Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotides Pf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotides Pf1N1B4_3415 and Pf1N1B4_3416 are separated by 215 nucleotides
Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652
_3413
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657
_3415
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Position (kb)
3805
3806 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.439 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand at 3804.817 kb on + strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.944 kb on + strand at 3804.945 kb on - strand at 3804.946 kb on + strand at 3804.947 kb on - strand at 3804.990 kb on + strand at 3804.991 kb on - strand at 3804.991 kb on - strand at 3804.995 kb on - strand at 3805.060 kb on - strand at 3805.060 kb on - strand at 3805.248 kb on - strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.528 kb on + strand, within Pf1N1B4_3415 at 3805.657 kb on + strand at 3805.657 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.704 kb on - strand at 3805.737 kb on - strand at 3805.737 kb on - strand at 3805.750 kb on + strand at 3805.761 kb on + strand at 3805.818 kb on + strand at 3805.819 kb on - strand at 3805.819 kb on - strand at 3805.884 kb on - strand at 3805.955 kb on + strand at 3805.956 kb on - strand at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.335 kb on + strand, within Pf1N1B4_3416 at 3806.336 kb on - strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.642 kb on - strand, within Pf1N1B4_3416
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 3,804,308 + Pf1N1B4_3413 0.61 +1.1 3,804,308 + Pf1N1B4_3413 0.61 +0.7 3,804,439 + Pf1N1B4_3413 0.76 -1.6 3,804,465 + Pf1N1B4_3413 0.79 +0.7 3,804,465 + -0.6 3,804,817 + Pf1N1B4_3414 0.63 +0.7 3,804,818 - Pf1N1B4_3414 0.64 +0.7 3,804,818 - Pf1N1B4_3414 0.64 -1.3 3,804,818 - Pf1N1B4_3414 0.64 -0.0 3,804,944 + -0.3 3,804,945 - +0.7 3,804,946 + -0.4 3,804,947 - +0.7 3,804,990 + +1.7 3,804,991 - -2.9 3,804,991 - +0.7 3,804,995 - -0.9 3,805,060 - -1.0 3,805,060 - -1.3 3,805,248 - Pf1N1B4_3415 0.33 +0.2 3,805,515 + Pf1N1B4_3415 0.77 +1.4 3,805,515 + Pf1N1B4_3415 0.77 +0.3 3,805,515 + Pf1N1B4_3415 0.77 -4.0 3,805,516 - Pf1N1B4_3415 0.77 +0.0 3,805,516 - Pf1N1B4_3415 0.77 -2.8 3,805,516 - Pf1N1B4_3415 0.77 +1.9 3,805,528 + Pf1N1B4_3415 0.79 +0.7 3,805,657 + +0.4 3,805,657 + +0.7 3,805,661 + +0.4 3,805,661 + +0.4 3,805,661 + +1.4 3,805,704 - +1.1 3,805,737 - -0.3 3,805,737 - +0.7 3,805,750 + -1.9 3,805,761 + +1.1 3,805,818 + +0.7 3,805,819 - +0.7 3,805,819 - -0.9 3,805,884 - +0.7 3,805,955 + -3.1 3,805,956 - -0.1 3,806,127 - Pf1N1B4_3416 0.24 -2.1 3,806,127 - Pf1N1B4_3416 0.24 +0.4 3,806,284 + Pf1N1B4_3416 0.39 +0.7 3,806,284 + Pf1N1B4_3416 0.39 +0.3 3,806,284 + Pf1N1B4_3416 0.39 +0.7 3,806,285 - Pf1N1B4_3416 0.39 +0.7 3,806,285 - Pf1N1B4_3416 0.39 -0.3 3,806,285 - Pf1N1B4_3416 0.39 +0.7 3,806,335 + Pf1N1B4_3416 0.44 -4.2 3,806,336 - Pf1N1B4_3416 0.44 -0.3 3,806,641 + Pf1N1B4_3416 0.73 +0.3 3,806,641 + Pf1N1B4_3416 0.73 +0.8 3,806,642 - Pf1N1B4_3416 0.73 -1.3
Or see this region's nucleotide sequence