Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_102

Experiment: N2E2wt with N1B4m denitrifying repA

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotidesPf1N1B4_101 and Pf1N1B4_102 are separated by 374 nucleotidesPf1N1B4_102 and Pf1N1B4_103 are separated by 108 nucleotides Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082 _100 Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311 _101 Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446 _102 Pf1N1B4_103 - Histidine permease YuiF, at 108,555 to 109,871 _103 Position (kb) 106 107 108 109Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 105.791 kb on - strandat 105.828 kb on - strandat 105.828 kb on - strandat 105.829 kb on + strandat 105.830 kb on - strandat 105.830 kb on - strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.845 kb on + strandat 105.846 kb on - strandat 105.854 kb on + strandat 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.886 kb on - strand, within Pf1N1B4_100at 106.226 kb on + strand, within Pf1N1B4_101at 106.226 kb on + strand, within Pf1N1B4_101at 106.227 kb on - strand, within Pf1N1B4_101at 106.293 kb on + strand, within Pf1N1B4_101at 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.428 kb on - strandat 106.428 kb on - strandat 106.501 kb on + strandat 106.502 kb on - strandat 106.549 kb on + strandat 106.550 kb on - strandat 106.677 kb on + strandat 107.716 kb on - strand, within Pf1N1B4_102at 107.921 kb on - strand, within Pf1N1B4_102at 107.974 kb on + strand, within Pf1N1B4_102at 108.091 kb on + strand, within Pf1N1B4_102at 108.196 kb on + strand, within Pf1N1B4_102at 108.408 kb on + strandat 108.409 kb on - strandat 108.409 kb on - strandat 108.409 kb on - strandat 108.495 kb on + strandat 108.495 kb on + strandat 108.556 kb on + strandat 108.557 kb on - strandat 108.557 kb on - strandat 108.605 kb on - strandat 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strand, within Pf1N1B4_103at 109.175 kb on - strandat 109.219 kb on + strand, within Pf1N1B4_103at 109.324 kb on - strand, within Pf1N1B4_103at 109.399 kb on + strand, within Pf1N1B4_103at 109.399 kb on + strand, within Pf1N1B4_103at 109.399 kb on + strand, within Pf1N1B4_103at 109.400 kb on - strand, within Pf1N1B4_103

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Per-strain Table

Position Strand Gene LocusTag Fraction N2E2wt with N1B4m denitrifying repA
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105,791 - +0.5
105,828 - -0.3
105,828 - -3.5
105,829 + -2.5
105,830 - -1.2
105,830 - -0.1
105,833 + +0.7
105,833 + +0.7
105,833 + -2.6
105,834 - +0.6
105,834 - +1.2
105,834 - +1.6
105,834 - -0.9
105,834 - -0.3
105,834 - -0.3
105,834 - -0.4
105,834 - +0.1
105,845 + -2.9
105,846 - -3.7
105,854 + -0.0
105,885 + Pf1N1B4_100 0.13 -0.8
105,885 + Pf1N1B4_100 0.13 +0.1
105,885 + Pf1N1B4_100 0.13 -2.6
105,886 - Pf1N1B4_100 0.14 +0.7
106,226 + Pf1N1B4_101 0.48 -2.1
106,226 + Pf1N1B4_101 0.48 +0.1
106,227 - Pf1N1B4_101 0.48 +0.8
106,293 + Pf1N1B4_101 0.88 +0.7
106,424 + -0.9
106,424 + +0.4
106,424 + -1.6
106,424 + -0.5
106,428 - -0.3
106,428 - -0.5
106,501 + -1.4
106,502 - +0.7
106,549 + +0.7
106,550 - +1.5
106,677 + -0.8
107,716 - Pf1N1B4_102 0.58 -1.2
107,921 - Pf1N1B4_102 0.70 -2.8
107,974 + Pf1N1B4_102 0.73 -0.3
108,091 + Pf1N1B4_102 0.80 -3.2
108,196 + Pf1N1B4_102 0.86 +0.7
108,408 + -0.7
108,409 - -1.6
108,409 - -0.3
108,409 - -0.3
108,495 + +0.7
108,495 + +0.7
108,556 + -2.6
108,557 - -1.1
108,557 - -0.9
108,605 - -0.9
109,175 - Pf1N1B4_103 0.47 +0.7
109,175 - Pf1N1B4_103 0.47 -1.9
109,175 - Pf1N1B4_103 0.47 +0.1
109,175 - Pf1N1B4_103 0.47 +1.3
109,175 - Pf1N1B4_103 0.47 +0.7
109,175 - Pf1N1B4_103 0.47 -2.5
109,175 - Pf1N1B4_103 0.47 -2.1
109,175 - Pf1N1B4_103 0.47 +0.7
109,175 - Pf1N1B4_103 0.47 -1.6
109,175 - Pf1N1B4_103 0.47 +0.7
109,175 - -4.2
109,219 + Pf1N1B4_103 0.50 -3.3
109,324 - Pf1N1B4_103 0.58 +0.2
109,399 + Pf1N1B4_103 0.64 +0.5
109,399 + Pf1N1B4_103 0.64 -0.7
109,399 + Pf1N1B4_103 0.64 -1.6
109,400 - Pf1N1B4_103 0.64 +0.7

Or see this region's nucleotide sequence