 5,236,474 -  Pf1N1B4_4739 0.80  -0.4 5,236,474 -  Pf1N1B4_4739 0.80  +0.2 5,236,475 +  Pf1N1B4_4739 0.80  -4.3 5,236,476 -  Pf1N1B4_4739 0.81  -3.4 5,236,756 +  -2.0 5,237,184 +  Pf1N1B4_4740 0.23  +0.4 5,237,513 +  Pf1N1B4_4740 0.43  -2.4 5,237,919 -  Pf1N1B4_4740 0.67  -0.6 5,237,919 -  Pf1N1B4_4740 0.67  +0.4 5,237,966 +  -0.8 5,237,966 +  Pf1N1B4_4740 0.70  -2.9 5,238,379 +  -3.2 5,238,379 +  -1.9 5,238,480 +  +0.4 5,238,541 +  +0.4 5,238,687 -  -0.6 5,238,687 -  -1.3 5,238,693 +  -2.1 5,238,693 +  -0.8 5,238,836 -  Pf1N1B4_4741 0.11  -1.2 5,238,883 +  Pf1N1B4_4741 0.17  +0.4 5,238,883 +  -0.7 5,238,886 +  Pf1N1B4_4741 0.17  +0.4 5,238,886 +  -2.8 5,239,072 +  Pf1N1B4_4741 0.40  +1.1 5,239,174 +  Pf1N1B4_4741 0.52  -3.2 5,239,174 +  Pf1N1B4_4741 0.52  -0.1 5,239,174 +  +0.4 5,239,346 +  Pf1N1B4_4741 0.73  -1.6 5,239,355 +  -1.0 
Or see this region's nucleotide sequence