Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1883 and Pf1N1B4_1884 are separated by 12 nucleotides Pf1N1B4_1884 and Pf1N1B4_1885 are separated by 10 nucleotides
Pf1N1B4_1883 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,153,574 to 2,155,190
_1883
Pf1N1B4_1884 - Sensory box histidine kinase, at 2,155,203 to 2,156,993
_1884
Pf1N1B4_1885 - alginate biosynthesis transcriptional regulatory protein AlgB, at 2,157,004 to 2,158,350
_1885
Position (kb)
2155
2156
2157 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2154.244 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.310 kb on + strand, within Pf1N1B4_1883 at 2154.310 kb on + strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.319 kb on + strand, within Pf1N1B4_1883 at 2154.320 kb on - strand, within Pf1N1B4_1883 at 2154.364 kb on + strand, within Pf1N1B4_1883 at 2154.365 kb on - strand, within Pf1N1B4_1883 at 2154.388 kb on + strand, within Pf1N1B4_1883 at 2154.388 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.456 kb on + strand, within Pf1N1B4_1883 at 2154.456 kb on + strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.557 kb on + strand, within Pf1N1B4_1883 at 2154.575 kb on + strand at 2154.610 kb on + strand, within Pf1N1B4_1883 at 2154.611 kb on - strand, within Pf1N1B4_1883 at 2154.611 kb on - strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.876 kb on + strand, within Pf1N1B4_1883 at 2154.877 kb on - strand, within Pf1N1B4_1883 at 2154.891 kb on + strand, within Pf1N1B4_1883 at 2154.939 kb on - strand, within Pf1N1B4_1883 at 2155.164 kb on - strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.206 kb on + strand at 2155.222 kb on + strand at 2155.222 kb on + strand at 2155.222 kb on + strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.252 kb on + strand at 2155.253 kb on - strand at 2155.253 kb on - strand at 2155.375 kb on + strand at 2155.409 kb on + strand, within Pf1N1B4_1884 at 2155.410 kb on - strand at 2155.410 kb on - strand, within Pf1N1B4_1884 at 2155.446 kb on + strand, within Pf1N1B4_1884 at 2155.447 kb on - strand, within Pf1N1B4_1884 at 2155.447 kb on - strand, within Pf1N1B4_1884 at 2155.926 kb on - strand, within Pf1N1B4_1884 at 2155.959 kb on - strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2156.035 kb on - strand, within Pf1N1B4_1884 at 2156.037 kb on + strand, within Pf1N1B4_1884 at 2156.037 kb on + strand, within Pf1N1B4_1884 at 2156.038 kb on - strand, within Pf1N1B4_1884 at 2156.250 kb on - strand, within Pf1N1B4_1884 at 2156.348 kb on + strand at 2156.607 kb on + strand, within Pf1N1B4_1884 at 2156.608 kb on - strand, within Pf1N1B4_1884 at 2156.646 kb on + strand, within Pf1N1B4_1884 at 2156.646 kb on + strand, within Pf1N1B4_1884 at 2156.647 kb on - strand, within Pf1N1B4_1884 at 2156.647 kb on - strand, within Pf1N1B4_1884 at 2156.684 kb on - strand, within Pf1N1B4_1884 at 2156.936 kb on + strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.952 kb on - strand at 2157.014 kb on + strand at 2157.014 kb on + strand at 2157.014 kb on + strand at 2157.015 kb on - strand