Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2974

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2972 and Pf1N1B4_2973 overlap by 1 nucleotidesPf1N1B4_2973 and Pf1N1B4_2974 overlap by 4 nucleotidesPf1N1B4_2974 and Pf1N1B4_2975 are separated by 154 nucleotidesPf1N1B4_2975 and Pf1N1B4_2976 are separated by 43 nucleotides Pf1N1B4_2972 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,333,196 to 3,334,257 _2972 Pf1N1B4_2973 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,334,257 to 3,334,721 _2973 Pf1N1B4_2974 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 3,334,718 to 3,335,434 _2974 Pf1N1B4_2975 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 3,335,589 to 3,336,104 _2975 Pf1N1B4_2976 - DipZ protein, at 3,336,148 to 3,337,935 _2976 Position (kb) 3334 3335 3336Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3334.195 kb on - strandat 3334.258 kb on + strandat 3334.258 kb on + strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.259 kb on - strandat 3334.262 kb on + strandat 3334.262 kb on + strandat 3334.262 kb on + strandat 3334.262 kb on + strandat 3334.262 kb on + strandat 3334.262 kb on + strandat 3334.263 kb on - strandat 3334.263 kb on - strandat 3334.268 kb on + strandat 3334.468 kb on + strand, within Pf1N1B4_2973at 3334.469 kb on - strand, within Pf1N1B4_2973at 3334.469 kb on - strand, within Pf1N1B4_2973at 3334.750 kb on - strandat 3334.849 kb on - strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3335.130 kb on + strand, within Pf1N1B4_2974at 3335.378 kb on - strandat 3335.494 kb on + strandat 3335.494 kb on + strandat 3335.495 kb on - strandat 3335.508 kb on + strandat 3335.544 kb on + strandat 3335.544 kb on + strandat 3335.545 kb on - strandat 3335.545 kb on - strandat 3335.656 kb on + strand, within Pf1N1B4_2975at 3335.657 kb on - strand, within Pf1N1B4_2975at 3335.657 kb on - strand, within Pf1N1B4_2975at 3335.806 kb on - strandat 3335.806 kb on - strand, within Pf1N1B4_2975at 3335.834 kb on + strand, within Pf1N1B4_2975at 3335.835 kb on - strand, within Pf1N1B4_2975at 3336.060 kb on + strandat 3336.102 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.148 kb on - strandat 3336.148 kb on - strandat 3336.169 kb on + strandat 3336.170 kb on - strandat 3336.356 kb on - strand, within Pf1N1B4_2976

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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3,334,195 - +0.7
3,334,258 + -2.3
3,334,258 + -1.3
3,334,259 - +2.0
3,334,259 - -3.7
3,334,259 - +0.7
3,334,259 - +0.9
3,334,259 - +0.7
3,334,259 - -0.6
3,334,259 - -1.3
3,334,262 + -2.6
3,334,262 + -2.0
3,334,262 + -2.3
3,334,262 + -1.7
3,334,262 + +0.7
3,334,262 + -1.4
3,334,263 - -0.9
3,334,263 - -0.9
3,334,268 + -2.3
3,334,468 + Pf1N1B4_2973 0.45 -2.9
3,334,469 - Pf1N1B4_2973 0.46 -0.9
3,334,469 - Pf1N1B4_2973 0.46 +0.7
3,334,750 - -2.9
3,334,849 - Pf1N1B4_2974 0.18 +0.7
3,334,929 + Pf1N1B4_2974 0.29 -0.3
3,334,929 + Pf1N1B4_2974 0.29 +2.5
3,334,929 + Pf1N1B4_2974 0.29 -0.6
3,334,930 - Pf1N1B4_2974 0.30 +1.9
3,334,930 - Pf1N1B4_2974 0.30 +1.0
3,334,930 - Pf1N1B4_2974 0.30 -0.3
3,334,930 - Pf1N1B4_2974 0.30 -2.1
3,334,930 - Pf1N1B4_2974 0.30 +0.7
3,335,130 + Pf1N1B4_2974 0.57 -0.0
3,335,378 - -2.3
3,335,494 + -2.5
3,335,494 + -0.5
3,335,495 - +0.5
3,335,508 + -1.9
3,335,544 + -2.0
3,335,544 + -2.0
3,335,545 - -0.5
3,335,545 - +0.7
3,335,656 + Pf1N1B4_2975 0.13 -2.1
3,335,657 - Pf1N1B4_2975 0.13 +0.7
3,335,657 - Pf1N1B4_2975 0.13 -3.0
3,335,806 - -0.3
3,335,806 - Pf1N1B4_2975 0.42 +0.7
3,335,834 + Pf1N1B4_2975 0.47 +0.5
3,335,835 - Pf1N1B4_2975 0.48 -1.3
3,336,060 + +0.3
3,336,102 + -0.1
3,336,147 + -0.2
3,336,147 + +0.7
3,336,147 + +0.7
3,336,147 + +0.7
3,336,147 + -4.2
3,336,147 + +0.6
3,336,148 - -0.9
3,336,148 - -1.6
3,336,169 + -4.1
3,336,170 - -0.7
3,336,356 - Pf1N1B4_2976 0.12 +0.1

Or see this region's nucleotide sequence