Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2141 and Pf1N1B4_2142 are separated by 147 nucleotides Pf1N1B4_2142 and Pf1N1B4_2143 are separated by 116 nucleotides Pf1N1B4_2143 and Pf1N1B4_2144 are separated by 156 nucleotides
Pf1N1B4_2141 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 2,451,963 to 2,453,276
_2141
Pf1N1B4_2142 - FIG00954130: hypothetical protein, at 2,453,424 to 2,453,777
_2142
Pf1N1B4_2143 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 2,453,894 to 2,454,382
_2143
Pf1N1B4_2144 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 2,454,539 to 2,455,843
_2144
Position (kb)
2453
2454 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2452.480 kb on + strand at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.480 kb on + strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.481 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.535 kb on - strand, within Pf1N1B4_2141 at 2452.739 kb on - strand, within Pf1N1B4_2141 at 2453.221 kb on + strand at 2453.221 kb on + strand at 2453.222 kb on - strand at 2453.224 kb on + strand at 2453.224 kb on + strand at 2453.224 kb on + strand at 2453.224 kb on + strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.225 kb on - strand at 2453.276 kb on + strand at 2453.320 kb on + strand at 2453.320 kb on + strand at 2453.320 kb on + strand at 2453.321 kb on - strand at 2453.321 kb on - strand at 2453.321 kb on - strand at 2453.400 kb on - strand at 2453.400 kb on - strand at 2453.456 kb on - strand at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.136 kb on - strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.205 kb on - strand, within Pf1N1B4_2143 at 2454.383 kb on - strand at 2454.443 kb on - strand at 2454.443 kb on - strand at 2454.444 kb on + strand at 2454.445 kb on - strand at 2454.473 kb on + strand at 2454.502 kb on + strand at 2454.503 kb on - strand at 2454.503 kb on - strand at 2454.533 kb on - strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.588 kb on - strand at 2454.588 kb on - strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.689 kb on + strand, within Pf1N1B4_2144 at 2454.689 kb on + strand, within Pf1N1B4_2144
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 2,452,480 + +0.8 2,452,480 + Pf1N1B4_2141 0.39 +0.8 2,452,480 + Pf1N1B4_2141 0.39 +0.6 2,452,480 + Pf1N1B4_2141 0.39 -0.1 2,452,480 + Pf1N1B4_2141 0.39 +0.8 2,452,480 + Pf1N1B4_2141 0.39 -1.3 2,452,480 + Pf1N1B4_2141 0.39 -2.1 2,452,481 - Pf1N1B4_2141 0.39 +0.8 2,452,481 - Pf1N1B4_2141 0.39 +1.5 2,452,481 - Pf1N1B4_2141 0.39 +0.8 2,452,535 - Pf1N1B4_2141 0.44 -3.0 2,452,535 - Pf1N1B4_2141 0.44 +0.8 2,452,535 - Pf1N1B4_2141 0.44 +0.8 2,452,535 - Pf1N1B4_2141 0.44 -0.8 2,452,739 - Pf1N1B4_2141 0.59 +0.8 2,453,221 + +0.8 2,453,221 + +0.8 2,453,222 - +0.8 2,453,224 + +0.8 2,453,224 + +0.8 2,453,224 + -0.1 2,453,224 + +0.3 2,453,225 - +0.8 2,453,225 - +0.8 2,453,225 - +0.6 2,453,225 - +0.8 2,453,225 - +0.8 2,453,225 - +0.4 2,453,225 - -1.2 2,453,225 - -0.5 2,453,225 - -1.1 2,453,225 - -0.5 2,453,225 - -1.3 2,453,276 + -1.8 2,453,320 + -2.0 2,453,320 + -1.8 2,453,320 + -2.5 2,453,321 - +0.8 2,453,321 - +0.3 2,453,321 - -0.7 2,453,400 - -0.8 2,453,400 - +1.2 2,453,456 - +0.8 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 -1.5 2,453,628 + Pf1N1B4_2142 0.58 -1.6 2,453,628 + Pf1N1B4_2142 0.58 -0.1 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 +0.8 2,453,628 + Pf1N1B4_2142 0.58 -1.3 2,453,629 - Pf1N1B4_2142 0.58 -0.6 2,453,629 - Pf1N1B4_2142 0.58 -0.8 2,453,629 - Pf1N1B4_2142 0.58 -0.1 2,453,629 - Pf1N1B4_2142 0.58 +0.8 2,453,629 - Pf1N1B4_2142 0.58 -1.7 2,453,629 - Pf1N1B4_2142 0.58 -1.2 2,453,629 - Pf1N1B4_2142 0.58 -4.2 2,453,745 + +1.4 2,453,745 + -1.8 2,453,745 + -2.0 2,453,745 + +0.8 2,453,745 + +1.2 2,453,745 + +0.8 2,453,745 + -3.5 2,453,745 + -0.5 2,453,745 + +0.8 2,453,745 + -0.4 2,453,745 + +0.8 2,453,745 + +1.4 2,453,745 + +0.8 2,453,745 + -2.5 2,453,745 + -1.2 2,453,745 + +0.8 2,453,745 + -1.4 2,453,745 + +0.8 2,453,745 + +0.8 2,453,745 + +0.8 2,453,746 - -2.4 2,453,746 - +0.7 2,453,746 - +0.4 2,453,746 - -0.2 2,453,746 - +0.8 2,453,746 - -2.2 2,453,746 - +0.8 2,453,746 - +0.8 2,453,746 - -0.1 2,453,746 - -1.2 2,453,746 - -0.8 2,453,746 - -0.6 2,453,746 - +0.8 2,453,746 - +2.4 2,453,944 + Pf1N1B4_2143 0.10 -2.5 2,453,944 + Pf1N1B4_2143 0.10 -0.7 2,453,944 + Pf1N1B4_2143 0.10 -0.6 2,453,944 + Pf1N1B4_2143 0.10 +0.5 2,453,944 + Pf1N1B4_2143 0.10 +0.8 2,453,944 + Pf1N1B4_2143 0.10 -1.3 2,453,945 - Pf1N1B4_2143 0.10 -0.9 2,453,945 - Pf1N1B4_2143 0.10 -1.2 2,453,945 - Pf1N1B4_2143 0.10 -0.3 2,453,945 - Pf1N1B4_2143 0.10 -3.0 2,453,945 - Pf1N1B4_2143 0.10 -1.0 2,453,945 - Pf1N1B4_2143 0.10 -2.7 2,453,946 + Pf1N1B4_2143 0.11 -2.0 2,453,946 + Pf1N1B4_2143 0.11 -4.2 2,453,946 + Pf1N1B4_2143 0.11 +0.8 2,453,946 + Pf1N1B4_2143 0.11 -0.7 2,453,946 + Pf1N1B4_2143 0.11 -1.3 2,453,946 + Pf1N1B4_2143 0.11 +0.6 2,453,946 + Pf1N1B4_2143 0.11 +0.8 2,453,947 - Pf1N1B4_2143 0.11 -1.8 2,453,947 - Pf1N1B4_2143 0.11 -1.4 2,453,947 - Pf1N1B4_2143 0.11 -2.7 2,453,947 - Pf1N1B4_2143 0.11 -1.4 2,453,947 - Pf1N1B4_2143 0.11 +0.0 2,453,947 - Pf1N1B4_2143 0.11 -0.1 2,453,947 - Pf1N1B4_2143 0.11 -2.7 2,453,947 - Pf1N1B4_2143 0.11 +0.8 2,453,947 - Pf1N1B4_2143 0.11 -2.2 2,453,947 - Pf1N1B4_2143 0.11 -3.2 2,453,947 - Pf1N1B4_2143 0.11 -0.0 2,453,947 - Pf1N1B4_2143 0.11 -1.5 2,453,947 - Pf1N1B4_2143 0.11 -0.5 2,453,947 - Pf1N1B4_2143 0.11 +0.8 2,453,947 - Pf1N1B4_2143 0.11 +1.2 2,453,947 - Pf1N1B4_2143 0.11 +0.5 2,454,077 + Pf1N1B4_2143 0.37 -4.3 2,454,077 + Pf1N1B4_2143 0.37 -1.2 2,454,077 + Pf1N1B4_2143 0.37 -1.3 2,454,077 + Pf1N1B4_2143 0.37 -3.0 2,454,078 - Pf1N1B4_2143 0.38 +0.8 2,454,078 - Pf1N1B4_2143 0.38 +0.4 2,454,078 - Pf1N1B4_2143 0.38 -2.2 2,454,122 - Pf1N1B4_2143 0.47 +0.3 2,454,122 - Pf1N1B4_2143 0.47 -3.1 2,454,122 - Pf1N1B4_2143 0.47 -1.4 2,454,136 - Pf1N1B4_2143 0.49 -0.6 2,454,169 + Pf1N1B4_2143 0.56 -1.4 2,454,169 + Pf1N1B4_2143 0.56 -3.1 2,454,169 + Pf1N1B4_2143 0.56 -0.6 2,454,169 + Pf1N1B4_2143 0.56 +0.8 2,454,170 - Pf1N1B4_2143 0.56 +0.8 2,454,170 - Pf1N1B4_2143 0.56 +0.8 2,454,170 - Pf1N1B4_2143 0.56 +0.8 2,454,170 - Pf1N1B4_2143 0.56 -0.2 2,454,205 - Pf1N1B4_2143 0.64 +0.8 2,454,383 - +0.4 2,454,443 - -0.2 2,454,443 - -0.2 2,454,444 + -1.2 2,454,445 - -1.1 2,454,473 + -1.8 2,454,502 + -2.4 2,454,503 - -1.9 2,454,503 - -4.3 2,454,533 - -1.2 2,454,587 + -2.4 2,454,587 + -0.7 2,454,587 + -2.0 2,454,587 + -1.6 2,454,588 - -0.8 2,454,588 - -0.0 2,454,590 + -0.8 2,454,590 + -2.8 2,454,590 + -4.5 2,454,590 + -0.4 2,454,590 + -1.3 2,454,590 + -2.2 2,454,590 + +0.8 2,454,590 + -2.3 2,454,590 + -1.7 2,454,590 + -1.8 2,454,689 + Pf1N1B4_2144 0.11 -1.2 2,454,689 + Pf1N1B4_2144 0.11 -2.5
Or see this region's nucleotide sequence