Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4273 and Pf1N1B4_4274 are separated by 311 nucleotides Pf1N1B4_4274 and Pf1N1B4_4275 are separated by 172 nucleotides
Pf1N1B4_4273 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 4,737,166 to 4,738,086
_4273
Pf1N1B4_4274 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 4,738,398 to 4,740,335
_4274
Pf1N1B4_4275 - Inosose dehydratase (EC 4.2.1.44), at 4,740,508 to 4,741,401
_4275
Position (kb)
4738
4739
4740
4741 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.588 kb on - strand, within Pf1N1B4_4273 at 4737.761 kb on + strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.941 kb on + strand, within Pf1N1B4_4273 at 4737.941 kb on + strand at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.980 kb on + strand, within Pf1N1B4_4273 at 4737.981 kb on - strand, within Pf1N1B4_4273 at 4738.087 kb on - strand at 4738.144 kb on + strand at 4738.144 kb on + strand at 4738.145 kb on - strand at 4738.145 kb on - strand at 4738.456 kb on + strand at 4738.470 kb on + strand at 4738.521 kb on + strand at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4739.209 kb on - strand at 4739.401 kb on + strand, within Pf1N1B4_4274 at 4739.401 kb on + strand, within Pf1N1B4_4274 at 4739.402 kb on - strand, within Pf1N1B4_4274 at 4739.402 kb on - strand, within Pf1N1B4_4274 at 4739.898 kb on + strand, within Pf1N1B4_4274 at 4739.899 kb on - strand, within Pf1N1B4_4274 at 4740.339 kb on - strand at 4740.350 kb on - strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.425 kb on - strand at 4740.494 kb on + strand at 4740.691 kb on + strand, within Pf1N1B4_4275 at 4740.786 kb on - strand, within Pf1N1B4_4275 at 4740.965 kb on - strand, within Pf1N1B4_4275 at 4741.069 kb on + strand, within Pf1N1B4_4275 at 4741.069 kb on + strand, within Pf1N1B4_4275 at 4741.070 kb on - strand, within Pf1N1B4_4275 at 4741.070 kb on - strand at 4741.070 kb on - strand, within Pf1N1B4_4275
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 4,737,539 + Pf1N1B4_4273 0.40 +0.3 4,737,539 + Pf1N1B4_4273 0.40 +0.5 4,737,587 + Pf1N1B4_4273 0.46 +0.5 4,737,587 + -0.5 4,737,587 + Pf1N1B4_4273 0.46 -0.5 4,737,587 + -1.6 4,737,588 - Pf1N1B4_4273 0.46 +0.5 4,737,761 + Pf1N1B4_4273 0.65 +2.1 4,737,875 - Pf1N1B4_4273 0.77 -0.5 4,737,875 - Pf1N1B4_4273 0.77 -0.5 4,737,941 + Pf1N1B4_4273 0.84 -1.1 4,737,941 + -0.7 4,737,942 - Pf1N1B4_4273 0.84 -2.6 4,737,942 - Pf1N1B4_4273 0.84 +0.4 4,737,942 - Pf1N1B4_4273 0.84 +0.5 4,737,980 + Pf1N1B4_4273 0.88 -3.6 4,737,981 - Pf1N1B4_4273 0.88 -0.1 4,738,087 - -1.2 4,738,144 + -1.9 4,738,144 + +0.5 4,738,145 - +1.5 4,738,145 - +0.5 4,738,456 + -0.5 4,738,470 + +0.5 4,738,521 + +0.2 4,738,884 + Pf1N1B4_4274 0.25 +0.5 4,738,884 + Pf1N1B4_4274 0.25 +0.5 4,738,884 + Pf1N1B4_4274 0.25 -1.7 4,739,209 - -2.7 4,739,401 + Pf1N1B4_4274 0.52 -1.8 4,739,401 + Pf1N1B4_4274 0.52 -1.4 4,739,402 - Pf1N1B4_4274 0.52 -0.4 4,739,402 - Pf1N1B4_4274 0.52 -2.1 4,739,898 + Pf1N1B4_4274 0.77 -2.7 4,739,899 - Pf1N1B4_4274 0.77 +0.5 4,740,339 - +0.2 4,740,350 - +0.3 4,740,411 + -3.1 4,740,411 + +0.2 4,740,411 + +0.5 4,740,411 + -1.8 4,740,411 + -2.0 4,740,425 - -1.8 4,740,494 + +0.6 4,740,691 + Pf1N1B4_4275 0.20 -2.8 4,740,786 - Pf1N1B4_4275 0.31 +0.5 4,740,965 - Pf1N1B4_4275 0.51 -0.2 4,741,069 + Pf1N1B4_4275 0.63 -2.7 4,741,069 + Pf1N1B4_4275 0.63 -2.7 4,741,070 - Pf1N1B4_4275 0.63 -0.1 4,741,070 - +0.5 4,741,070 - Pf1N1B4_4275 0.63 +0.7
Or see this region's nucleotide sequence