Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2846 and Pf1N1B4_2847 are separated by 106 nucleotides Pf1N1B4_2847 and Pf1N1B4_2848 are separated by 409 nucleotides
Pf1N1B4_2846 - putative exported protein, at 3,190,015 to 3,192,960
_2846
Pf1N1B4_2847 - Methyltransferase (EC 2.1.1.-), at 3,193,067 to 3,194,083
_2847
Pf1N1B4_2848 - Mobile element protein, at 3,194,493 to 3,195,521
_2848
Position (kb)
3193
3194
3195 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3192.073 kb on + strand, within Pf1N1B4_2846 at 3192.168 kb on + strand, within Pf1N1B4_2846 at 3192.168 kb on + strand, within Pf1N1B4_2846 at 3192.168 kb on + strand, within Pf1N1B4_2846 at 3192.168 kb on + strand, within Pf1N1B4_2846 at 3192.169 kb on - strand, within Pf1N1B4_2846 at 3192.169 kb on - strand, within Pf1N1B4_2846 at 3192.169 kb on - strand, within Pf1N1B4_2846 at 3192.172 kb on + strand, within Pf1N1B4_2846 at 3192.173 kb on - strand, within Pf1N1B4_2846 at 3192.173 kb on - strand, within Pf1N1B4_2846 at 3192.173 kb on - strand, within Pf1N1B4_2846 at 3192.269 kb on + strand, within Pf1N1B4_2846 at 3192.312 kb on + strand, within Pf1N1B4_2846 at 3192.312 kb on + strand, within Pf1N1B4_2846 at 3192.313 kb on - strand, within Pf1N1B4_2846 at 3192.486 kb on + strand, within Pf1N1B4_2846 at 3192.625 kb on - strand at 3192.836 kb on + strand at 3192.836 kb on + strand at 3192.837 kb on - strand at 3192.880 kb on + strand at 3192.903 kb on + strand at 3192.904 kb on - strand at 3192.999 kb on - strand at 3193.036 kb on - strand at 3193.037 kb on + strand at 3193.037 kb on + strand at 3193.037 kb on + strand at 3193.037 kb on + strand at 3193.044 kb on + strand at 3193.044 kb on + strand at 3193.044 kb on + strand at 3193.045 kb on - strand at 3193.045 kb on - strand at 3193.045 kb on - strand at 3193.045 kb on - strand at 3193.245 kb on - strand, within Pf1N1B4_2847 at 3193.356 kb on - strand, within Pf1N1B4_2847 at 3193.365 kb on + strand, within Pf1N1B4_2847 at 3193.389 kb on + strand, within Pf1N1B4_2847 at 3193.389 kb on + strand, within Pf1N1B4_2847 at 3193.389 kb on + strand, within Pf1N1B4_2847 at 3193.389 kb on + strand, within Pf1N1B4_2847 at 3193.390 kb on - strand, within Pf1N1B4_2847 at 3193.390 kb on - strand, within Pf1N1B4_2847 at 3193.458 kb on + strand, within Pf1N1B4_2847 at 3193.459 kb on - strand, within Pf1N1B4_2847 at 3193.553 kb on - strand, within Pf1N1B4_2847 at 3193.605 kb on + strand, within Pf1N1B4_2847 at 3193.606 kb on - strand, within Pf1N1B4_2847 at 3193.810 kb on + strand, within Pf1N1B4_2847 at 3194.123 kb on + strand at 3194.123 kb on + strand at 3194.124 kb on - strand at 3194.150 kb on - strand at 3194.150 kb on - strand at 3194.150 kb on - strand at 3194.150 kb on - strand at 3194.155 kb on + strand at 3194.155 kb on + strand at 3194.242 kb on - strand at 3194.242 kb on - strand at 3194.242 kb on - strand at 3194.242 kb on - strand at 3194.242 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand at 3194.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 3,192,073 + Pf1N1B4_2846 0.70 -0.6 3,192,168 + Pf1N1B4_2846 0.73 -1.3 3,192,168 + Pf1N1B4_2846 0.73 -0.5 3,192,168 + Pf1N1B4_2846 0.73 -1.4 3,192,168 + Pf1N1B4_2846 0.73 -0.4 3,192,169 - Pf1N1B4_2846 0.73 +0.7 3,192,169 - Pf1N1B4_2846 0.73 -0.9 3,192,169 - Pf1N1B4_2846 0.73 -4.1 3,192,172 + Pf1N1B4_2846 0.73 -1.4 3,192,173 - Pf1N1B4_2846 0.73 -0.8 3,192,173 - Pf1N1B4_2846 0.73 +0.7 3,192,173 - Pf1N1B4_2846 0.73 -2.0 3,192,269 + Pf1N1B4_2846 0.77 -2.5 3,192,312 + Pf1N1B4_2846 0.78 +0.7 3,192,312 + Pf1N1B4_2846 0.78 -1.8 3,192,313 - Pf1N1B4_2846 0.78 -2.7 3,192,486 + Pf1N1B4_2846 0.84 -3.2 3,192,625 - +0.7 3,192,836 + -1.3 3,192,836 + -3.3 3,192,837 - -1.7 3,192,880 + -1.1 3,192,903 + +0.7 3,192,904 - -0.9 3,192,999 - +0.5 3,193,036 - -0.1 3,193,037 + +0.7 3,193,037 + +0.6 3,193,037 + -2.6 3,193,037 + +0.7 3,193,044 + -3.9 3,193,044 + -2.9 3,193,044 + -0.3 3,193,045 - -2.1 3,193,045 - +0.7 3,193,045 - -0.5 3,193,045 - +0.3 3,193,245 - Pf1N1B4_2847 0.18 -0.3 3,193,356 - Pf1N1B4_2847 0.28 -0.6 3,193,365 + Pf1N1B4_2847 0.29 +0.7 3,193,389 + Pf1N1B4_2847 0.32 +0.7 3,193,389 + Pf1N1B4_2847 0.32 -1.6 3,193,389 + Pf1N1B4_2847 0.32 -0.6 3,193,389 + Pf1N1B4_2847 0.32 -0.4 3,193,390 - Pf1N1B4_2847 0.32 -0.5 3,193,390 - Pf1N1B4_2847 0.32 +1.1 3,193,458 + Pf1N1B4_2847 0.38 +1.6 3,193,459 - Pf1N1B4_2847 0.39 +0.5 3,193,553 - Pf1N1B4_2847 0.48 -0.3 3,193,605 + Pf1N1B4_2847 0.53 +2.2 3,193,606 - Pf1N1B4_2847 0.53 -3.0 3,193,810 + Pf1N1B4_2847 0.73 -0.3 3,194,123 + +0.7 3,194,123 + +0.2 3,194,124 - -3.7 3,194,150 - +0.5 3,194,150 - -2.6 3,194,150 - -0.4 3,194,150 - -0.7 3,194,155 + -0.9 3,194,155 + +0.7 3,194,242 - +0.7 3,194,242 - +2.0 3,194,242 - -2.5 3,194,242 - -2.1 3,194,242 - -0.8 3,194,248 - -1.2 3,194,248 - -1.1 3,194,248 - +0.7 3,194,248 - +0.7 3,194,248 - -0.9 3,194,248 - -0.6 3,194,248 - -0.8 3,194,248 - +0.7
Or see this region's nucleotide sequence