Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2159 and Pf1N1B4_2160 overlap by 1 nucleotides Pf1N1B4_2160 and Pf1N1B4_2161 are separated by 34 nucleotides
Pf1N1B4_2159 - Benzoate transport protein, at 2,473,309 to 2,474,559
_2159
Pf1N1B4_2160 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 2,474,559 to 2,475,413
_2160
Pf1N1B4_2161 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 2,475,448 to 2,476,713
_2161
Position (kb)
2474
2475
2476 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2473.590 kb on + strand, within Pf1N1B4_2159 at 2473.590 kb on + strand, within Pf1N1B4_2159 at 2473.590 kb on + strand, within Pf1N1B4_2159 at 2473.590 kb on + strand, within Pf1N1B4_2159 at 2473.593 kb on + strand, within Pf1N1B4_2159 at 2473.593 kb on + strand, within Pf1N1B4_2159 at 2473.593 kb on + strand, within Pf1N1B4_2159 at 2473.594 kb on - strand, within Pf1N1B4_2159 at 2473.594 kb on - strand, within Pf1N1B4_2159 at 2473.594 kb on - strand, within Pf1N1B4_2159 at 2473.613 kb on + strand, within Pf1N1B4_2159 at 2473.613 kb on + strand, within Pf1N1B4_2159 at 2473.613 kb on + strand, within Pf1N1B4_2159 at 2473.613 kb on + strand, within Pf1N1B4_2159 at 2473.613 kb on + strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.614 kb on - strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.621 kb on + strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.622 kb on - strand, within Pf1N1B4_2159 at 2473.726 kb on - strand, within Pf1N1B4_2159 at 2473.804 kb on + strand, within Pf1N1B4_2159 at 2473.805 kb on - strand, within Pf1N1B4_2159 at 2473.805 kb on - strand, within Pf1N1B4_2159 at 2473.892 kb on + strand at 2473.892 kb on + strand, within Pf1N1B4_2159 at 2473.893 kb on - strand, within Pf1N1B4_2159 at 2473.893 kb on - strand, within Pf1N1B4_2159 at 2473.893 kb on - strand, within Pf1N1B4_2159 at 2473.893 kb on - strand, within Pf1N1B4_2159 at 2473.893 kb on - strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.011 kb on + strand, within Pf1N1B4_2159 at 2474.012 kb on - strand, within Pf1N1B4_2159 at 2474.012 kb on - strand, within Pf1N1B4_2159 at 2474.030 kb on + strand, within Pf1N1B4_2159 at 2474.067 kb on + strand, within Pf1N1B4_2159 at 2474.096 kb on + strand, within Pf1N1B4_2159 at 2474.097 kb on - strand, within Pf1N1B4_2159 at 2474.224 kb on + strand, within Pf1N1B4_2159 at 2474.224 kb on + strand, within Pf1N1B4_2159 at 2474.224 kb on + strand, within Pf1N1B4_2159 at 2474.225 kb on - strand, within Pf1N1B4_2159 at 2474.225 