Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1748 and Pf1N1B4_1749 are separated by 232 nucleotides Pf1N1B4_1749 and Pf1N1B4_1750 overlap by 4 nucleotides Pf1N1B4_1750 and Pf1N1B4_1751 are separated by 3 nucleotides Pf1N1B4_1751 and Pf1N1B4_1752 are separated by 134 nucleotides
Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757
_1748
Pf1N1B4_1749 - Prevent host death protein, Phd antitoxin, at 2,000,990 to 2,001,268
_1749
Pf1N1B4_1750 - Death on curing protein, Doc toxin, at 2,001,265 to 2,001,546
_1750
Pf1N1B4_1751 - FIG00960943: hypothetical protein, at 2,001,550 to 2,001,795
_1751
Pf1N1B4_1752 - FIG00965470: hypothetical protein, at 2,001,930 to 2,002,391
_1752
Position (kb)
2001
2002 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2000.457 kb on - strand, within Pf1N1B4_1748 at 2000.472 kb on - strand, within Pf1N1B4_1748 at 2000.578 kb on + strand, within Pf1N1B4_1748 at 2000.579 kb on - strand, within Pf1N1B4_1748 at 2000.645 kb on - strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.758 kb on + strand at 2000.758 kb on + strand at 2000.759 kb on - strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.829 kb on - strand at 2000.829 kb on - strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.968 kb on + strand at 2000.969 kb on - strand at 2000.969 kb on - strand at 2000.972 kb on + strand at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.272 kb on + strand at 2001.272 kb on + strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.444 kb on - strand, within Pf1N1B4_1750 at 2001.516 kb on + strand, within Pf1N1B4_1750 at 2001.517 kb on - strand at 2001.517 kb on - strand, within Pf1N1B4_1750 at 2001.552 kb on - strand at 2001.720 kb on + strand, within Pf1N1B4_1751 at 2001.721 kb on - strand, within Pf1N1B4_1751 at 2001.817 kb on + strand at 2001.887 kb on + strand at 2001.888 kb on - strand at 2001.931 kb on + strand at 2001.931 kb on + strand at 2002.002 kb on + strand, within Pf1N1B4_1752 at 2002.003 kb on - strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.034 kb on - strand, within Pf1N1B4_1752 at 2002.034 kb on - strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.038 kb on - strand, within Pf1N1B4_1752 at 2002.038 kb on - strand, within Pf1N1B4_1752 at 2002.040 kb on - strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.345 kb on + strand at 2002.392 kb on - strand at 2002.456 kb on + strand at 2002.456 kb on + strand at 2002.457 kb on - strand at 2002.457 kb on - strand at 2002.464 kb on + strand at 2002.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 2,000,457 - Pf1N1B4_1748 0.53 -0.7 2,000,472 - Pf1N1B4_1748 0.55 -0.8 2,000,578 + Pf1N1B4_1748 0.72 -0.1 2,000,579 - Pf1N1B4_1748 0.72 +0.4 2,000,645 - Pf1N1B4_1748 0.82 +1.8 2,000,690 + Pf1N1B4_1748 0.89 -1.6 2,000,690 + Pf1N1B4_1748 0.89 +0.8 2,000,690 + Pf1N1B4_1748 0.89 -0.6 2,000,691 - Pf1N1B4_1748 0.89 -0.5 2,000,691 - Pf1N1B4_1748 0.89 +0.8 2,000,691 - Pf1N1B4_1748 0.89 -3.7 2,000,691 - Pf1N1B4_1748 0.89 +1.5 2,000,691 - Pf1N1B4_1748 0.89 -2.2 2,000,691 - Pf1N1B4_1748 0.89 -0.2 2,000,758 + -0.6 2,000,758 + +0.5 2,000,759 - +0.8 2,000,761 + -1.5 2,000,761 + +0.8 2,000,761 + -2.2 2,000,761 + -2.2 2,000,762 - +0.8 2,000,762 - +0.8 2,000,762 - +0.8 2,000,762 - -1.4 2,000,762 - +0.3 2,000,762 - -2.5 2,000,762 - +0.8 2,000,762 - -4.4 2,000,762 - +0.8 2,000,762 - -0.4 2,000,762 - +0.8 2,000,762 - +0.8 2,000,762 - -3.8 2,000,762 - +0.0 2,000,828 + +0.8 2,000,828 + -0.7 2,000,828 + -2.8 2,000,828 + -2.8 2,000,829 - +0.8 2,000,829 - +0.8 2,000,920 + +0.8 2,000,920 + +0.8 2,000,920 + -1.1 2,000,921 - -0.2 2,000,921 - -3.4 2,000,921 - -1.8 2,000,921 - +0.8 2,000,942 + -2.8 2,000,942 + -2.1 2,000,942 + +0.8 2,000,943 - +0.8 2,000,943 - -1.3 2,000,943 - -1.1 2,000,953 + +1.8 2,000,953 + -1.8 2,000,953 + -1.0 2,000,953 + +0.8 2,000,953 + -0.5 2,000,953 + -1.1 2,000,953 + +0.8 2,000,953 + +0.8 2,000,953 + +1.8 2,000,953 + +0.8 2,000,953 + +0.8 2,000,953 + -0.6 2,000,953 + -1.6 2,000,953 + +0.8 2,000,953 + +0.8 2,000,953 + -1.3 2,000,953 + +0.8 2,000,953 + +0.8 2,000,954 - -3.5 2,000,954 - -0.5 2,000,954 - -1.7 2,000,954 - -1.8 2,000,954 - +0.8 2,000,954 - +0.4 2,000,954 - -1.1 2,000,954 - +0.8 2,000,954 - -2.2 2,000,954 - -0.9 2,000,954 - +0.8 2,000,954 - -3.3 2,000,954 - +1.8 2,000,954 - +0.8 2,000,954 - -1.4 2,000,954 - -2.1 2,000,954 - -2.3 2,000,954 - -2.1 2,000,954 - -0.1 2,000,955 + +0.8 2,000,955 + +0.8 2,000,955 + -1.2 2,000,956 - +0.8 2,000,956 - +0.8 2,000,956 - -3.2 2,000,957 + -0.6 2,000,957 + -2.4 2,000,957 + +0.0 2,000,957 + +0.8 2,000,957 + +0.2 2,000,957 + +0.8 2,000,957 + -0.5 2,000,958 - +0.2 2,000,958 - +1.3 2,000,958 - +0.8 2,000,958 - -1.8 2,000,958 - +0.8 2,000,958 - +0.8 2,000,958 - -3.2 2,000,958 - -0.1 2,000,958 - -0.1 2,000,958 - +0.0 2,000,958 - +0.1 2,000,958 - +0.8 2,000,958 - +0.8 2,000,968 + -0.2 2,000,969 - -2.8 2,000,969 - -0.8 2,000,972 + +0.3 2,001,161 - Pf1N1B4_1749 0.61 -0.6 2,001,161 - Pf1N1B4_1749 0.61 -2.1 2,001,161 - Pf1N1B4_1749 0.61 +2.0 2,001,161 - +0.8 2,001,257 - +0.8 2,001,257 - -1.2 2,001,257 - -1.6 2,001,257 - +0.8 2,001,257 - +0.8 2,001,257 - -2.1 2,001,258 + -1.8 2,001,258 + -1.2 2,001,258 + -1.8 2,001,258 + +0.8 2,001,258 + -0.2 2,001,259 - -0.4 2,001,259 - -1.6 2,001,259 - -0.8 2,001,272 + +0.3 2,001,272 + -1.0 2,001,273 - -0.2 2,001,273 - +0.8 2,001,273 - -2.0 2,001,444 - Pf1N1B4_1750 0.63 +0.8 2,001,516 + Pf1N1B4_1750 0.89 +0.0 2,001,517 - -1.1 2,001,517 - Pf1N1B4_1750 0.89 -3.2 2,001,552 - +0.9 2,001,720 + Pf1N1B4_1751 0.69 +0.2 2,001,721 - Pf1N1B4_1751 0.70 +0.4 2,001,817 + +0.5 2,001,887 + -1.4 2,001,888 - -2.6 2,001,931 + +0.3 2,001,931 + -0.8 2,002,002 + Pf1N1B4_1752 0.16 +0.3 2,002,003 - Pf1N1B4_1752 0.16 +0.5 2,002,033 + Pf1N1B4_1752 0.22 -0.1 2,002,033 + Pf1N1B4_1752 0.22 +0.2 2,002,033 + Pf1N1B4_1752 0.22 +0.7 2,002,033 + Pf1N1B4_1752 0.22 -0.5 2,002,033 + Pf1N1B4_1752 0.22 -0.9 2,002,033 + Pf1N1B4_1752 0.22 -4.2 2,002,034 - Pf1N1B4_1752 0.23 -2.6 2,002,034 - Pf1N1B4_1752 0.23 +0.7 2,002,037 + Pf1N1B4_1752 0.23 -2.7 2,002,037 + Pf1N1B4_1752 0.23 -1.1 2,002,037 + Pf1N1B4_1752 0.23 -0.8 2,002,038 - Pf1N1B4_1752 0.23 +0.7 2,002,038 - Pf1N1B4_1752 0.23 -1.8 2,002,040 - Pf1N1B4_1752 0.24 +0.7 2,002,231 + Pf1N1B4_1752 0.65 -1.0 2,002,231 + Pf1N1B4_1752 0.65 -0.1 2,002,231 + Pf1N1B4_1752 0.65 -1.3 2,002,231 + Pf1N1B4_1752 0.65 -2.6 2,002,231 + +0.7 2,002,231 + Pf1N1B4_1752 0.65 -0.9 2,002,231 + Pf1N1B4_1752 0.65 -0.4 2,002,232 - Pf1N1B4_1752 0.65 +0.7 2,002,232 - Pf1N1B4_1752 0.65 -2.4 2,002,232 - Pf1N1B4_1752 0.65 +0.7 2,002,232 - Pf1N1B4_1752 0.65 +0.7 2,002,345 + +0.7 2,002,392 - +0.2 2,002,456 + -0.3 2,002,456 + -1.6 2,002,457 - +0.6 2,002,457 - -0.8 2,002,464 + -0.2 2,002,464 + +0.7
Or see this region's nucleotide sequence