Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4157 and Pf1N1B4_4158 are separated by 29 nucleotides Pf1N1B4_4158 and Pf1N1B4_4159 overlap by 1 nucleotides Pf1N1B4_4159 and Pf1N1B4_4160 overlap by 4 nucleotides Pf1N1B4_4160 and Pf1N1B4_4161 overlap by 4 nucleotides
Pf1N1B4_4157 - Probable signal peptide protein, at 4,602,069 to 4,602,860
_4157
Pf1N1B4_4158 - FIG00955149: hypothetical protein, at 4,602,890 to 4,604,377
_4158
Pf1N1B4_4159 - FIG00955149: hypothetical protein, at 4,604,377 to 4,604,604
_4159
Pf1N1B4_4160 - FIG00956113: hypothetical protein, at 4,604,601 to 4,605,239
_4160
Pf1N1B4_4161 - UPF0192 protein all5100 precursor, at 4,605,236 to 4,609,810
_4161
Position (kb)
4602
4603
4604
4605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4601.920 kb on + strand at 4601.920 kb on + strand at 4601.920 kb on + strand at 4601.921 kb on - strand at 4601.922 kb on + strand at 4601.922 kb on + strand at 4601.938 kb on - strand at 4602.006 kb on + strand at 4602.012 kb on - strand at 4602.012 kb on - strand at 4602.015 kb on + strand at 4602.066 kb on + strand at 4602.302 kb on + strand, within Pf1N1B4_4157 at 4602.334 kb on + strand, within Pf1N1B4_4157 at 4602.334 kb on + strand, within Pf1N1B4_4157 at 4602.334 kb on + strand at 4602.334 kb on + strand, within Pf1N1B4_4157 at 4602.335 kb on - strand at 4602.335 kb on - strand, within Pf1N1B4_4157 at 4602.335 kb on - strand, within Pf1N1B4_4157 at 4602.336 kb on + strand, within Pf1N1B4_4157 at 4602.445 kb on - strand, within Pf1N1B4_4157 at 4602.661 kb on - strand, within Pf1N1B4_4157 at 4602.661 kb on - strand, within Pf1N1B4_4157 at 4602.669 kb on + strand, within Pf1N1B4_4157 at 4602.678 kb on + strand, within Pf1N1B4_4157 at 4602.679 kb on - strand, within Pf1N1B4_4157 at 4602.679 kb on - strand, within Pf1N1B4_4157 at 4602.679 kb on - strand, within Pf1N1B4_4157 at 4602.707 kb on + strand, within Pf1N1B4_4157 at 4602.707 kb on + strand, within Pf1N1B4_4157 at 4602.855 kb on + strand at 4602.855 kb on + strand at 4602.856 kb on - strand at 4602.856 kb on - strand at 4602.858 kb on + strand at 4602.905 kb on - strand at 4603.175 kb on + strand, within Pf1N1B4_4158 at 4603.175 kb on + strand, within Pf1N1B4_4158 at 4603.176 kb on - strand, within Pf1N1B4_4158 at 4603.176 kb on - strand, within Pf1N1B4_4158 at 4603.176 kb on - strand, within Pf1N1B4_4158 at 4603.176 kb on - strand, within Pf1N1B4_4158 at 4603.233 kb on - strand, within Pf1N1B4_4158 at 4603.233 kb on - strand, within Pf1N1B4_4158 at 4603.406 kb on - strand, within Pf1N1B4_4158 at 