Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_320

Experiment: N2E2wt with N1B4m denitrifying repA

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf1N1B4_318 and Pf1N1B4_319 are separated by 15 nucleotidesPf1N1B4_319 and Pf1N1B4_320 are separated by 47 nucleotidesPf1N1B4_320 and Pf1N1B4_321 are separated by 32 nucleotides Pf1N1B4_318 - NOHBY730; no homolog in Saccharomyces cerevisiae, at 374,762 to 375,613 _318 Pf1N1B4_319 - Transcriptional regulator, GntR family, at 375,629 to 376,306 _319 Pf1N1B4_320 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 376,354 to 377,628 _320 Pf1N1B4_321 - Probable short-chain dehydrogenase, at 377,661 to 378,455 _321 Position (kb) 376 377 378Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 375.543 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.609 kb on + strandat 375.610 kb on - strandat 375.610 kb on - strandat 375.687 kb on + strandat 375.687 kb on + strandat 375.688 kb on - strandat 375.688 kb on - strandat 375.688 kb on - strandat 375.743 kb on + strand, within Pf1N1B4_319at 375.743 kb on + strand, within Pf1N1B4_319at 375.743 kb on + strand, within Pf1N1B4_319at 375.743 kb on + strand, within Pf1N1B4_319at 375.743 kb on + strand, within Pf1N1B4_319at 375.743 kb on + strand, within Pf1N1B4_319at 375.744 kb on - strand, within Pf1N1B4_319at 375.744 kb on - strand, within Pf1N1B4_319at 375.744 kb on - strand, within Pf1N1B4_319at 375.744 kb on - strand, within Pf1N1B4_319at 375.744 kb on - strand, within Pf1N1B4_319at 375.744 kb on - strandat 375.744 kb on - strand, within Pf1N1B4_319at 376.084 kb on + strand, within Pf1N1B4_319at 376.102 kb on - strand, within Pf1N1B4_319at 376.102 kb on - strand, within Pf1N1B4_319at 376.102 kb on - strand, within Pf1N1B4_319at 376.168 kb on - strand, within Pf1N1B4_319at 376.168 kb on - strand, within Pf1N1B4_319at 376.168 kb on - strand, within Pf1N1B4_319at 376.283 kb on - strandat 376.325 kb on + strandat 376.326 kb on - strandat 376.332 kb on + strandat 376.333 kb on - strandat 376.333 kb on - strandat 376.346 kb on - strandat 376.346 kb on - strandat 376.346 kb on - strandat 376.346 kb on - strandat 376.356 kb on - strandat 376.356 kb on - strandat 376.356 kb on - strandat 376.356 kb on - strandat 376.368 kb on - strandat 376.368 kb on - strandat 376.424 kb on + strandat 376.491 kb on + strand, within Pf1N1B4_320at 376.500 kb on + strandat 376.501 kb on - strand, within Pf1N1B4_320at 376.797 kb on - strand, within Pf1N1B4_320at 376.797 kb on - strand, within Pf1N1B4_320at 376.797 kb on - strandat 376.965 kb on - strandat 377.049 kb on + strand, within Pf1N1B4_320at 377.049 kb on + strand, within Pf1N1B4_320at 377.050 kb on - strand, within Pf1N1B4_320at 377.050 kb on - strand, within Pf1N1B4_320at 377.379 kb on + strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.380 kb on - strand, within Pf1N1B4_320at 377.638 kb on - strandat 377.638 kb on - strandat 377.638 kb on - strandat 377.669 kb on - strandat 377.691 kb on - strandat 377.691 kb on - strandat 377.691 kb on - strandat 377.726 kb on + strandat 377.726 kb on + strandat 377.727 kb on - strandat 377.885 kb on + strand, within Pf1N1B4_321at 377.886 kb on - strand, within Pf1N1B4_321at 377.971 kb on + strandat 