Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3807 and Pf1N1B4_3808 are separated by 263 nucleotides Pf1N1B4_3808 and Pf1N1B4_3809 are separated by 111 nucleotides Pf1N1B4_3809 and Pf1N1B4_3810 are separated by 124 nucleotides
Pf1N1B4_3807 - Rhs family protein, at 4,214,344 to 4,219,221
_3807
Pf1N1B4_3808 - hydrolase, haloacid dehalogenase-like family, at 4,219,485 to 4,220,180
_3808
Pf1N1B4_3809 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,220,292 to 4,221,038
_3809
Pf1N1B4_3810 - Transcriptional regulator, LysR family, at 4,221,163 to 4,222,089
_3810
Position (kb)
4219
4220
4221 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4218.708 kb on - strand, within Pf1N1B4_3807 at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.716 kb on + strand at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand at 4218.859 kb on - strand at 4218.930 kb on + strand at 4218.930 kb on + strand at 4218.930 kb on + strand at 4218.931 kb on - strand at 4219.402 kb on - strand at 4219.418 kb on + strand at 4219.528 kb on + strand at 4219.565 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.570 kb on - strand, within Pf1N1B4_3808 at 4219.671 kb on + strand, within Pf1N1B4_3808 at 4219.671 kb on + strand, within Pf1N1B4_3808 at 4219.700 kb on + strand, within Pf1N1B4_3808 at 4219.700 kb on + strand, within Pf1N1B4_3808 at 4219.817 kb on + strand, within Pf1N1B4_3808 at 4220.123 kb on + strand at 4220.124 kb on - strand at 4220.185 kb on + strand at 4220.185 kb on + strand at 4220.186 kb on - strand at 4220.186 kb on - strand at 4220.186 kb on - strand at 4220.249 kb on + strand at 4220.250 kb on - strand at 4220.339 kb on - strand at 4220.339 kb on - strand at 4220.362 kb on + strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.569 kb on + strand at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4221.009 kb on + strand at 4221.009 kb on + strand at 4221.009 kb on + strand at 4221.010 kb on - strand at 4221.010 kb on - strand at 4221.010 kb on - strand at 4221.101 kb on + strand at 4221.101 kb on + strand at 4221.101 kb on + strand at 4221.112 kb on + strand at 4221.126 kb on + strand at 4221.126 kb on + strand at 4221.127 kb on - strand at 4221.129 kb on - strand at 4221.129 kb on - strand at 4221.137 kb on + strand at 4221.150 kb on + strand at 4221.150 kb on + strand at 4221.150 kb on + strand at 4221.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 4,218,708 - Pf1N1B4_3807 0.89 -1.1 4,218,716 + Pf1N1B4_3807 0.90 -2.7 4,218,716 + Pf1N1B4_3807 0.90 -2.3 4,218,716 + -1.7 4,218,716 + Pf1N1B4_3807 0.90 -0.8 4,218,717 - Pf1N1B4_3807 0.90 -1.4 4,218,717 - Pf1N1B4_3807 0.90 -0.9 4,218,717 - Pf1N1B4_3807 0.90 -0.8 4,218,717 - Pf1N1B4_3807 0.90 -0.5 4,218,717 - +0.7 4,218,859 - +0.3 4,218,930 + +0.7 4,218,930 + -0.6 4,218,930 + -2.8 4,218,931 - +0.7 4,219,402 - -0.1 4,219,418 + -0.2 4,219,528 + -3.0 4,219,565 + Pf1N1B4_3808 0.11 +3.7 4,219,569 + Pf1N1B4_3808 0.12 -0.3 4,219,569 + Pf1N1B4_3808 0.12 -0.9 4,219,569 + Pf1N1B4_3808 0.12 +0.7 4,219,569 + Pf1N1B4_3808 0.12 -0.3 4,219,569 + Pf1N1B4_3808 0.12 -0.1 4,219,569 + Pf1N1B4_3808 0.12 +0.7 4,219,570 - Pf1N1B4_3808 0.12 +1.1 4,219,671 + Pf1N1B4_3808 0.27 +0.7 4,219,671 + Pf1N1B4_3808 0.27 -0.0 4,219,700 + Pf1N1B4_3808 0.31 -1.9 4,219,700 + Pf1N1B4_3808 0.31 +1.1 4,219,817 + Pf1N1B4_3808 0.48 +0.4 4,220,123 + -0.9 4,220,124 - +0.7 4,220,185 + -1.9 4,220,185 + +0.9 4,220,186 - -1.1 4,220,186 - -0.0 4,220,186 - +0.7 4,220,249 + -0.9 4,220,250 - -0.3 4,220,339 - -2.8 4,220,339 - -0.9 4,220,362 + +0.7 4,220,363 - +0.7 4,220,363 - -1.0 4,220,363 - -0.2 4,220,363 - -2.5 4,220,363 - -1.7 4,220,363 - +0.7 4,220,569 + -2.7 4,220,923 + Pf1N1B4_3809 0.84 -2.3 4,220,923 + Pf1N1B4_3809 0.84 +0.2 4,220,923 + Pf1N1B4_3809 0.84 +0.7 4,220,923 + Pf1N1B4_3809 0.84 -3.9 4,220,924 - Pf1N1B4_3809 0.85 +0.7 4,220,924 - Pf1N1B4_3809 0.85 +0.5 4,220,924 - Pf1N1B4_3809 0.85 -1.2 4,221,009 + +1.1 4,221,009 + -0.2 4,221,009 + +0.7 4,221,010 - -1.0 4,221,010 - -2.9 4,221,010 - -1.7 4,221,101 + +2.5 4,221,101 + +0.7 4,221,101 + +0.7 4,221,112 + -0.9 4,221,126 + -1.2 4,221,126 + -1.1 4,221,127 - -0.5 4,221,129 - -0.9 4,221,129 - -1.1 4,221,137 + -0.6 4,221,150 + -0.5 4,221,150 + +0.1 4,221,150 + +0.2 4,221,152 + -0.4
Or see this region's nucleotide sequence