Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_414 and Pf1N1B4_415 are separated by 242 nucleotides Pf1N1B4_415 and Pf1N1B4_416 are separated by 57 nucleotides Pf1N1B4_416 and Pf1N1B4_417 are separated by 16 nucleotides
Pf1N1B4_414 - transcriptional regulator, at 483,634 to 484,350
_414
Pf1N1B4_415 - Lipase precursor (EC 3.1.1.3), at 484,593 to 485,471
_415
Pf1N1B4_416 - FIG003620: Proteophosphoglycan precursor (Fragment), at 485,529 to 486,089
_416
Pf1N1B4_417 - lipoprotein, putative, at 486,106 to 486,711
_417
Position (kb)
484
485
486 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 483.635 kb on + strand at 483.636 kb on - strand at 483.926 kb on + strand, within Pf1N1B4_414 at 483.965 kb on + strand, within Pf1N1B4_414 at 483.965 kb on + strand, within Pf1N1B4_414 at 483.966 kb on - strand, within Pf1N1B4_414 at 483.966 kb on - strand at 483.966 kb on - strand at 483.966 kb on - strand, within Pf1N1B4_414 at 484.148 kb on - strand, within Pf1N1B4_414 at 484.523 kb on - strand at 484.523 kb on - strand at 484.527 kb on + strand at 484.527 kb on + strand at 484.527 kb on + strand at 484.528 kb on - strand at 484.588 kb on + strand at 484.588 kb on + strand at 484.589 kb on - strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.593 kb on - strand at 484.617 kb on - strand at 484.624 kb on - strand at 484.624 kb on - strand at 484.680 kb on + strand at 484.870 kb on - strand, within Pf1N1B4_415 at 484.874 kb on - strand at 484.996 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.125 kb on - strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.187 kb on + strand at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.191 kb on - strand at 485.209 kb on + strand, within Pf1N1B4_415 at 485.258 kb on + strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand at 485.319 kb on + strand, within Pf1N1B4_415 at 485.430 kb on + strand at 485.431 kb on - strand at 485.460 kb on - strand at 485.460 kb on - strand at 485.469 kb on + strand at 485.470 kb on - strand at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.630 kb on - strand, within Pf1N1B4_416 at 485.680 kb on + strand, within Pf1N1B4_416 at 485.681 kb on - strand, within Pf1N1B4_416 at 485.724 kb on - strand, within Pf1N1B4_416 at 485.724 kb on - strand, within Pf1N1B4_416 at 485.878 kb on + strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand, within Pf1N1B4_416 at 485.879 kb on - strand at 485.953 kb on + strand, within Pf1N1B4_416 at 485.953 kb on + strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.954 kb on - strand, within Pf1N1B4_416 at 485.956 kb on + strand at 485.956 kb on + strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 485.957 kb on - strand, within Pf1N1B4_416 at 486.107 kb on + strand at 486.108 kb on - strand at 486.141 kb on - strand at 486.196 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.216 kb on + strand, within Pf1N1B4_417 at 486.217 kb on - strand, within Pf1N1B4_417 at 486.226 kb on - strand, within Pf1N1B4_417 at 486.236 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand, within Pf1N1B4_417 at 486.365 kb on + strand at 486.365 kb on + strand, within Pf1N1B4_417 at 486.374 kb on + strand, within Pf1N1B4_417 at 486.455 kb on + strand, within Pf1N1B4_417 at 486.455 kb on + strand, within Pf1N1B4_417 at 486.456 kb on - strand, within Pf1N1B4_417
