Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4732 and Pf1N1B4_4733 are separated by 157 nucleotides Pf1N1B4_4733 and Pf1N1B4_4734 are separated by 32 nucleotides Pf1N1B4_4734 and Pf1N1B4_4735 are separated by 62 nucleotides
Pf1N1B4_4732 - Fap amyloid fiber secretin, at 5,229,825 to 5,230,994
_4732
Pf1N1B4_4733 - Fap system putative outer membrane protein, at 5,231,152 to 5,231,889
_4733
Pf1N1B4_4734 - Fap protein with C39 domain, at 5,231,922 to 5,232,602
_4734
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Position (kb) 5231
5232
5233 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5230.947 kb on + strand at 5231.008 kb on - strand at 5231.038 kb on + strand at 5231.038 kb on + strand at 5231.038 kb on + strand at 5231.039 kb on - strand at 5231.039 kb on - strand at 5231.039 kb on - strand at 5231.078 kb on + strand at 5231.078 kb on + strand at 5231.127 kb on + strand at 5231.128 kb on - strand at 5231.148 kb on + strand at 5231.148 kb on + strand at 5231.148 kb on + strand at 5231.371 kb on - strand, within Pf1N1B4_4733 at 5231.504 kb on + strand at 5231.514 kb on + strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.643 kb on - strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5231.698 kb on + strand, within Pf1N1B4_4733 at 5232.145 kb on + strand at 5232.145 kb on + strand, within Pf1N1B4_4734 at 5232.146 kb on - strand, within Pf1N1B4_4734 at 5232.213 kb on + strand, within Pf1N1B4_4734 at 5232.214 kb on - strand, within Pf1N1B4_4734 at 5232.397 kb on + strand, within Pf1N1B4_4734 at 5232.411 kb on + strand at 5232.476 kb on + strand, within Pf1N1B4_4734 at 5232.487 kb on - strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.505 kb on + strand, within Pf1N1B4_4734 at 5232.572 kb on - strand at 5232.572 kb on - strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.831 kb on + strand at 5232.831 kb on + strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.832 kb on - strand, within Pf1N1B4_4735 at 5232.867 kb on + strand at 5232.868 kb on - strand, within Pf1N1B4_4735 at 5232.904 kb on + strand, within Pf1N1B4_4735 at 5232.953 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.349 kb on - strand, within Pf1N1B4_4735
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 5,230,947 + -1.1 5,231,008 - -3.4 5,231,038 + +0.3 5,231,038 + -1.5 5,231,038 + -0.3 5,231,039 - -0.0 5,231,039 - -1.2 5,231,039 - -1.6 5,231,078 + -0.6 5,231,078 + -1.8 5,231,127 + +2.2 5,231,128 - +0.4 5,231,148 + -1.2 5,231,148 + -1.9 5,231,148 + -1.9 5,231,371 - Pf1N1B4_4733 0.30 +0.4 5,231,504 + +0.4 5,231,514 + Pf1N1B4_4733 0.49 -2.6 5,231,643 - Pf1N1B4_4733 0.67 -2.3 5,231,643 - Pf1N1B4_4733 0.67 +0.4 5,231,643 - Pf1N1B4_4733 0.67 -3.1 5,231,698 + Pf1N1B4_4733 0.74 -2.4 5,231,698 + Pf1N1B4_4733 0.74 +0.4 5,232,145 + -0.7 5,232,145 + Pf1N1B4_4734 0.33 -0.8 5,232,146 - Pf1N1B4_4734 0.33 -0.3 5,232,213 + Pf1N1B4_4734 0.43 +0.4 5,232,214 - Pf1N1B4_4734 0.43 -1.9 5,232,397 + Pf1N1B4_4734 0.70 +0.4 5,232,411 + -2.4 5,232,476 + Pf1N1B4_4734 0.81 -2.9 5,232,487 - Pf1N1B4_4734 0.83 -2.2 5,232,505 + Pf1N1B4_4734 0.86 -2.4 5,232,505 + Pf1N1B4_4734 0.86 -1.2 5,232,572 - -0.0 5,232,572 - -1.6 5,232,831 + Pf1N1B4_4735 0.13 -1.2 5,232,831 + +0.4 5,232,831 + Pf1N1B4_4735 0.13 -0.5 5,232,832 - Pf1N1B4_4735 0.13 +3.0 5,232,832 - Pf1N1B4_4735 0.13 -1.9 5,232,832 - Pf1N1B4_4735 0.13 -0.6 5,232,867 + +1.4 5,232,868 - Pf1N1B4_4735 0.16 +1.0 5,232,904 + Pf1N1B4_4735 0.19 -0.3 5,232,953 - Pf1N1B4_4735 0.22 -0.4 5,233,035 - Pf1N1B4_4735 0.29 +0.4 5,233,035 - Pf1N1B4_4735 0.29 -3.6 5,233,349 - Pf1N1B4_4735 0.53 -0.3
Or see this region's nucleotide sequence