Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4095 and Pf1N1B4_4096 are separated by 201 nucleotides Pf1N1B4_4096 and Pf1N1B4_4097 are separated by 17 nucleotides
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Pf1N1B4_4096 - Nucleoside-diphosphate-sugar epimerases, at 4,544,924 to 4,546,090
_4096
Pf1N1B4_4097 - Inosose isomerase (EC 5.3.99.-), at 4,546,108 to 4,547,190
_4097
Position (kb)
4544
4545
4546
4547 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4543.928 kb on - strand, within Pf1N1B4_4095 at 4544.388 kb on + strand, within Pf1N1B4_4095 at 4544.639 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.834 kb on - strand at 4544.946 kb on + strand at 4544.947 kb on - strand at 4545.113 kb on + strand, within Pf1N1B4_4096 at 4545.205 kb on - strand at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.431 kb on + strand, within Pf1N1B4_4096 at 4545.905 kb on + strand, within Pf1N1B4_4096 at 4546.270 kb on + strand at 4546.270 kb on + strand at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand, within Pf1N1B4_4097 at 4546.271 kb on - strand at 4546.306 kb on + strand, within Pf1N1B4_4097 at 4546.307 kb on - strand at 4546.564 kb on + strand, within Pf1N1B4_4097 at 4546.565 kb on - strand, within Pf1N1B4_4097 at 4546.565 kb on - strand, within Pf1N1B4_4097 at 4546.565 kb on - strand, within Pf1N1B4_4097 at 4546.682 kb on - strand at 4546.811 kb on - strand, within Pf1N1B4_4097 at 4546.901 kb on - strand, within Pf1N1B4_4097
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 4,543,928 - Pf1N1B4_4095 0.21 +0.6 4,544,388 + Pf1N1B4_4095 0.67 -0.6 4,544,639 + -3.0 4,544,751 + +1.6 4,544,751 + -1.0 4,544,751 + +0.8 4,544,752 - -0.4 4,544,752 - +0.6 4,544,752 - -0.8 4,544,834 - +0.6 4,544,946 + -0.1 4,544,947 - -1.7 4,545,113 + Pf1N1B4_4096 0.16 -2.7 4,545,205 - -3.7 4,545,347 + Pf1N1B4_4096 0.36 -0.3 4,545,347 + Pf1N1B4_4096 0.36 +0.6 4,545,347 + Pf1N1B4_4096 0.36 +0.6 4,545,348 - Pf1N1B4_4096 0.36 +1.3 4,545,348 - Pf1N1B4_4096 0.36 +0.6 4,545,382 + Pf1N1B4_4096 0.39 -2.0 4,545,382 + Pf1N1B4_4096 0.39 -0.0 4,545,431 + Pf1N1B4_4096 0.43 -0.1 4,545,905 + Pf1N1B4_4096 0.84 +0.2 4,546,270 + -2.9 4,546,270 + -0.7 4,546,271 - Pf1N1B4_4097 0.15 -0.5 4,546,271 - Pf1N1B4_4097 0.15 +0.6 4,546,271 - Pf1N1B4_4097 0.15 +0.0 4,546,271 - Pf1N1B4_4097 0.15 +0.5 4,546,271 - Pf1N1B4_4097 0.15 -0.0 4,546,271 - Pf1N1B4_4097 0.15 +0.0 4,546,271 - +0.6 4,546,306 + Pf1N1B4_4097 0.18 -0.7 4,546,307 - -2.6 4,546,564 + Pf1N1B4_4097 0.42 -0.7 4,546,565 - Pf1N1B4_4097 0.42 -2.4 4,546,565 - Pf1N1B4_4097 0.42 -0.2 4,546,565 - Pf1N1B4_4097 0.42 -0.5 4,546,682 - +0.6 4,546,811 - Pf1N1B4_4097 0.65 -1.4 4,546,901 - Pf1N1B4_4097 0.73 +0.6
Or see this region's nucleotide sequence