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 483,635 + +0.0 483,636 - +0.1 483,926 + Pf1N1B4_414 0.41 -2.4 483,965 + Pf1N1B4_414 0.46 +0.1 483,965 + Pf1N1B4_414 0.46 +0.7 483,966 - Pf1N1B4_414 0.46 -2.3 483,966 - -1.6 483,966 - +0.7 483,966 - Pf1N1B4_414 0.46 +0.5 484,148 - Pf1N1B4_414 0.72 +0.7 484,523 - -0.2 484,523 - -1.3 484,527 + -3.4 484,527 + -1.2 484,527 + +0.0 484,528 - -2.1 484,588 + -1.9 484,588 + +1.1 484,589 - -0.9 484,592 + -0.1 484,592 + -0.7 484,592 + +0.7 484,592 + +0.0 484,592 + +0.4 484,593 - +0.7 484,617 - -3.3 484,624 - +0.7 484,624 - -1.6 484,680 + +1.9 484,870 - Pf1N1B4_415 0.32 -1.3 484,874 - -2.6 484,996 + Pf1N1B4_415 0.46 -0.3 485,124 + Pf1N1B4_415 0.60 +0.0 485,124 + Pf1N1B4_415 0.60 -3.4 485,125 - Pf1N1B4_415 0.61 -3.6 485,184 + Pf1N1B4_415 0.67 +0.5 485,184 + +0.7 485,184 + Pf1N1B4_415 0.67 +0.5 485,184 + Pf1N1B4_415 0.67 -0.2 485,184 + Pf1N1B4_415 0.67 +0.5 485,184 + Pf1N1B4_415 0.67 -0.6 485,184 + Pf1N1B4_415 0.67 -1.3 485,184 + +0.3 485,185 - Pf1N1B4_415 0.67 -1.1 485,185 - Pf1N1B4_415 0.67 -0.3 485,185 - Pf1N1B4_415 0.67 +0.5 485,187 + +0.7 485,187 + Pf1N1B4_415 0.68 -1.0 485,187 + Pf1N1B4_415 0.68 +0.7 485,187 + Pf1N1B4_415 0.68 -0.9 485,187 + Pf1N1B4_415 0.68 +0.1 485,188 - Pf1N1B4_415 0.68 -0.3 485,188 - Pf1N1B4_415 0.68 +0.7 485,188 - Pf1N1B4_415 0.68 -1.6 485,188 - Pf1N1B4_415 0.68 +0.7 485,188 - Pf1N1B4_415 0.68 -0.0 485,188 - Pf1N1B4_415 0.68 -0.4 485,190 + Pf1N1B4_415 0.68 -2.7 485,190 + Pf1N1B4_415 0.68 +1.7 485,190 + Pf1N1B4_415 0.68 -0.2 485,190 + Pf1N1B4_415 0.68 -0.4 485,191 - +1.7 485,209 + Pf1N1B4_415 0.70 -0.1 485,258 + Pf1N1B4_415 0.76 +0.7 485,259 - Pf1N1B4_415 0.76 +0.7 485,259 - Pf1N1B4_415 0.76 -0.3 485,280 + Pf1N1B4_415 0.78 +0.0 485,280 + Pf1N1B4_415 0.78 -0.0 485,280 + -1.3 485,319 + Pf1N1B4_415 0.83 -2.2 485,430 + +0.7 485,431 - -0.4 485,460 - -0.2 485,460 - +1.7 485,469 + -2.1 485,470 - +0.5 485,630 - Pf1N1B4_416 0.18 -0.9 485,630 - Pf1N1B4_416 0.18 -1.6 485,630 - Pf1N1B4_416 0.18 -2.0 485,630 - Pf1N1B4_416 0.18 +0.6 485,680 + Pf1N1B4_416 0.27 -0.8 485,681 - Pf1N1B4_416 0.27 -1.2 485,724 - Pf1N1B4_416 0.35 -0.4 485,724 - Pf1N1B4_416 0.35 -0.5 485,878 + Pf1N1B4_416 0.62 -0.5 485,879 - Pf1N1B4_416 0.62 -0.3 485,879 - Pf1N1B4_416 0.62 +0.7 485,879 - Pf1N1B4_416 0.62 +0.0 485,879 - Pf1N1B4_416 0.62 +0.7 485,879 - +0.7 485,953 + Pf1N1B4_416 0.76 +1.7 485,953 + Pf1N1B4_416 0.76 -0.9 485,954 - Pf1N1B4_416 0.76 -0.2 485,954 - Pf1N1B4_416 0.76 +0.7 485,954 - Pf1N1B4_416 0.76 +0.9 485,956 + +0.7 485,956 + Pf1N1B4_416 0.76 -0.4 485,957 - Pf1N1B4_416 0.76 -3.0 485,957 - Pf1N1B4_416 0.76 -0.9 485,957 - Pf1N1B4_416 0.76 +0.8 485,957 - Pf1N1B4_416 0.76 -3.3 486,107 + -0.3 486,108 - +0.7 486,141 - +0.7 486,196 + Pf1N1B4_417 0.15 -2.9 486,216 + Pf1N1B4_417 0.18 +0.7 486,216 + Pf1N1B4_417 0.18 +0.7 486,216 + Pf1N1B4_417 0.18 -1.1 486,217 - Pf1N1B4_417 0.18 +0.7 486,226 - Pf1N1B4_417 0.20 -0.6 486,236 + Pf1N1B4_417 0.21 -2.3 486,365 + Pf1N1B4_417 0.43 -3.3 486,365 + Pf1N1B4_417 0.43 -2.5 486,365 + -0.6 486,365 + Pf1N1B4_417 0.43 -0.4 486,374 + Pf1N1B4_417 0.44 +0.0 486,455 + Pf1N1B4_417 0.58 -0.9 486,455 + Pf1N1B4_417 0.58 -0.9 486,456 - Pf1N1B4_417 0.58 +0.7
Or see this region's nucleotide sequence