Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3415

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotidesPf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotidesPf1N1B4_3415 and Pf1N1B4_3416 are separated by 215 nucleotides Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652 _3413 Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907 _3414 Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657 _3415 Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931 _3416 Position (kb) 3805 3806Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3804.308 kb on + strand, within Pf1N1B4_3413at 3804.308 kb on + strand, within Pf1N1B4_3413at 3804.439 kb on + strand, within Pf1N1B4_3413at 3804.465 kb on + strand, within Pf1N1B4_3413at 3804.465 kb on + strandat 3804.817 kb on + strand, within Pf1N1B4_3414at 3804.818 kb on - strand, within Pf1N1B4_3414at 3804.818 kb on - strand, within Pf1N1B4_3414at 3804.818 kb on - strand, within Pf1N1B4_3414at 3804.944 kb on + strandat 3804.945 kb on - strandat 3804.946 kb on + strandat 3804.947 kb on - strandat 3804.990 kb on + strandat 3804.991 kb on - strandat 3804.991 kb on - strandat 3804.995 kb on - strandat 3805.060 kb on - strandat 3805.060 kb on - strandat 3805.248 kb on - strand, within Pf1N1B4_3415at 3805.515 kb on + strand, within Pf1N1B4_3415at 3805.515 kb on + strand, within Pf1N1B4_3415at 3805.515 kb on + strand, within Pf1N1B4_3415at 3805.516 kb on - strand, within Pf1N1B4_3415at 3805.516 kb on - strand, within Pf1N1B4_3415at 3805.516 kb on - strand, within Pf1N1B4_3415at 3805.528 kb on + strand, within Pf1N1B4_3415at 3805.657 kb on + strandat 3805.657 kb on + strandat 3805.661 kb on + strandat 3805.661 kb on + strandat 3805.661 kb on + strandat 3805.704 kb on - strandat 3805.737 kb on - strandat 3805.737 kb on - strandat 3805.750 kb on + strandat 3805.761 kb on + strandat 3805.818 kb on + strandat 3805.819 kb on - strandat 3805.819 kb on - strandat 3805.884 kb on - strandat 3805.955 kb on + strandat 3805.956 kb on - strandat 3806.127 kb on - strand, within Pf1N1B4_3416at 3806.127 kb on - strand, within Pf1N1B4_3416at 3806.284 kb on + strand, within Pf1N1B4_3416at 3806.284 kb on + strand, within Pf1N1B4_3416at 3806.284 kb on + strand, within Pf1N1B4_3416at 3806.285 kb on - strand, within Pf1N1B4_3416at 3806.285 kb on - strand, within Pf1N1B4_3416at 3806.285 kb on - strand, within Pf1N1B4_3416at 3806.335 kb on + strand, within Pf1N1B4_3416at 3806.336 kb on - strand, within Pf1N1B4_3416at 3806.641 kb on + strand, within Pf1N1B4_3416at 3806.641 kb on + strand, within Pf1N1B4_3416at 3806.642 kb on - strand, within Pf1N1B4_3416

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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3,804,308 + Pf1N1B4_3413 0.61 +1.1
3,804,308 + Pf1N1B4_3413 0.61 +0.7
3,804,439 + Pf1N1B4_3413 0.76 -1.6
3,804,465 + Pf1N1B4_3413 0.79 +0.7
3,804,465 + -0.6
3,804,817 + Pf1N1B4_3414 0.63 +0.7
3,804,818 - Pf1N1B4_3414 0.64 +0.7
3,804,818 - Pf1N1B4_3414 0.64 -1.3
3,804,818 - Pf1N1B4_3414 0.64 -0.0
3,804,944 + -0.3
3,804,945 - +0.7
3,804,946 + -0.4
3,804,947 - +0.7
3,804,990 + +1.7
3,804,991 - -2.9
3,804,991 - +0.7
3,804,995 - -0.9
3,805,060 - -1.0
3,805,060 - -1.3
3,805,248 - Pf1N1B4_3415 0.33 +0.2
3,805,515 + Pf1N1B4_3415 0.77 +1.4
3,805,515 + Pf1N1B4_3415 0.77 +0.3
3,805,515 + Pf1N1B4_3415 0.77 -4.0
3,805,516 - Pf1N1B4_3415 0.77 +0.0
3,805,516 - Pf1N1B4_3415 0.77 -2.8
3,805,516 - Pf1N1B4_3415 0.77 +1.9
3,805,528 + Pf1N1B4_3415 0.79 +0.7
3,805,657 + +0.4
3,805,657 + +0.7
3,805,661 + +0.4
3,805,661 + +0.4
3,805,661 + +1.4
3,805,704 - +1.1
3,805,737 - -0.3
3,805,737 - +0.7
3,805,750 + -1.9
3,805,761 + +1.1
3,805,818 + +0.7
3,805,819 - +0.7
3,805,819 - -0.9
3,805,884 - +0.7
3,805,955 + -3.1
3,805,956 - -0.1
3,806,127 - Pf1N1B4_3416 0.24 -2.1
3,806,127 - Pf1N1B4_3416 0.24 +0.4
3,806,284 + Pf1N1B4_3416 0.39 +0.7
3,806,284 + Pf1N1B4_3416 0.39 +0.3
3,806,284 + Pf1N1B4_3416 0.39 +0.7
3,806,285 - Pf1N1B4_3416 0.39 +0.7
3,806,285 - Pf1N1B4_3416 0.39 -0.3
3,806,285 - Pf1N1B4_3416 0.39 +0.7
3,806,335 + Pf1N1B4_3416 0.44 -4.2
3,806,336 - Pf1N1B4_3416 0.44 -0.3
3,806,641 + Pf1N1B4_3416 0.73 +0.3
3,806,641 + Pf1N1B4_3416 0.73 +0.8
3,806,642 - Pf1N1B4_3416 0.73 -1.3

Or see this region's nucleotide sequence