Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4158

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4157 and Pf1N1B4_4158 are separated by 29 nucleotidesPf1N1B4_4158 and Pf1N1B4_4159 overlap by 1 nucleotidesPf1N1B4_4159 and Pf1N1B4_4160 overlap by 4 nucleotidesPf1N1B4_4160 and Pf1N1B4_4161 overlap by 4 nucleotides Pf1N1B4_4157 - Probable signal peptide protein, at 4,602,069 to 4,602,860 _4157 Pf1N1B4_4158 - FIG00955149: hypothetical protein, at 4,602,890 to 4,604,377 _4158 Pf1N1B4_4159 - FIG00955149: hypothetical protein, at 4,604,377 to 4,604,604 _4159 Pf1N1B4_4160 - FIG00956113: hypothetical protein, at 4,604,601 to 4,605,239 _4160 Pf1N1B4_4161 - UPF0192 protein all5100 precursor, at 4,605,236 to 4,609,810 _4161 Position (kb) 4602 4603 4604 4605Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4601.920 kb on + strandat 4601.920 kb on + strandat 4601.920 kb on + strandat 4601.921 kb on - strandat 4601.922 kb on + strandat 4601.922 kb on + strandat 4601.938 kb on - strandat 4602.006 kb on + strandat 4602.012 kb on - strandat 4602.012 kb on - strandat 4602.015 kb on + strandat 4602.066 kb on + strandat 4602.302 kb on + strand, within Pf1N1B4_4157at 4602.334 kb on + strand, within Pf1N1B4_4157at 4602.334 kb on + strand, within Pf1N1B4_4157at 4602.334 kb on + strandat 4602.334 kb on + strand, within Pf1N1B4_4157at 4602.335 kb on - strandat 4602.335 kb on - strand, within Pf1N1B4_4157at 4602.335 kb on - strand, within Pf1N1B4_4157at 4602.336 kb on + strand, within Pf1N1B4_4157at 4602.445 kb on - strand, within Pf1N1B4_4157at 4602.661 kb on - strand, within Pf1N1B4_4157at 4602.661 kb on - strand, within Pf1N1B4_4157at 4602.669 kb on + strand, within Pf1N1B4_4157at 4602.678 kb on + strand, within Pf1N1B4_4157at 4602.679 kb on - strand, within Pf1N1B4_4157at 4602.679 kb on - strand, within Pf1N1B4_4157at 4602.679 kb on - strand, within Pf1N1B4_4157at 4602.707 kb on + strand, within Pf1N1B4_4157at 4602.707 kb on + strand, within Pf1N1B4_4157at 4602.855 kb on + strandat 4602.855 kb on + strandat 4602.856 kb on - strandat 4602.856 kb on - strandat 4602.858 kb on + strandat 4602.905 kb on - strandat 4603.175 kb on + strand, within Pf1N1B4_4158at 4603.175 kb on + strand, within Pf1N1B4_4158at 4603.176 kb on - strand, within Pf1N1B4_4158at 4603.176 kb on - strand, within Pf1N1B4_4158at 4603.176 kb on - strand, within Pf1N1B4_4158at 4603.176 kb on - strand, within Pf1N1B4_4158at 4603.233 kb on - strand, within Pf1N1B4_4158at 4603.233 kb on - strand, within Pf1N1B4_4158at 4603.406 kb on - strand, within Pf1N1B4_4158at 4603.472 kb on + strand, within Pf1N1B4_4158at 4603.473 kb on - strand, within Pf1N1B4_4158at 4603.473 kb on - strand, within Pf1N1B4_4158at 4604.003 kb on + strand, within Pf1N1B4_4158at 4604.029 kb on - strand, within Pf1N1B4_4158at 4604.029 kb on - strand, within Pf1N1B4_4158at 4604.244 kb on + strandat 4604.386 kb on + strandat 4604.387 kb on - strandat 4604.468 kb on - strand, within Pf1N1B4_4159at 4604.468 kb on - strand, within Pf1N1B4_4159at 4604.486 kb on - strand, within Pf1N1B4_4159at 4604.527 kb on + strand, within Pf1N1B4_4159at 4604.528 kb on - strand, within Pf1N1B4_4159at 4604.528 kb on - strand, within Pf1N1B4_4159at 4604.950 kb on - strand, within Pf1N1B4_4160