4603.472 kb on + strand, within Pf1N1B4_4158 at 4603.473 kb on - strand, within Pf1N1B4_4158 at 4603.473 kb on - strand, within Pf1N1B4_4158 at 4604.003 kb on + strand, within Pf1N1B4_4158 at 4604.029 kb on - strand, within Pf1N1B4_4158 at 4604.029 kb on - strand, within Pf1N1B4_4158 at 4604.244 kb on + strand at 4604.386 kb on + strand at 4604.387 kb on - strand at 4604.468 kb on - strand, within Pf1N1B4_4159 at 4604.468 kb on - strand, within Pf1N1B4_4159 at 4604.486 kb on - strand, within Pf1N1B4_4159 at 4604.527 kb on + strand, within Pf1N1B4_4159 at 4604.528 kb on - strand, within Pf1N1B4_4159 at 4604.528 kb on - strand, within Pf1N1B4_4159 at 4604.950 kb on - strand, within Pf1N1B4_4160
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 4,601,920 + +0.2 4,601,920 + +0.5 4,601,920 + -1.7 4,601,921 - +0.5 4,601,922 + -0.5 4,601,922 + +0.5 4,601,938 - -1.6 4,602,006 + -1.0 4,602,012 - -0.5 4,602,012 - +0.5 4,602,015 + -1.0 4,602,066 + +0.5 4,602,302 + Pf1N1B4_4157 0.29 -0.5 4,602,334 + Pf1N1B4_4157 0.33 +0.5 4,602,334 + Pf1N1B4_4157 0.33 -1.5 4,602,334 + -2.5 4,602,334 + Pf1N1B4_4157 0.33 +0.5 4,602,335 - +0.0 4,602,335 - Pf1N1B4_4157 0.34 +0.5 4,602,335 - Pf1N1B4_4157 0.34 -0.1 4,602,336 + Pf1N1B4_4157 0.34 -0.4 4,602,445 - Pf1N1B4_4157 0.47 +0.5 4,602,661 - Pf1N1B4_4157 0.75 -1.0 4,602,661 - Pf1N1B4_4157 0.75 -2.9 4,602,669 + Pf1N1B4_4157 0.76 +0.5 4,602,678 + Pf1N1B4_4157 0.77 -0.6 4,602,679 - Pf1N1B4_4157 0.77 -2.8 4,602,679 - Pf1N1B4_4157 0.77 -1.5 4,602,679 - Pf1N1B4_4157 0.77 -0.5 4,602,707 + Pf1N1B4_4157 0.81 +0.5 4,602,707 + Pf1N1B4_4157 0.81 -1.3 4,602,855 + +0.5 4,602,855 + -4.2 4,602,856 - +0.5 4,602,856 - -0.3 4,602,858 + -0.1 4,602,905 - -3.0 4,603,175 + Pf1N1B4_4158 0.19 +0.2 4,603,175 + Pf1N1B4_4158 0.19 -0.6 4,603,176 - Pf1N1B4_4158 0.19 -0.7 4,603,176 - Pf1N1B4_4158 0.19 +0.1 4,603,176 - Pf1N1B4_4158 0.19 -1.0 4,603,176 - Pf1N1B4_4158 0.19 -0.1 4,603,233 - Pf1N1B4_4158 0.23 +0.5 4,603,233 - Pf1N1B4_4158 0.23 -0.0 4,603,406 - Pf1N1B4_4158 0.35 +0.0 4,603,472 + Pf1N1B4_4158 0.39 +0.6 4,603,473 - Pf1N1B4_4158 0.39 -3.3 4,603,473 - Pf1N1B4_4158 0.39 +0.2 4,604,003 + Pf1N1B4_4158 0.75 -1.2 4,604,029 - Pf1N1B4_4158 0.77 -0.0 4,604,029 - Pf1N1B4_4158 0.77 +0.5 4,604,244 + -2.9 4,604,386 + -0.3 4,604,387 - +0.9 4,604,468 - Pf1N1B4_4159 0.40 -3.5 4,604,468 - Pf1N1B4_4159 0.40 -0.1 4,604,486 - Pf1N1B4_4159 0.48 -0.5 4,604,527 + Pf1N1B4_4159 0.66 -0.9 4,604,528 - Pf1N1B4_4159 0.66 +0.5 4,604,528 - Pf1N1B4_4159 0.66 +0.1 4,604,950 - Pf1N1B4_4160 0.55 +0.1
Or see this region's nucleotide sequence