Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2673 and Pf1N1B4_2674 are separated by 103 nucleotides Pf1N1B4_2674 and Pf1N1B4_2675 overlap by 15 nucleotides
Pf1N1B4_2673 - Aldehyde dehydrogenase (EC 1.2.1.3), at 3,010,438 to 3,011,958
_2673
Pf1N1B4_2674 - hypothetical protein, at 3,012,062 to 3,012,424
_2674
Pf1N1B4_2675 - Nitrogen regulation protein NR(I), at 3,012,410 to 3,014,323
_2675
Position (kb)
3012
3013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3011.274 kb on + strand, within Pf1N1B4_2673 at 3011.412 kb on + strand, within Pf1N1B4_2673 at 3011.514 kb on - strand, within Pf1N1B4_2673 at 3011.820 kb on + strand at 3011.820 kb on + strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.845 kb on - strand at 3011.845 kb on - strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.899 kb on - strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.951 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.989 kb on - strand at 3012.016 kb on + strand at 3012.023 kb on - strand at 3012.058 kb on + strand at 3012.233 kb on + strand, within Pf1N1B4_2674 at 3012.234 kb on - strand, within Pf1N1B4_2674 at 3012.372 kb on - strand, within Pf1N1B4_2674 at 3012.401 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.536 kb on + strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.806 kb on - strand, within Pf1N1B4_2675 at 3012.883 kb on + strand, within Pf1N1B4_2675 at 3012.884 kb on - strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.040 kb on + strand, within Pf1N1B4_2675 at 3013.041 kb on - strand, within Pf1N1B4_2675 at 3013.041 kb on - strand at 3013.041 kb on - strand, within Pf1N1B4_2675 at 3013.052 kb on + strand, within Pf1N1B4_2675 at 3013.052 kb on + strand, within Pf1N1B4_2675 at 3013.053 kb on - strand, within Pf1N1B4_2675 at 3013.189 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.348 kb on + strand, within Pf1N1B4_2675 at 3013.349 kb on - strand, within Pf1N1B4_2675
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 3,011,274 + Pf1N1B4_2673 0.55 -1.3 3,011,412 + Pf1N1B4_2673 0.64 +0.7 3,011,514 - Pf1N1B4_2673 0.71 +0.7 3,011,820 + +0.0 3,011,820 + +1.1 3,011,821 - -0.3 3,011,821 - +1.3 3,011,821 - +0.3 3,011,821 - -1.5 3,011,821 - +1.9 3,011,821 - +0.7 3,011,845 - +1.1 3,011,845 - -0.9 3,011,894 + +0.7 3,011,894 + +0.2 3,011,894 + -1.1 3,011,894 + +0.7 3,011,894 + -0.9 3,011,894 + -0.1 3,011,894 + -0.8 3,011,894 + +0.7 3,011,894 + -0.7 3,011,894 + -0.4 3,011,894 + -0.1 3,011,895 - -0.9 3,011,895 - -2.6 3,011,895 - +0.7 3,011,895 - +1.5 3,011,895 - -1.5 3,011,895 - -3.1 3,011,895 - +1.1 3,011,895 - -0.6 3,011,899 - -0.0 3,011,903 + -1.0 3,011,903 + -0.3 3,011,903 + +0.7 3,011,903 + -1.6 3,011,903 + +0.7 3,011,903 + -1.2 3,011,903 + -1.7 3,011,904 - +0.7 3,011,904 - -1.5 3,011,904 - +0.7 3,011,951 + +0.1 3,011,986 + +0.7 3,011,986 + +1.0 3,011,986 + -0.3 3,011,986 + -0.5 3,011,986 + +0.4 3,011,986 + -1.3 3,011,986 + -0.7 3,011,987 - +0.7 3,011,987 - -1.9 3,011,987 - -1.5 3,011,987 - +0.0 3,011,988 + -0.9 3,011,988 + +0.7 3,011,988 + +0.7 3,011,988 + +1.4 3,011,989 - +0.7 3,012,016 + -0.6 3,012,023 - +0.7 3,012,058 + -1.1 3,012,233 + Pf1N1B4_2674 0.47 -1.3 3,012,234 - Pf1N1B4_2674 0.47 -0.3 3,012,372 - Pf1N1B4_2674 0.85 +0.7 3,012,401 + +0.7 3,012,404 + -0.3 3,012,404 + +0.6 3,012,404 + +0.7 3,012,405 - +1.0 3,012,405 - -1.3 3,012,405 - +0.7 3,012,536 + -2.7 3,012,537 - +0.7 3,012,537 - +0.7 3,012,537 - -2.1 3,012,806 - Pf1N1B4_2675 0.21 +0.7 3,012,883 + Pf1N1B4_2675 0.25 +0.7 3,012,884 - Pf1N1B4_2675 0.25 -1.6 3,013,040 + Pf1N1B4_2675 0.33 +0.7 3,013,040 + Pf1N1B4_2675 0.33 -2.0 3,013,040 + Pf1N1B4_2675 0.33 -0.3 3,013,041 - Pf1N1B4_2675 0.33 -0.2 3,013,041 - +0.7 3,013,041 - Pf1N1B4_2675 0.33 +0.7 3,013,052 + Pf1N1B4_2675 0.34 +0.7 3,013,052 + Pf1N1B4_2675 0.34 +0.3 3,013,053 - Pf1N1B4_2675 0.34 +1.5 3,013,189 + Pf1N1B4_2675 0.41 -0.0 3,013,348 + Pf1N1B4_2675 0.49 -3.1 3,013,348 + Pf1N1B4_2675 0.49 +0.9 3,013,348 + Pf1N1B4_2675 0.49 -1.3 3,013,349 - Pf1N1B4_2675 0.49 +2.7
Or see this region's nucleotide sequence