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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4,601,920 + +0.2
4,601,920 + +0.5
4,601,920 + -1.7
4,601,921 - +0.5
4,601,922 + -0.5
4,601,922 + +0.5
4,601,938 - -1.6
4,602,006 + -1.0
4,602,012 - -0.5
4,602,012 - +0.5
4,602,015 + -1.0
4,602,066 + +0.5
4,602,302 + Pf1N1B4_4157 0.29 -0.5
4,602,334 + Pf1N1B4_4157 0.33 +0.5
4,602,334 + Pf1N1B4_4157 0.33 -1.5
4,602,334 + -2.5
4,602,334 + Pf1N1B4_4157 0.33 +0.5
4,602,335 - +0.0
4,602,335 - Pf1N1B4_4157 0.34 +0.5
4,602,335 - Pf1N1B4_4157 0.34 -0.1
4,602,336 + Pf1N1B4_4157 0.34 -0.4
4,602,445 - Pf1N1B4_4157 0.47 +0.5
4,602,661 - Pf1N1B4_4157 0.75 -1.0
4,602,661 - Pf1N1B4_4157 0.75 -2.9
4,602,669 + Pf1N1B4_4157 0.76 +0.5
4,602,678 + Pf1N1B4_4157 0.77 -0.6
4,602,679 - Pf1N1B4_4157 0.77 -2.8
4,602,679 - Pf1N1B4_4157 0.77 -1.5
4,602,679 - Pf1N1B4_4157 0.77 -0.5
4,602,707 + Pf1N1B4_4157 0.81 +0.5
4,602,707 + Pf1N1B4_4157 0.81 -1.3
4,602,855 + +0.5
4,602,855 + -4.2
4,602,856 - +0.5
4,602,856 - -0.3
4,602,858 + -0.1
4,602,905 - -3.0
4,603,175 + Pf1N1B4_4158 0.19 +0.2
4,603,175 + Pf1N1B4_4158 0.19 -0.6
4,603,176 - Pf1N1B4_4158 0.19 -0.7
4,603,176 - Pf1N1B4_4158 0.19 +0.1
4,603,176 - Pf1N1B4_4158 0.19 -1.0
4,603,176 - Pf1N1B4_4158 0.19 -0.1
4,603,233 - Pf1N1B4_4158 0.23 +0.5
4,603,233 - Pf1N1B4_4158 0.23 -0.0
4,603,406 - Pf1N1B4_4158 0.35 +0.0
4,603,472 + Pf1N1B4_4158 0.39 +0.6
4,603,473 - Pf1N1B4_4158 0.39 -3.3
4,603,473 - Pf1N1B4_4158 0.39 +0.2
4,604,003 + Pf1N1B4_4158 0.75 -1.2
4,604,029 - Pf1N1B4_4158 0.77 -0.0
4,604,029 - Pf1N1B4_4158 0.77 +0.5
4,604,244 + -2.9
4,604,386 + -0.3
4,604,387 - +0.9
4,604,468 - Pf1N1B4_4159 0.40 -3.5
4,604,468 - Pf1N1B4_4159 0.40 -0.1
4,604,486 - Pf1N1B4_4159 0.48 -0.5
4,604,527 + Pf1N1B4_4159 0.66 -0.9
4,604,528 - Pf1N1B4_4159 0.66 +0.5
4,604,528 - Pf1N1B4_4159 0.66 +0.1
4,604,950 - Pf1N1B4_4160 0.55 +0.1

Or see this region's nucleotide sequence