at 2157.015 kb on - strand at 2157.015 kb on - strand at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.279 kb on - strand, within Pf1N1B4_1885 at 2157.333 kb on - strand, within Pf1N1B4_1885 at 2157.333 kb on - strand, within Pf1N1B4_1885 at 2157.435 kb on - strand, within Pf1N1B4_1885 at 2157.549 kb on - strand, within Pf1N1B4_1885 at 2157.719 kb on - strand, within Pf1N1B4_1885 at 2157.719 kb on - strand, within Pf1N1B4_1885 at 2157.852 kb on + strand, within Pf1N1B4_1885
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 2,154,244 - Pf1N1B4_1883 0.41 -0.1 2,154,271 - Pf1N1B4_1883 0.43 +1.2 2,154,271 - Pf1N1B4_1883 0.43 +0.7 2,154,271 - Pf1N1B4_1883 0.43 -2.9 2,154,310 + Pf1N1B4_1883 0.46 -1.8 2,154,310 + Pf1N1B4_1883 0.46 -2.8 2,154,311 - Pf1N1B4_1883 0.46 -2.6 2,154,311 - Pf1N1B4_1883 0.46 +0.7 2,154,311 - Pf1N1B4_1883 0.46 +0.7 2,154,319 + Pf1N1B4_1883 0.46 -0.3 2,154,320 - Pf1N1B4_1883 0.46 -1.3 2,154,364 + Pf1N1B4_1883 0.49 +0.7 2,154,365 - Pf1N1B4_1883 0.49 +0.3 2,154,388 + Pf1N1B4_1883 0.50 -2.6 2,154,388 + Pf1N1B4_1883 0.50 -2.5 2,154,445 + Pf1N1B4_1883 0.54 -2.6 2,154,445 + Pf1N1B4_1883 0.54 +0.7 2,154,445 + Pf1N1B4_1883 0.54 -0.6 2,154,445 + Pf1N1B4_1883 0.54 -0.1 2,154,446 - Pf1N1B4_1883 0.54 +1.1 2,154,446 - Pf1N1B4_1883 0.54 +0.8 2,154,446 - Pf1N1B4_1883 0.54 +1.0 2,154,446 - Pf1N1B4_1883 0.54 -0.7 2,154,446 - Pf1N1B4_1883 0.54 +0.6 2,154,446 - Pf1N1B4_1883 0.54 +1.1 2,154,446 - Pf1N1B4_1883 0.54 -2.1 2,154,446 - Pf1N1B4_1883 0.54 +0.9 2,154,456 + Pf1N1B4_1883 0.55 -1.3 2,154,456 + Pf1N1B4_1883 0.55 -2.1 2,154,457 - Pf1N1B4_1883 0.55 -3.3 2,154,457 - Pf1N1B4_1883 0.55 -1.7 2,154,457 - Pf1N1B4_1883 0.55 -0.1 2,154,457 - Pf1N1B4_1883 0.55 -2.2 2,154,457 - Pf1N1B4_1883 0.55 -1.1 2,154,457 - Pf1N1B4_1883 0.55 -1.5 2,154,457 - Pf1N1B4_1883 0.55 -0.4 2,154,457 - Pf1N1B4_1883 0.55 +0.7 2,154,557 + Pf1N1B4_1883 0.61 -0.1 2,154,575 + +0.7 2,154,610 + Pf1N1B4_1883 0.64 +0.2 2,154,611 - Pf1N1B4_1883 0.64 -0.7 2,154,611 - Pf1N1B4_1883 0.64 -0.1 2,154,663 + Pf1N1B4_1883 0.67 +0.7 2,154,663 + Pf1N1B4_1883 0.67 -0.2 2,154,663 + Pf1N1B4_1883 0.67 -3.1 2,154,663 + Pf1N1B4_1883 0.67 -1.0 2,154,663 + Pf1N1B4_1883 0.67 -0.1 2,154,663 + Pf1N1B4_1883 0.67 -0.3 2,154,663 + Pf1N1B4_1883 0.67 -0.8 2,154,663 + Pf1N1B4_1883 0.67 -0.3 2,154,663 + Pf1N1B4_1883 0.67 +0.2 2,154,663 + Pf1N1B4_1883 0.67 +1.5 2,154,663 + Pf1N1B4_1883 0.67 -1.6 2,154,663 + Pf1N1B4_1883 0.67 -3.1 2,154,664 - Pf1N1B4_1883 0.67 -2.6 2,154,664 - Pf1N1B4_1883 0.67 -0.3 2,154,664 - Pf1N1B4_1883 0.67 -0.7 2,154,664 - Pf1N1B4_1883 0.67 -2.7 2,154,664 - Pf1N1B4_1883 0.67 +0.1 2,154,664 - Pf1N1B4_1883 0.67 -0.5 2,154,664 - Pf1N1B4_1883 0.67 -1.8 2,154,664 - Pf1N1B4_1883 0.67 -0.1 2,154,664 - Pf1N1B4_1883 0.67 -1.9 2,154,664 - Pf1N1B4_1883 0.67 +1.2 2,154,664 - Pf1N1B4_1883 0.67 -1.9 2,154,664 - Pf1N1B4_1883 0.67 -1.7 2,154,664 - Pf1N1B4_1883 0.67 -2.6 2,154,664 - Pf1N1B4_1883 0.67 -1.9 2,154,664 - Pf1N1B4_1883 0.67 -2.0 2,154,876 + Pf1N1B4_1883 0.81 -1.3 2,154,877 - Pf1N1B4_1883 0.81 +0.2 2,154,891 + Pf1N1B4_1883 0.81 -2.4 2,154,939 - Pf1N1B4_1883 