Experiment: N2E2wt with N1B4m denitrifying repA
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_166 and Pf1N1B4_167 are separated by 24 nucleotides Pf1N1B4_167 and Pf1N1B4_168 are separated by 1 nucleotides Pf1N1B4_168 and Pf1N1B4_169 are separated by 80 nucleotides
Pf1N1B4_166 - Quinohemoprotein amine dehydrogenase beta subunit (EC 1.4.99.-), at 206,350 to 207,468
_166
Pf1N1B4_167 - Quinohemoprotein amine dehydrogenase gamma subunit (EC 1.4.99.-), at 207,493 to 207,816
_167
Pf1N1B4_168 - Arylsulfatase regulator (Fe-S oxidoreductase), at 207,818 to 209,248
_168
Pf1N1B4_169 - Quinohemoprotein amine dehydrogenase 60 kDa subunit, at 209,329 to 210,909
_169
Position (kb) 207
208
209
210 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 206.826 kb on - strand, within Pf1N1B4_166 at 206.826 kb on - strand, within Pf1N1B4_166 at 206.895 kb on - strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.916 kb on - strand, within Pf1N1B4_166 at 206.916 kb on - strand, within Pf1N1B4_166 at 207.012 kb on - strand, within Pf1N1B4_166 at 207.041 kb on + strand, within Pf1N1B4_166 at 207.227 kb on + strand, within Pf1N1B4_166 at 207.228 kb on - strand, within Pf1N1B4_166 at 207.228 kb on - strand, within Pf1N1B4_166 at 207.228 kb on - strand, within Pf1N1B4_166 at 207.362 kb on + strand at 207.363 kb on - strand at 207.418 kb on - strand at 207.630 kb on - strand, within Pf1N1B4_167 at 207.843 kb on + strand at 207.843 kb on + strand at 207.843 kb on + strand at 207.843 kb on + strand at 207.843 kb on + strand at 207.844 kb on - strand at 207.885 kb on + strand at 207.885 kb on + strand at 207.930 kb on + strand at 207.969 kb on + strand, within Pf1N1B4_168 at 207.969 kb on + strand, within Pf1N1B4_168 at 207.969 kb on + strand, within Pf1N1B4_168 at 207.970 kb on - strand at 207.970 kb on - strand, within Pf1N1B4_168 at 207.970 kb on - strand, within Pf1N1B4_168 at 208.533 kb on + strand, within Pf1N1B4_168 at 208.533 kb on + strand, within Pf1N1B4_168 at 208.534 kb on - strand, within Pf1N1B4_168 at 208.731 kb on + strand, within Pf1N1B4_168 at 208.732 kb on - strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.896 kb on + strand, within Pf1N1B4_168 at 208.897 kb on - strand, within Pf1N1B4_168 at 208.897 kb on - strand, within Pf1N1B4_168 at 208.897 kb on - strand, within Pf1N1B4_168 at 209.119 kb on + strand at 209.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA remove 206,826 - Pf1N1B4_166 0.43 -0.3 206,826 - Pf1N1B4_166 0.43 +0.7 206,895 - Pf1N1B4_166 0.49 -3.4 206,915 + Pf1N1B4_166 0.50 +0.1 206,915 + Pf1N1B4_166 0.50 -1.3 206,915 + Pf1N1B4_166 0.50 -2.7 206,916 - Pf1N1B4_166 0.51 -0.8 206,916 - Pf1N1B4_166 0.51 -0.9 207,012 - Pf1N1B4_166 0.59 +0.7 207,041 + Pf1N1B4_166 0.62 -2.9 207,227 + Pf1N1B4_166 0.78 -1.9 207,228 - Pf1N1B4_166 0.78 -0.7 207,228 - Pf1N1B4_166 0.78 +0.1 207,228 - Pf1N1B4_166 0.78 -0.3 207,362 + -2.7 207,363 - -0.3 207,418 - -0.1 207,630 - Pf1N1B4_167 0.42 +0.7 207,843 + -1.6 207,843 + +0.3 207,843 + +0.7 207,843 + +0.7 207,843 + -1.8 207,844 - -1.3 207,885 + +0.6 207,885 + +0.7 207,930 + -2.5 207,969 + Pf1N1B4_168 0.11 +0.6 207,969 + Pf1N1B4_168 0.11 -2.7 207,969 + Pf1N1B4_168 0.11 -0.4 207,970 - +0.7 207,970 - Pf1N1B4_168 0.11 +0.7 207,970 - Pf1N1B4_168 0.11 +1.1 208,533 + Pf1N1B4_168 0.50 -0.5 208,533 + Pf1N1B4_168 0.50 +0.7 208,534 - Pf1N1B4_168 0.50 +0.8 208,731 + Pf1N1B4_168 0.64 -0.4 208,732 - Pf1N1B4_168 0.64 +0.7 208,896 + Pf1N1B4_168 0.75 +0.7 208,896 + Pf1N1B4_168 0.75 -0.4 208,896 + Pf1N1B4_168 0.75 -0.4 208,896 + Pf1N1B4_168 0.75 -1.5 208,896 + Pf1N1B4_168 0.75 -0.5 208,896 + Pf1N1B4_168 0.75 +1.1 208,897 - Pf1N1B4_168 0.75 -0.2 208,897 - Pf1N1B4_168 0.75 +0.5 208,897 - Pf1N1B4_168 0.75 -3.3 209,119 + -2.7 209,240 - -3.1
Or see this region's nucleotide sequence