kb on - strand, within Pf1N1B4_2159 at 2474.235 kb on - strand, within Pf1N1B4_2159 at 2474.292 kb on + strand, within Pf1N1B4_2159 at 2474.385 kb on + strand, within Pf1N1B4_2159 at 2474.385 kb on + strand, within Pf1N1B4_2159 at 2474.386 kb on - strand, within Pf1N1B4_2159 at 2474.386 kb on - strand, within Pf1N1B4_2159 at 2474.468 kb on - strand at 2474.508 kb on - strand at 2474.541 kb on + strand at 2474.766 kb on + strand, within Pf1N1B4_2160 at 2474.767 kb on - strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.012 kb on - strand, within Pf1N1B4_2160 at 2475.033 kb on + strand, within Pf1N1B4_2160 at 2475.033 kb on + strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.070 kb on - strand, within Pf1N1B4_2160 at 2475.163 kb on + strand, within Pf1N1B4_2160 at 2475.164 kb on - strand, within Pf1N1B4_2160 at 2475.333 kb on + strand at 2475.333 kb on + strand at 2475.333 kb on + strand at 2475.334 kb on - strand at 2475.334 kb on - strand at 2475.336 kb on + strand at 2475.336 kb on + strand at 2475.411 kb on + strand at 2475.411 kb on + strand at 2475.412 kb on - strand at 2475.412 kb on - strand at 2475.412 kb on - strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.611 kb on - strand, within Pf1N1B4_2161 at 2475.637 kb on + strand, within Pf1N1B4_2161 at 2475.637 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.641 kb on - strand, within Pf1N1B4_2161 at 2475.655 kb on + strand, within Pf1N1B4_2161 at 2475.656 kb on - strand, within Pf1N1B4_2161 at 2475.715 kb on + strand, within Pf1N1B4_2161 at 2475.865 kb on + strand, within Pf1N1B4_2161 at 2475.914 kb on - strand, within Pf1N1B4_2161 at 2475.931 kb on - strand, within Pf1N1B4_2161 at 2476.196 kb on - strand, within Pf1N1B4_2161 at 2476.309 kb on + strand, within Pf1N1B4_2161
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 2,473,590 + Pf1N1B4_2159 0.22 +0.8 2,473,590 + Pf1N1B4_2159 0.22 -0.8 2,473,590 + Pf1N1B4_2159 0.22 +0.8 2,473,590 + Pf1N1B4_2159 0.22 +0.8 2,473,593 + Pf1N1B4_2159 0.23 -4.1 2,473,593 + Pf1N1B4_2159 0.23 +0.8 2,473,593 + Pf1N1B4_2159 0.23 +0.5 2,473,594 - Pf1N1B4_2159 0.23 -0.4 2,473,594 - Pf1N1B4_2159 0.23 -0.3 2,473,594 - Pf1N1B4_2159 0.23 -3.0 2,473,613 + Pf1N1B4_2159 0.24 -1.4 2,473,613 + Pf1N1B4_2159 0.24 -0.8 2,473,613 + Pf1N1B4_2159 0.24 -0.8 2,473,613 + Pf1N1B4_2159 0.24 -1.0 2,473,613 + Pf1N1B4_2159 0.24 -2.2 2,473,614 - Pf1N1B4_2159 0.24 -0.2 2,473,614 - Pf1N1B4_2159 0.24 -1.5 2,473,614 - Pf1N1B4_2159 0.24 -2.2 2,473,614 - Pf1N1B4_2159 0.24 +1.1 