Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_141

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_140 and Pf1N1B4_141 are separated by 272 nucleotidesPf1N1B4_141 and Pf1N1B4_142 are separated by 210 nucleotides Pf1N1B4_140 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 157,242 to 158,129 _140 Pf1N1B4_141 - PvdE, pyoverdine ABC export system, fused ATPase and permease components, at 158,402 to 160,051 _141 Pf1N1B4_142 - TonB-dependent siderophore receptor precursor, at 160,262 to 162,742 _142 Position (kb) 158 159 160 161Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 157.542 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.677 kb on + strand, within Pf1N1B4_140at 157.743 kb on + strand, within Pf1N1B4_140at 157.744 kb on - strandat 157.831 kb on - strand, within Pf1N1B4_140at 157.848 kb on + strand, within Pf1N1B4_140at 157.921 kb on - strand, within Pf1N1B4_140at 157.921 kb on - strand, within Pf1N1B4_140at 157.953 kb on + strand, within Pf1N1B4_140at 157.953 kb on + strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 157.954 kb on - strand, within Pf1N1B4_140at 158.113 kb on + strandat 158.215 kb on + strandat 158.230 kb on - strandat 158.230 kb on - strandat 158.314 kb on + strandat 158.315 kb on - strandat 158.335 kb on + strandat 158.336 kb on - strandat 158.365 kb on + strandat 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.645 kb on + strand, within Pf1N1B4_141at 158.646 kb on - strand, within Pf1N1B4_141at 158.769 kb on + strand, within Pf1N1B4_141at 158.770 kb on - strand, within Pf1N1B4_141at 158.770 kb on - strand, within Pf1N1B4_141at 158.878 kb on - strand, within Pf1N1B4_141at 158.878 kb on - strand, within Pf1N1B4_141at 159.116 kb on - strand, within Pf1N1B4_141at 159.116 kb on - strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.245 kb on + strand, within Pf1N1B4_141at 159.246 kb on - strand, within Pf1N1B4_141at 159.384 kb on - strand, within Pf1N1B4_141at 159.569 kb on + strand, within Pf1N1B4_141at 159.570 kb on - strand, within Pf1N1B4_141at 159.668 kb on + strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.669 kb on - strand, within Pf1N1B4_141at 159.671 kb on + strand, within Pf1N1B4_141at 159.672 kb on - strand, within Pf1N1B4_141at 159.729 kb on - strand, within Pf1N1B4_141at 160.068 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.145 kb on - strandat 160.239 kb on - strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.382 kb on + strandat 160.478 kb on + strandat 160.478 kb on + strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.479 kb on - strandat 160.851 kb on - strand, within Pf1N1B4_142at 160.874 kb on + strand, within Pf1N1B4_142at 160.874 kb on + strand, within Pf1N1B4_142at 160.949 kb on + strand, within Pf1N1B4_142at 160.949 kb on + strand, within Pf1N1B4_142at 160.950 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142at 160.989 kb on - strand, within Pf1N1B4_142

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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157,542 + Pf1N1B4_140 0.34 -0.3
157,677 + Pf1N1B4_140 0.49 +0.6
157,677 + Pf1N1B4_140 0.49 -0.4
157,677 + Pf1N1B4_140 0.49 -4.2
157,677 + Pf1N1B4_140 0.49 +0.7
157,743 + Pf1N1B4_140 0.56 -3.7
157,744 - -1.4
157,831 - Pf1N1B4_140 0.66 +2.0
157,848 + Pf1N1B4_140 0.68 +0.7
157,921 - Pf1N1B4_140 0.76 -0.9
157,921 - Pf1N1B4_140 0.76 +0.7
157,953 + Pf1N1B4_140 0.80 +1.6
157,953 + Pf1N1B4_140 0.80 +0.7
157,954 - Pf1N1B4_140 0.80 +0.0
157,954 - Pf1N1B4_140 0.80 +0.2
157,954 - Pf1N1B4_140 0.80 -1.9
157,954 - Pf1N1B4_140 0.80 -0.3
158,113 + -2.1
158,215 + -0.5
158,230 - +0.7
158,230 - -3.6
158,314 + +0.3
158,315 - +0.7
158,335 + +0.7
158,336 - -2.6
158,365 + -2.9
158,645 + Pf1N1B4_141 0.15 +0.7
158,645 + Pf1N1B4_141 0.15 -3.2
158,645 + Pf1N1B4_141 0.15 +1.2
158,645 + Pf1N1B4_141 0.15 -3.0
158,646 - Pf1N1B4_141 0.15 +1.0
158,769 + Pf1N1B4_141 0.22 -2.3
158,770 - Pf1N1B4_141 0.22 -2.7
158,770 - Pf1N1B4_141 0.22 -0.8
158,878 - Pf1N1B4_141 0.29 +0.7
158,878 - Pf1N1B4_141 0.29 -0.9
159,116 - Pf1N1B4_141 0.43 -3.1
159,116 - Pf1N1B4_141 0.43 +1.9
159,245 + Pf1N1B4_141 0.51 -0.4
159,245 + Pf1N1B4_141 0.51 +1.2
159,245 + Pf1N1B4_141 0.51 -0.5
159,246 - Pf1N1B4_141 0.51 -1.2
159,384 - Pf1N1B4_141 0.60 +0.7
159,569 + Pf1N1B4_141 0.71 -2.9
159,570 - Pf1N1B4_141 0.71 -1.2
159,668 + Pf1N1B4_141 0.77 -2.1
159,669 - Pf1N1B4_141 0.77 +0.7
159,669 - Pf1N1B4_141 0.77 +0.4
159,669 - Pf1N1B4_141 0.77 -0.5
159,671 + Pf1N1B4_141 0.77 -0.7
159,672 - Pf1N1B4_141 0.77 -1.6
159,729 - Pf1N1B4_141 0.80 -0.8
160,068 - -2.1
160,145 - -2.9
160,145 - -0.9
160,145 - +0.3
160,145 - -2.7
160,239 - +0.5
160,382 + +0.7
160,382 + -1.6
160,382 + -1.1
160,382 + +0.7
160,478 + +0.7
160,478 + -0.8
160,479 - -2.5
160,479 - +0.7
160,479 - -1.1
160,479 - +0.7
160,851 - Pf1N1B4_142 0.24 -0.1
160,874 + Pf1N1B4_142 0.25 +0.4
160,874 + Pf1N1B4_142 0.25 +0.3
160,949 + Pf1N1B4_142 0.28 -3.2
160,949 + Pf1N1B4_142 0.28 -3.5
160,950 - Pf1N1B4_142 0.28 -0.3
160,989 - Pf1N1B4_142 0.29 -3.1
160,989 - Pf1N1B4_142 0.29 -0.5
160,989 - Pf1N1B4_142 0.29 -0.5

Or see this region's nucleotide sequence