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strand, within Pf1N1B4_321at 377.972 kb on - strandat 377.989 kb on + strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 377.990 kb on - strand, within Pf1N1B4_321at 378.039 kb on - strand, within Pf1N1B4_321at 378.186 kb on - strand, within Pf1N1B4_321at 378.539 kb on + strandat 378.540 kb on - strandat 378.540 kb on - strandat 378.553 kb on + strandat 378.554 kb on - strandat 378.554 kb on - strandat 378.558 kb on + strandat 378.565 kb on - strandat 378.613 kb on - strandat 378.613 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
remove
375,543 + -0.9
375,609 + +1.4
375,609 + +0.0
375,609 + -0.3
375,609 + +0.8
375,609 + +0.8
375,609 + +1.3
375,609 + -1.9
375,609 + -0.5
375,609 + +0.7
375,610 - -0.3
375,610 - +0.1
375,687 + +0.8
375,687 + +0.7
375,688 - -2.7
375,688 - -0.5
375,688 - -3.5
375,743 + Pf1N1B4_319 0.17 -1.1
375,743 + Pf1N1B4_319 0.17 +0.7
375,743 + Pf1N1B4_319 0.17 -0.6
375,743 + Pf1N1B4_319 0.17 +0.7
375,743 + Pf1N1B4_319 0.17 +0.7
375,743 + Pf1N1B4_319 0.17 -0.1
375,744 - Pf1N1B4_319 0.17 -1.3
375,744 - Pf1N1B4_319 0.17 -1.6
375,744 - Pf1N1B4_319 0.17 +0.7
375,744 - Pf1N1B4_319 0.17 -1.0
375,744 - Pf1N1B4_319 0.17 -2.7
375,744 - +0.7
375,744 - Pf1N1B4_319 0.17 -2.5
376,084 + Pf1N1B4_319 0.67 -0.3
376,102 - Pf1N1B4_319 0.70 -0.6
376,102 - Pf1N1B4_319 0.70 -1.3
376,102 - Pf1N1B4_319 0.70 -1.7
376,168 - Pf1N1B4_319 0.79 +0.2
376,168 - Pf1N1B4_319 0.79 -0.9
376,168 - Pf1N1B4_319 0.79 +0.7
376,283 - -1.7
376,325 + -0.8
376,326 - -1.3
376,332 + -0.1
376,333 - -0.1
376,333 - +0.7
376,346 - +0.7
376,346 - -0.3
376,346 - +0.7
376,346 - -2.8
376,356 - +0.7
376,356 - -0.9
376,356 - +0.7
376,356 - -1.7
376,368 - +0.7
376,368 - -0.9
376,424 + +1.4
376,491 + Pf1N1B4_320 0.11 -0.7
376,500 + +0.7
376,501 - Pf1N1B4_320 0.12 +0.9
376,797 - Pf1N1B4_320 0.35 +1.8
376,797 - Pf1N1B4_320 0.35 -3.8
376,797 - +0.7
376,965 - +0.7
377,049 + Pf1N1B4_320 0.55 +0.1
377,049 + Pf1N1B4_320 0.55 -0.1
377,050 - Pf1N1B4_320 0.55 +0.9
377,050 - Pf1N1B4_320 0.55 +3.1
377,379 + Pf1N1B4_320 0.80 -1.9
377,380 - Pf1N1B4_320 0.80 +0.2
377,380 - Pf1N1B4_320 0.80 +0.7
377,380 - Pf1N1B4_320 0.80 +0.7
377,380 - Pf1N1B4_320 0.80 -0.9
377,638 - -0.3
377,638 - -0.6
377,638 - +1.4
377,669 - -0.2
377,691 - -3.0
377,691 - -0.5
377,691 - -0.9
377,726 + +0.9
377,726 + -1.6
377,727 - -0.2
377,885 + Pf1N1B4_321 0.28 -1.3
377,886 - Pf1N1B4_321 0.28 -0.7
377,971 + -3.4
377,972 - Pf1N1B4_321 0.39 -1.5
377,972 - Pf1N1B4_321 0.39 -0.3
377,972 - Pf1N1B4_321 0.39 -0.6
377,972 - Pf1N1B4_321 0.39 -0.2
377,972 - Pf1N1B4_321 0.39 -1.9
377,972 - Pf1N1B4_321 0.39 -3.9
377,972 - Pf1N1B4_321 0.39 -0.5
377,972 - Pf1N1B4_321 0.39 +0.0
377,972 - Pf1N1B4_321 0.39 -0.3
377,972 - Pf1N1B4_321 0.39 -1.5
377,972 - Pf1N1B4_321 0.39 +0.7
377,972 - Pf1N1B4_321 0.39 -0.5
377,972 - Pf1N1B4_321 0.39 +0.0
377,972 - +1.1
377,989 + Pf1N1B4_321 0.41 -0.5
377,990 - Pf1N1B4_321 0.41 -4.3
377,990 - Pf1N1B4_321 0.41 -2.1
377,990 - Pf1N1B4_321 0.41 -3.1
378,039 - Pf1N1B4_321 0.48 -2.1
378,186 - Pf1N1B4_321 0.66 +0.4
378,539 + -1.7
378,540 - -0.5
378,540 - -0.4
378,553 + -2.0
378,554 - -1.7
378,554 - -2.1
378,558 + -3.9
378,565 - +0.5
378,613 - -0.1
378,613 - +1.0

Or see this region's nucleotide sequence