2,473,614 - Pf1N1B4_2159 0.24 -0.5 2,473,614 - Pf1N1B4_2159 0.24 +0.8 2,473,621 + Pf1N1B4_2159 0.25 -2.9 2,473,621 + Pf1N1B4_2159 0.25 +0.8 2,473,621 + Pf1N1B4_2159 0.25 -2.7 2,473,621 + Pf1N1B4_2159 0.25 -0.2 2,473,621 + Pf1N1B4_2159 0.25 -1.8 2,473,621 + Pf1N1B4_2159 0.25 -1.8 2,473,621 + Pf1N1B4_2159 0.25 +1.8 2,473,621 + Pf1N1B4_2159 0.25 -0.8 2,473,621 + Pf1N1B4_2159 0.25 -1.3 2,473,621 + Pf1N1B4_2159 0.25 -2.2 2,473,621 + Pf1N1B4_2159 0.25 -3.1 2,473,621 + Pf1N1B4_2159 0.25 +0.8 2,473,621 + Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 -1.2 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 +1.3 2,473,622 - Pf1N1B4_2159 0.25 +0.4 2,473,622 - Pf1N1B4_2159 0.25 -2.2 2,473,622 - Pf1N1B4_2159 0.25 +1.1 2,473,622 - Pf1N1B4_2159 0.25 -0.1 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 -0.9 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 -0.2 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 +0.1 2,473,622 - Pf1N1B4_2159 0.25 +0.8 2,473,622 - Pf1N1B4_2159 0.25 -1.4 2,473,726 - Pf1N1B4_2159 0.33 -1.3 2,473,804 + Pf1N1B4_2159 0.40 -0.4 2,473,805 - Pf1N1B4_2159 0.40 +0.8 2,473,805 - Pf1N1B4_2159 0.40 -3.6 2,473,892 + +0.8 2,473,892 + Pf1N1B4_2159 0.47 +0.8 2,473,893 - Pf1N1B4_2159 0.47 -0.2 2,473,893 - Pf1N1B4_2159 0.47 +0.8 2,473,893 - Pf1N1B4_2159 0.47 -3.4 2,473,893 - Pf1N1B4_2159 0.47 +0.8 2,473,893 - Pf1N1B4_2159 0.47 -2.2 2,474,011 + Pf1N1B4_2159 0.56 -0.6 2,474,011 + Pf1N1B4_2159 0.56 +0.8 2,474,011 + Pf1N1B4_2159 0.56 -1.2 2,474,011 + Pf1N1B4_2159 0.56 -1.8 2,474,011 + Pf1N1B4_2159 0.56 +0.8 2,474,011 + Pf1N1B4_2159 0.56 -1.1 2,474,012 - Pf1N1B4_2159 0.56 -0.7 2,474,012 - Pf1N1B4_2159 0.56 +0.8 2,474,030 + Pf1N1B4_2159 0.58 -2.0 2,474,067 + Pf1N1B4_2159 0.61 +0.8 2,474,096 + Pf1N1B4_2159 0.63 +0.8 2,474,097 - Pf1N1B4_2159 0.63 -0.2 2,474,224 + Pf1N1B4_2159 0.73 +0.8 2,474,224 + Pf1N1B4_2159 0.73 +0.8 2,474,224 + Pf1N1B4_2159 0.73 -2.2 2,474,225 - Pf1N1B4_2159 0.73 -2.2 2,474,225 - Pf1N1B4_2159 0.73 +0.8 2,474,235 - Pf1N1B4_2159 0.74 -0.5 2,474,292 + Pf1N1B4_2159 0.79 -0.0 2,474,385 + Pf1N1B4_2159 0.86 +0.6 2,474,385 + Pf1N1B4_2159 0.86 -2.2 2,474,386 - Pf1N1B4_2159 0.86 +0.9 2,474,386 - Pf1N1B4_2159 0.86 -1.4 2,474,468 - -0.0 2,474,508 - +0.8 2,474,541 + -1.5 2,474,766 + Pf1N1B4_2160 0.24 -1.2 2,474,767 - Pf1N1B4_2160 0.24 -1.3 2,475,011 + Pf1N1B4_2160 0.53 -0.8 2,475,011 + Pf1N1B4_2160 0.53 +0.8 2,475,011 + Pf1N1B4_2160 0.53 -1.6 2,475,012 - Pf1N1B4_2160 0.53 -0.2 2,475,033 + Pf1N1B4_2160 0.55 -1.2 2,475,033 + Pf1N1B4_2160 0.55 +0.8 