Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_283 and Pf1N1B4_284 are separated by 113 nucleotides Pf1N1B4_284 and Pf1N1B4_285 are separated by 96 nucleotides
Pf1N1B4_283 - Cys regulon transcriptional activator CysB, at 339,949 to 340,923
_283
Pf1N1B4_284 - Universal stress protein family, at 341,037 to 341,540
_284
Pf1N1B4_285 - 5'-nucleotidase (EC 3.1.3.5), at 341,637 to 342,542
_285
Position (kb) 341
342 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 340.944 kb on - strand at 340.944 kb on - strand at 340.944 kb on - strand at 340.969 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.979 kb on - strand at 341.003 kb on - strand at 341.039 kb on - strand at 341.039 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.167 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.383 kb on + strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.500 kb on + strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.583 kb on + strand at 341.584 kb on - strand at 341.584 kb on - strand at 341.639 kb on - strand at 341.654 kb on + strand at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.734 kb on - strand, within Pf1N1B4_285 at 342.019 kb on + strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.260 kb on - strand, within Pf1N1B4_285 at 342.260 kb on - strand, within Pf1N1B4_285 at 342.269 kb on + strand at 342.269 kb on + strand, within Pf1N1B4_285 at 342.269 kb on + strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.321 kb on + strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.428 kb on - strand, within Pf1N1B4_285 at 342.428 kb on - strand, within Pf1N1B4_285 at 342.461 kb on - strand at 342.461 kb on - strand at 342.461 kb on - strand at 342.461 kb on - strand at 342.528 kb on + strand at 342.529 kb on - strand at 342.529 kb on - strand at 342.529 kb on - strand at 342.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 340,944 - -0.3 340,944 - -0.3 340,944 - -0.3 340,969 - -3.0 340,971 - -0.7 340,971 - -2.3 340,971 - +1.2 340,971 - -0.4 340,971 - -1.7 340,971 - +0.7 340,971 - +1.1 340,977 - -1.3 340,977 - -0.3 340,977 - +0.7 340,979 - +0.7 341,003 - -1.9 341,039 - -0.2 341,039 - -0.2 341,081 - -4.7 341,081 - -3.7 341,081 - -0.7 341,081 - -2.6 341,167 - Pf1N1B4_284 0.26 -2.1 341,337 - Pf1N1B4_284 0.60 -1.3 341,337 - Pf1N1B4_284 0.60 -2.9 341,383 + Pf1N1B4_284 0.69 +0.7 341,384 - Pf1N1B4_284 0.69 -4.8 341,384 - Pf1N1B4_284 0.69 -3.7 341,384 - Pf1N1B4_284 0.69 -2.1 341,384 - Pf1N1B4_284 0.69 -3.5 341,384 - Pf1N1B4_284 0.69 -4.1 341,384 - Pf1N1B4_284 0.69 -2.8 341,384 - Pf1N1B4_284 0.69 -3.5 341,440 + Pf1N1B4_284 0.80 -1.8 341,440 + Pf1N1B4_284 0.80 +0.7 341,440 + +0.7 341,440 + Pf1N1B4_284 0.80 -0.3 341,440 + Pf1N1B4_284 0.80 -3.2 341,440 + Pf1N1B4_284 0.80 -1.3 341,440 + Pf1N1B4_284 0.80 -2.9 341,440 + Pf1N1B4_284 0.80 -2.7 341,440 + Pf1N1B4_284 0.80 -2.0 341,440 + Pf1N1B4_284 0.80 -3.3 341,440 + Pf1N1B4_284 0.80 -2.9 341,440 + Pf1N1B4_284 0.80 +0.1 341,440 + Pf1N1B4_284 0.80 -0.5 341,440 + Pf1N1B4_284 0.80 -3.5 341,440 + Pf1N1B4_284 0.80 -1.4 341,440 + +0.7 341,440 + Pf1N1B4_284 0.80 -2.7 341,440 + Pf1N1B4_284 0.80 -2.5 341,440 + Pf1N1B4_284 0.80 -4.3 341,441 - Pf1N1B4_284 0.80 -1.7 341,441 - Pf1N1B4_284 0.80 -3.0 341,441 - Pf1N1B4_284 0.80 -1.9 