Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2559

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2557 and Pf1N1B4_2558 are separated by 33 nucleotidesPf1N1B4_2558 and Pf1N1B4_2559 are separated by 135 nucleotidesPf1N1B4_2559 and Pf1N1B4_2560 are separated by 13 nucleotidesPf1N1B4_2560 and Pf1N1B4_2561 are separated by 38 nucleotidesPf1N1B4_2561 and Pf1N1B4_2562 are separated by 74 nucleotides Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290 _2557 Pf1N1B4_2558 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 2,900,324 to 2,900,755 _2558 Pf1N1B4_2559 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,900,891 to 2,901,925 _2559 Pf1N1B4_2560 - hypothetical protein, at 2,901,939 to 2,902,055 _2560 Pf1N1B4_2561 - probable iron binding protein from the HesB_IscA_SufA family, at 2,902,094 to 2,902,444 _2561 Pf1N1B4_2562 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 2,902,519 to 2,903,610 _2562 Position (kb) 2900 2901 2902Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2899.941 kb on + strand, within Pf1N1B4_2557at 2899.941 kb on + strand, within Pf1N1B4_2557at 2899.941 kb on + strand, within Pf1N1B4_2557at 2900.000 kb on + strand, within Pf1N1B4_2557at 2900.076 kb on + strand, within Pf1N1B4_2557at 2900.076 kb on + strandat 2900.180 kb on + strand, within Pf1N1B4_2557at 2900.224 kb on + strandat 2900.225 kb on - strandat 2900.243 kb on + strandat 2900.244 kb on - strandat 2900.244 kb on - strandat 2900.325 kb on + strandat 2900.325 kb on + strandat 2900.326 kb on - strandat 2900.760 kb on - strandat 2900.760 kb on - strandat 2900.760 kb on - strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.789 kb on + strandat 2900.790 kb on - strandat 2900.790 kb on - strandat 2900.790 kb on - strandat 2900.790 kb on - strandat 2900.790 kb on - strandat 2900.792 kb on + strandat 2900.792 kb on + strandat 2900.849 kb on + strandat 2900.849 kb on + strandat 2900.890 kb on + strandat 2900.890 kb on + strandat 2902.001 kb on + strand, within Pf1N1B4_2560at 2902.002 kb on - strandat 2902.002 kb on - strand, within Pf1N1B4_2560at 2902.003 kb on + strand, within Pf1N1B4_2560at 2902.003 kb on + strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.004 kb on - strand, within Pf1N1B4_2560at 2902.008 kb on + strand, within Pf1N1B4_2560at 2902.009 kb on - strand, within Pf1N1B4_2560at 2902.014 kb on + strand, within Pf1N1B4_2560at 2902.063 kb on + strandat 2902.459 kb on + strandat 2902.459 kb on + strandat 2902.459 kb on + strandat 2902.459 kb on + strandat 2902.460 kb on - strandat 2902.460 kb on - strandat 2902.460 kb on - strandat 2902.460 kb on - strandat 2902.460 kb on - strandat 2902.460 kb on - strandat 2902.462 kb on + strandat 2902.463 kb on - strandat 2902.463 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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2,899,941 + Pf1N1B4_2557 0.64 -0.3
2,899,941 + Pf1N1B4_2557 0.64 +0.7
2,899,941 + Pf1N1B4_2557 0.64 +0.7
2,900,000 + Pf1N1B4_2557 0.70 -2.2
2,900,076 + Pf1N1B4_2557 0.78 -0.5
2,900,076 + +0.6
2,900,180 + Pf1N1B4_2557 0.89 -3.9
2,900,224 + -2.9
2,900,225 - +0.7
2,900,243 + +0.7
2,900,244 - -1.0
2,900,244 - -1.2
2,900,325 + +0.2
2,900,325 + +0.7
2,900,326 - -1.3
2,900,760 - -2.3
2,900,760 - -1.5
2,900,760 - +0.7
2,900,789 + +1.7
2,900,789 + +0.7
2,900,789 + -2.5
2,900,789 + -2.6
2,900,789 + +0.2
2,900,789 + +0.7
2,900,789 + +0.7
2,900,789 + +0.7
2,900,789 + +0.7
2,900,789 + +0.7
2,900,789 + -1.6
2,900,789 + -0.9
2,900,789 + -2.3
2,900,789 + -1.9
2,900,789 + +0.1
2,900,789 + -0.7
2,900,790 - +0.7
2,900,790 - +0.7
2,900,790 - -2.9
2,900,790 - +0.3
2,900,790 - -0.9
2,900,792 + +1.3
2,900,792 + -2.7
2,900,849 + +0.7
2,900,849 + -1.3
2,900,890 + +0.7
2,900,890 + -0.9
2,902,001 + Pf1N1B4_2560 0.53 -1.7
2,902,002 - -1.0
2,902,002 - Pf1N1B4_2560 0.54 +0.7
2,902,003 + Pf1N1B4_2560 0.55 +0.7
2,902,003 + Pf1N1B4_2560 0.55 -1.5
2,902,004 - Pf1N1B4_2560 0.56 +0.7
2,902,004 - Pf1N1B4_2560 0.56 +0.2
2,902,004 - Pf1N1B4_2560 0.56 -0.8
2,902,004 - Pf1N1B4_2560 0.56 -0.6
2,902,004 - Pf1N1B4_2560 0.56 -0.4
2,902,004 - Pf1N1B4_2560 0.56 -1.7
2,902,004 - Pf1N1B4_2560 0.56 -1.2
2,902,004 - Pf1N1B4_2560 0.56 -1.6
2,902,004 - Pf1N1B4_2560 0.56 -0.7
2,902,004 - Pf1N1B4_2560 0.56 +0.5
2,902,004 - Pf1N1B4_2560 0.56 +0.5
2,902,004 - Pf1N1B4_2560 0.56 +0.5
2,902,008 + Pf1N1B4_2560 0.59 -2.5
2,902,009 - Pf1N1B4_2560 0.60 +0.7
2,902,014 + Pf1N1B4_2560 0.64 +0.7
2,902,063 + +0.7
2,902,459 + -1.6
2,902,459 + -3.2
2,902,459 + +0.3
2,902,459 + -0.6
2,902,460 - +0.9
2,902,460 - +1.8
2,902,460 - -0.3
2,902,460 - -1.9
2,902,460 - -3.1
2,902,460 - +0.7
2,902,462 + -2.3
2,902,463 - +0.7
2,902,463 - +0.7

Or see this region's nucleotide sequence