2,475,034 - Pf1N1B4_2160 0.56 -2.2 2,475,034 - Pf1N1B4_2160 0.56 -1.2 2,475,034 - Pf1N1B4_2160 0.56 +0.8 2,475,070 - Pf1N1B4_2160 0.60 +0.8 2,475,163 + Pf1N1B4_2160 0.71 -2.4 2,475,164 - Pf1N1B4_2160 0.71 -0.2 2,475,333 + -1.5 2,475,333 + +0.8 2,475,333 + +0.8 2,475,334 - +0.2 2,475,334 - -1.9 2,475,336 + -1.3 2,475,336 + -2.8 2,475,411 + -1.5 2,475,411 + +0.8 2,475,412 - -0.4 2,475,412 - -2.4 2,475,412 - +0.8 2,475,421 + -1.3 2,475,421 + -2.4 2,475,421 + -1.9 2,475,421 + +0.8 2,475,421 + -0.7 2,475,421 + +0.1 2,475,422 - -1.2 2,475,422 - -2.4 2,475,422 - +0.8 2,475,422 - +0.3 2,475,422 - +0.8 2,475,447 + -1.8 2,475,447 + -2.4 2,475,447 + -1.0 2,475,447 + -0.2 2,475,447 + +0.1 2,475,447 + +0.8 2,475,447 + +0.8 2,475,447 + +0.8 2,475,447 + -2.7 2,475,447 + -0.6 2,475,447 + -0.2 2,475,447 + +0.8 2,475,447 + -1.1 2,475,447 + +0.8 2,475,447 + -0.7 2,475,447 + +0.8 2,475,447 + -0.8 2,475,447 + -3.0 2,475,447 + -0.1 2,475,448 - -2.1 2,475,448 - -1.5 2,475,448 - +0.8 2,475,448 - -0.7 2,475,448 - +0.8 2,475,448 - -0.8 2,475,448 - -0.6 2,475,448 - +2.8 2,475,448 - -2.8 2,475,448 - +1.4 2,475,448 - -1.5 2,475,448 - +0.8 2,475,448 - +0.8 2,475,448 - +1.1 2,475,448 - -2.2 2,475,448 - -1.8 2,475,451 + -2.0 2,475,451 + -1.5 2,475,451 + -2.5 2,475,451 + -1.5 2,475,451 + +0.8 2,475,451 + +0.8 2,475,451 + -0.2 2,475,451 + +0.8 2,475,451 + -0.6 2,475,451 + +1.6 2,475,452 - +0.8 2,475,452 - +0.8 2,475,452 - -2.9 2,475,452 - -1.8 2,475,452 - -1.2 2,475,452 - -0.8 2,475,452 - +1.6 2,475,452 - +0.6 2,475,452 - +0.8 2,475,606 + Pf1N1B4_2161 0.12 -1.9 2,475,606 + Pf1N1B4_2161 0.12 +0.8 2,475,606 + Pf1N1B4_2161 0.12 -2.1 2,475,606 + Pf1N1B4_2161 0.12 -0.1 2,475,607 - Pf1N1B4_2161 0.13 -2.9 2,475,607 - Pf1N1B4_2161 0.13 -2.5 2,475,607 - Pf1N1B4_2161 0.13 -2.5 2,475,610 + Pf1N1B4_2161 0.13 -3.5 2,475,610 + Pf1N1B4_2161 0.13 +0.0 2,475,610 + Pf1N1B4_2161 0.13 -1.4 2,475,610 + Pf1N1B4_2161 0.13 -2.3 2,475,611 - Pf1N1B4_2161 0.13 +0.8 2,475,637 + Pf1N1B4_2161 0.15 -1.5 2,475,637 + Pf1N1B4_2161 0.15 +0.8 2,475,640 + Pf1N1B4_2161 0.15 -0.3 2,475,640 + Pf1N1B4_2161 0.15 -2.0 2,475,640 + Pf1N1B4_2161 0.15 -2.4 2,475,640 + Pf1N1B4_2161 0.15 +0.7 2,475,641 - Pf1N1B4_2161 0.15 +0.8 2,475,655 + Pf1N1B4_2161 0.16 -1.5 2,475,656 - Pf1N1B4_2161 0.16 -4.1 2,475,715 + Pf1N1B4_2161 0.21 +0.8 2,475,865 + Pf1N1B4_2161 0.33 -1.1 2,475,914 - Pf1N1B4_2161 0.37 -1.7 2,475,931 - Pf1N1B4_2161 0.38 +0.2 2,476,196 - Pf1N1B4_2161 0.59 -1.2 2,476,309 + Pf1N1B4_2161 0.68 -1.6
Or see this region's nucleotide sequence