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 -1.9 341,441 - Pf1N1B4_284 0.80 -0.9 341,441 - Pf1N1B4_284 0.80 -2.9 341,441 - Pf1N1B4_284 0.80 -1.7 341,441 - Pf1N1B4_284 0.80 -5.0 341,441 - Pf1N1B4_284 0.80 -1.1 341,441 - Pf1N1B4_284 0.80 -4.5 341,441 - Pf1N1B4_284 0.80 -4.1 341,441 - Pf1N1B4_284 0.80 -0.9 341,441 - Pf1N1B4_284 0.80 -1.7 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 -1.8 341,441 - Pf1N1B4_284 0.80 +0.7 341,441 - Pf1N1B4_284 0.80 -2.2 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 +0.7 341,441 - Pf1N1B4_284 0.80 -3.2 341,441 - Pf1N1B4_284 0.80 -1.5 341,441 - Pf1N1B4_284 0.80 +0.7 341,441 - -3.0 341,441 - Pf1N1B4_284 0.80 -3.5 341,441 - Pf1N1B4_284 0.80 -3.4 341,441 - Pf1N1B4_284 0.80 -2.8 341,441 - Pf1N1B4_284 0.80 -0.8 341,441 - Pf1N1B4_284 0.80 -3.1 341,441 - Pf1N1B4_284 0.80 -0.6 341,441 - Pf1N1B4_284 0.80 -4.0 341,441 - Pf1N1B4_284 0.80 -1.0 341,441 - Pf1N1B4_284 0.80 -2.8 341,441 - -0.3 341,441 - Pf1N1B4_284 0.80 -2.9 341,441 - Pf1N1B4_284 0.80 -1.9 341,441 - Pf1N1B4_284 0.80 -4.7 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -3.5 341,441 - Pf1N1B4_284 0.80 -1.7 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 -3.2 341,441 - Pf1N1B4_284 0.80 -0.6 341,441 - Pf1N1B4_284 0.80 -3.0 341,441 - Pf1N1B4_284 0.80 -1.5 341,441 - Pf1N1B4_284 0.80 -3.5 341,441 - Pf1N1B4_284 0.80 -0.2 341,500 + -3.0 341,501 - -3.8 341,501 - -1.5 341,501 - -1.5 341,501 - -1.4 341,501 - -2.7 341,501 - -5.4 341,501 - -2.1 341,501 - -0.8 341,501 - -1.6 341,521 + +0.7 341,521 + -2.7 341,521 + -2.9 341,521 + -1.7 341,521 + -1.8 341,521 + -3.4 341,521 + -2.9 341,521 + -1.7 341,522 - -1.3 341,522 - -3.7 341,522 - -1.4 341,522 - -1.3 341,522 - -1.6 341,522 - -3.7 341,522 - -2.0 341,522 - -2.7 341,522 - -4.1 341,522 - -5.0 341,522 - -2.8 341,522 - -1.6 341,522 - -1.0 341,522 - -2.4 341,522 - -3.1 341,522 - -3.4 341,522 - -1.3 341,522 - -2.6 341,522 - -3.0 341,522 - -2.0 341,522 - +0.4 341,522 - -1.9 341,583 + -4.5 341,584 - +0.3 341,584 - -0.3 341,639 - -0.6 341,654 + -0.1 341,733 + Pf1N1B4_285 0.11 +0.7 341,733 + Pf1N1B4_285 0.11 -3.8 341,733 + Pf1N1B4_285 0.11 -3.1 341,734 - Pf1N1B4_285 0.11 +1.2 342,019 + Pf1N1B4_285 0.42 -0.3 342,020 - Pf1N1B4_285 0.42 -2.9 342,020 - Pf1N1B4_285 0.42 -1.8 342,020 - Pf1N1B4_285 0.42 -1.1 342,117 - Pf1N1B4_285 0.53 -1.7 342,117 - Pf1N1B4_285 0.53 -1.5 342,117 - Pf1N1B4_285 0.53 +0.7 342,117 - Pf1N1B4_285 0.53 +0.1 342,117 - Pf1N1B4_285 0.53 -0.5 342,206 - Pf1N1B4_285 0.63 +0.7 342,206 - Pf1N1B4_285 0.63 -1.1 342,206 - Pf1N1B4_285 0.63 -0.4 342,260 - Pf1N1B4_285 0.69 -1.2 342,260 - Pf1N1B4_285 0.69 -0.3 342,269 + -3.6 342,269 + Pf1N1B4_285 0.70 +0.2 342,269 + Pf1N1B4_285 0.70 -1.3 342,270 - Pf1N1B4_285 0.70 -2.9 342,270 - Pf1N1B4_285 0.70 -1.2 342,270 - Pf1N1B4_285 0.70 -0.9 342,270 - Pf1N1B4_285 0.70 -0.5 342,270 - Pf1N1B4_285 0.70 -1.9 342,270 - Pf1N1B4_285 0.70 -0.1 342,270 - Pf1N1B4_285 0.70 +0.7 342,321 + Pf1N1B4_285 0.75 +0.8 342,322 - Pf1N1B4_285 0.76 -0.9 342,322 - Pf1N1B4_285 0.76 -1.1 342,322 - Pf1N1B4_285 0.76 -1.5 342,428 - Pf1N1B4_285 0.87 -1.0 342,428 - Pf1N1B4_285 0.87 +0.7 342,461 - -3.2 342,461 - -0.9 342,461 - -0.6 342,461 - -1.3 342,528 + +0.7 342,529 - +0.2 342,529 - -2.1 342,529 - +0.7 342,529 - -0.3
Or see this region's nucleotide sequence