Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_310 and Pf1N1B4_311 are separated by 141 nucleotides Pf1N1B4_311 and Pf1N1B4_312 are separated by 48 nucleotides
Pf1N1B4_310 - UPF0028 protein YchK, at 363,216 to 364,256
_310
Pf1N1B4_311 - sensor histidine kinase, at 364,398 to 365,096
_311
Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796
_312
Position (kb)
364
365
366 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 363.411 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand at 364.101 kb on + strand, within Pf1N1B4_310 at 364.102 kb on - strand, within Pf1N1B4_310 at 364.173 kb on + strand at 364.173 kb on + strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.234 kb on - strand at 364.399 kb on + strand at 364.399 kb on + strand at 364.399 kb on + strand at 364.451 kb on + strand at 364.506 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.619 kb on - strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.824 kb on + strand, within Pf1N1B4_311 at 364.824 kb on + strand, within Pf1N1B4_311 at 365.105 kb on - strand at 365.131 kb on + strand at 365.326 kb on + strand at 365.326 kb on + strand at 365.326 kb on + strand at 365.327 kb on - strand at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.512 kb on + strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.513 kb on - strand, within Pf1N1B4_312 at 365.664 kb on + strand, within Pf1N1B4_312 at 365.665 kb on - strand, within Pf1N1B4_312 at 365.665 kb on - strand, within Pf1N1B4_312 at 365.665 kb on - strand, within Pf1N1B4_312 at 365.679 kb on - strand, within Pf1N1B4_312 at 365.679 kb on - strand, within Pf1N1B4_312 at 365.680 kb on + strand, within Pf1N1B4_312 at 365.680 kb on + strand, within Pf1N1B4_312 at 365.680 kb on + strand, within Pf1N1B4_312 at 365.680 kb on + strand, within Pf1N1B4_312 at 365.681 kb on - strand, within Pf1N1B4_312 at 365.808 kb on + strand, within Pf1N1B4_312 at 365.809 kb on - strand, within Pf1N1B4_312
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 363,411 + Pf1N1B4_310 0.19 +0.1 363,509 + Pf1N1B4_310 0.28 +0.7 363,509 + Pf1N1B4_310 0.28 -0.4 363,510 - Pf1N1B4_310 0.28 +0.7 363,510 - Pf1N1B4_310 0.28 -0.3 363,512 + Pf1N1B4_310 0.28 +0.7 363,512 + Pf1N1B4_310 0.28 -1.3 363,512 + Pf1N1B4_310 0.28 +1.2 363,771 + Pf1N1B4_310 0.53 -0.9 363,771 + -0.3 364,101 + Pf1N1B4_310 0.85 -1.5 364,102 - Pf1N1B4_310 0.85 -1.7 364,173 + -2.6 364,173 + -1.1 364,174 - -2.7 364,174 - -2.9 364,174 - -0.2 364,174 - -0.3 364,174 - -2.4 364,234 - -1.1 364,399 + +0.7 364,399 + +1.2 364,399 + -1.5 364,451 + +0.8 364,506 + Pf1N1B4_311 0.15 -0.2 364,516 + Pf1N1B4_311 0.17 -0.8 364,516 + Pf1N1B4_311 0.17 -1.1 364,516 + Pf1N1B4_311 0.17 +0.7 364,516 + Pf1N1B4_311 0.17 +0.9 364,618 + Pf1N1B4_311 0.31 -0.3 364,618 + Pf1N1B4_311 0.31 -1.7 364,618 + Pf1N1B4_311 0.31 -0.8 364,618 + Pf1N1B4_311 0.31 +0.7 364,619 - Pf1N1B4_311 0.32 +0.7 364,795 + Pf1N1B4_311 0.57 -1.9 364,795 + Pf1N1B4_311 0.57 +0.7 364,795 + Pf1N1B4_311 0.57 +0.7 364,795 + Pf1N1B4_311 0.57 -0.3 364,796 - Pf1N1B4_311 0.57 -1.9 364,796 - Pf1N1B4_311 0.57 -2.0 364,796 - Pf1N1B4_311 0.57 -1.4 364,824 + Pf1N1B4_311 0.61 -0.3 364,824 + Pf1N1B4_311 0.61 +0.7 365,105 - +0.9 365,131 + +0.7 365,326 + -2.1 365,326 + +0.2 365,326 + +0.7 365,327 - -2.1 365,512 + Pf1N1B4_312 0.14 +1.5 365,512 + Pf1N1B4_312 0.14 -0.2 365,512 + Pf1N1B4_312 0.14 +0.7 365,512 + Pf1N1B4_312 0.14 +0.4 365,512 + -2.3 365,512 + Pf1N1B4_312 0.14 -2.3 365,512 + Pf1N1B4_312 0.14 -0.2 365,512 + Pf1N1B4_312 0.14 -2.1 365,512 + Pf1N1B4_312 0.14 -0.9 365,512 + Pf1N1B4_312 0.14 -1.6 365,512 + Pf1N1B4_312 0.14 -1.0 365,512 + Pf1N1B4_312 0.14 +0.7 365,512 + Pf1N1B4_312 0.14 +0.7 365,512 + Pf1N1B4_312 0.14 -3.2 365,512 + Pf1N1B4_312 0.14 -0.9 365,512 + Pf1N1B4_312 0.14 +2.1 365,513 - Pf1N1B4_312 0.14 -1.7 365,513 - Pf1N1B4_312 0.14 +0.7 365,513 - Pf1N1B4_312 0.14 -1.3 365,513 - Pf1N1B4_312 0.14 +0.1 365,513 - Pf1N1B4_312 0.14 +0.7 365,513 - Pf1N1B4_312 0.14 +0.7 365,513 - Pf1N1B4_312 0.14 +0.7 365,664 + Pf1N1B4_312 0.20 +1.7 365,665 - Pf1N1B4_312 0.20 +0.7 365,665 - Pf1N1B4_312 0.20 -2.8 365,665 - Pf1N1B4_312 0.20 -1.8 365,679 - Pf1N1B4_312 0.20 -3.1 365,679 - Pf1N1B4_312 0.20 +0.7 365,680 + Pf1N1B4_312 0.20 -0.0 365,680 + Pf1N1B4_312 0.20 -0.4 365,680 + Pf1N1B4_312 0.20 -2.8 365,680 + Pf1N1B4_312 0.20 +0.7 365,681 - Pf1N1B4_312 0.20 -0.8 365,808 + Pf1N1B4_312 0.25 +0.7 365,809 - Pf1N1B4_312 0.25 -0.3
Or see this region's nucleotide sequence