0.84 -2.5 2,155,164 - +0.4 2,155,204 + +0.7 2,155,204 + +0.1 2,155,204 + +0.7 2,155,204 + +0.1 2,155,204 + +0.7 2,155,205 - -2.6 2,155,205 - +1.7 2,155,205 - +0.7 2,155,205 - +0.3 2,155,205 - +0.7 2,155,205 - -3.0 2,155,206 + -2.3 2,155,222 + +0.7 2,155,222 + -0.1 2,155,222 + +0.7 2,155,223 - +0.7 2,155,223 - +0.7 2,155,223 - -0.6 2,155,223 - -1.1 2,155,223 - +0.7 2,155,223 - +0.4 2,155,223 - +0.4 2,155,223 - -1.4 2,155,223 - -1.3 2,155,223 - -0.3 2,155,252 + -1.3 2,155,253 - +0.2 2,155,253 - +0.2 2,155,375 + +0.1 2,155,409 + Pf1N1B4_1884 0.12 +0.7 2,155,410 - -2.1 2,155,410 - Pf1N1B4_1884 0.12 +1.4 2,155,446 + Pf1N1B4_1884 0.14 -0.4 2,155,447 - Pf1N1B4_1884 0.14 +0.7 2,155,447 - Pf1N1B4_1884 0.14 +0.7 2,155,926 - Pf1N1B4_1884 0.40 -3.0 2,155,959 - Pf1N1B4_1884 0.42 +0.7 2,155,960 + Pf1N1B4_1884 0.42 -1.4 2,155,960 + Pf1N1B4_1884 0.42 -2.1 2,155,960 + Pf1N1B4_1884 0.42 +0.2 2,155,960 + Pf1N1B4_1884 0.42 +0.7 2,155,960 + Pf1N1B4_1884 0.42 +1.9 2,155,960 + Pf1N1B4_1884 0.42 -1.7 2,155,960 + Pf1N1B4_1884 0.42 +2.6 2,155,960 + Pf1N1B4_1884 0.42 -1.6 2,155,961 - Pf1N1B4_1884 0.42 +0.7 2,155,961 - Pf1N1B4_1884 0.42 +0.9 2,155,961 - Pf1N1B4_1884 0.42 -2.1 2,155,961 - Pf1N1B4_1884 0.42 +0.8 2,155,961 - Pf1N1B4_1884 0.42 +0.7 2,155,961 - Pf1N1B4_1884 0.42 -0.2 2,155,961 - Pf1N1B4_1884 0.42 +1.3 2,155,961 - Pf1N1B4_1884 0.42 +0.4 2,155,961 - Pf1N1B4_1884 0.42 +0.6 2,155,961 - Pf1N1B4_1884 0.42 -0.2 2,155,961 - Pf1N1B4_1884 0.42 -0.5 2,156,035 - Pf1N1B4_1884 0.46 +0.5 2,156,037 + Pf1N1B4_1884 0.47 -0.4 2,156,037 + Pf1N1B4_1884 0.47 +0.6 2,156,038 - Pf1N1B4_1884 0.47 +0.2 2,156,250 - Pf1N1B4_1884 0.58 +0.7 2,156,348 + +0.7 2,156,607 + Pf1N1B4_1884 0.78 +0.6 2,156,608 - Pf1N1B4_1884 0.78 -2.4 2,156,646 + Pf1N1B4_1884 0.81 -2.1 2,156,646 + Pf1N1B4_1884 0.81 +2.6 2,156,647 - Pf1N1B4_1884 0.81 -0.4 2,156,647 - Pf1N1B4_1884 0.81 -2.4 2,156,684 - Pf1N1B4_1884 0.83 -0.3 2,156,936 + +0.7 2,156,937 - +0.7 2,156,937 - -0.5 2,156,937 - -2.6 2,156,937 - -1.6 2,156,937 - +0.7 2,156,937 - +1.5 2,156,952 - -1.0 2,157,014 + +0.7 2,157,014 + +2.1 2,157,014 + -3.1 2,157,015 - -0.8 2,157,015 - -3.3 2,157,015 - -0.7 2,157,222 + Pf1N1B4_1885 0.16 +0.7 2,157,222 + Pf1N1B4_1885 0.16 -3.2 2,157,222 + Pf1N1B4_1885 0.16 +1.5 2,157,222 + Pf1N1B4_1885 0.16 +0.7 2,157,222 + Pf1N1B4_1885 0.16 +0.7 2,157,222 + Pf1N1B4_1885 0.16 -0.5 2,157,222 + Pf1N1B4_1885 0.16 -0.4 2,157,222 + Pf1N1B4_1885 0.16 +0.2 2,157,223 - Pf1N1B4_1885 0.16 -4.4 2,157,223 - Pf1N1B4_1885 0.16 -0.7 2,157,223 - Pf1N1B4_1885 0.16 -0.1 2,157,223 - Pf1N1B4_1885 0.16 -0.7 2,157,223 - Pf1N1B4_1885 0.16 +0.2 2,157,223 - Pf1N1B4_1885 0.16 +0.6 2,157,223 - Pf1N1B4_1885 0.16 -2.4 2,157,223 - Pf1N1B4_1885 0.16 -0.1 2,157,223 - Pf1N1B4_1885 0.16 -0.4 2,157,223 - Pf1N1B4_1885 0.16 -0.5 2,157,279 - Pf1N1B4_1885 0.20 +0.2 2,157,333 - Pf1N1B4_1885 0.24 -0.2 2,157,333 - Pf1N1B4_1885 0.24 -2.6 2,157,435 - Pf1N1B4_1885 0.32 -0.1 2,157,549 - Pf1N1B4_1885 0.40 +0.5 2,157,719 - Pf1N1B4_1885 0.53 -0.6 2,157,719 - Pf1N1B4_1885 0.53 -0.2 2,157,852 + Pf1N1B4_1885 0.63 +0.7
Or see this region's nucleotide sequence