Experiment: reconstituted MRS with Sialyllacto-N-tetraose d 5.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18150 and BBR_RS18155 are separated by 216 nucleotides BBR_RS18155 and BBR_RS18160 are separated by 40 nucleotides BBR_RS18160 and BBR_RS18165 are separated by 94 nucleotides
BBR_RS18150: BBR_RS18150 - energy-dependent translational throttle protein EttA, at 1,857,003 to 1,858,682
_RS18150
BBR_RS18155: BBR_RS18155 - acyl-CoA thioesterase II, at 1,858,899 to 1,859,804
_RS18155
BBR_RS18160: BBR_RS18160 - DUF3180 domain-containing protein, at 1,859,845 to 1,860,372
_RS18160
BBR_RS18165: BBR_RS18165 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,860,467 to 1,862,077
_RS18165
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-2
-1
0
1 at 1858.686 kb on - strand at 1858.691 kb on - strand at 1858.761 kb on - strand at 1858.761 kb on - strand at 1858.761 kb on - strand at 1858.761 kb on - strand at 1858.763 kb on + strand at 1858.763 kb on + strand at 1858.763 kb on + strand at 1858.771 kb on - strand at 1858.771 kb on - strand at 1858.771 kb on - strand at 1858.771 kb on - strand at 1858.818 kb on + strand at 1858.818 kb on + strand at 1858.826 kb on - strand at 1858.826 kb on - strand at 1858.826 kb on - strand at 1858.889 kb on + strand at 1858.895 kb on + strand at 1858.895 kb on + strand at 1858.897 kb on + strand at 1858.900 kb on - strand at 1858.903 kb on - strand at 1858.923 kb on + strand at 1858.937 kb on + strand at 1858.945 kb on - strand at 1858.945 kb on - strand at 1858.946 kb on + strand at 1858.961 kb on + strand at 1858.961 kb on + strand at 1858.969 kb on - strand at 1858.969 kb on - strand at 1858.969 kb on - strand at 1858.990 kb on - strand, within BBR_RS18155 at 1859.024 kb on + strand, within BBR_RS18155 at 1859.074 kb on - strand, within BBR_RS18155 at 1859.074 kb on - strand, within BBR_RS18155 at 1859.075 kb on + strand, within BBR_RS18155 at 1859.095 kb on - strand, within BBR_RS18155 at 1859.111 kb on - strand, within BBR_RS18155 at 1859.153 kb on - strand, within BBR_RS18155 at 1859.153 kb on - strand, within BBR_RS18155 at 1859.158 kb on + strand, within BBR_RS18155 at 1859.268 kb on + strand, within BBR_RS18155 at 1859.273 kb on + strand, within BBR_RS18155 at 1859.292 kb on + strand, within BBR_RS18155 at 1859.298 kb on + strand, within BBR_RS18155 at 1859.323 kb on + strand, within BBR_RS18155 at 1859.337 kb on - strand, within BBR_RS18155 at 1859.418 kb on - strand, within BBR_RS18155 at 1859.429 kb on + strand, within BBR_RS18155 at 1859.432 kb on - strand, within BBR_RS18155 at 1859.444 kb on + strand, within BBR_RS18155 at 1859.447 kb on - strand, within BBR_RS18155 at 1859.452 kb on - strand, within BBR_RS18155 at 1859.480 kb on - strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.517 kb on + strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.525 kb on - strand, within BBR_RS18155 at 1859.535 kb on - strand, within BBR_RS18155 at 1859.565 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.571 kb on + strand, within BBR_RS18155 at 1859.631 kb on + strand, within BBR_RS18155 at 1859.682 kb on + strand, within BBR_RS18155 at 1859.682 kb on + strand, within BBR_RS18155 at 1859.690 kb on - strand, within BBR_RS18155 at 1859.690 kb on - strand, within BBR_RS18155 at 1859.710 kb on + strand, within BBR_RS18155 at 1859.718 kb on - strand at 1859.718 kb on - strand at 1859.720 kb on + strand at 1859.725 kb on + strand at 1859.728 kb on - strand at 1859.733 kb on + strand at 1859.778 kb on + strand at 1859.778 kb on + strand at 1859.778 kb on + strand at 1859.782 kb on - strand at 1859.786 kb on - strand at 1859.812 kb on + strand at 1859.843 kb on + strand at 1859.860 kb on - strand at 1859.865 kb on - strand at 1859.905 kb on + strand, within BBR_RS18160 at 1859.912 kb on + strand, within BBR_RS18160 at 1859.913 kb on - strand, within BBR_RS18160 at 1859.913 kb on - strand, within BBR_RS18160 at 1859.962 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.974 kb on + strand, within BBR_RS18160 at 1859.979 kb on + strand, within BBR_RS18160 at 1859.987 kb on - strand, within BBR_RS18160 at 1860.010 kb on - strand, within BBR_RS18160 at 1860.010 kb on - strand, within BBR_RS18160 at 1860.012 kb on + strand, within BBR_RS18160 at 1860.014 kb on + strand, within BBR_RS18160 at 1860.014 kb on + strand, within BBR_RS18160 at 1860.017 kb on - strand, within BBR_RS18160 at 1860.022 kb on - strand, within BBR_RS18160 at 1860.022 kb on - strand, within BBR_RS18160 at 1860.036 kb on + strand, within BBR_RS18160 at 1860.118 kb on + strand, within BBR_RS18160 at 1860.135 kb on - strand, within BBR_RS18160 at 1860.184 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.200 kb on + strand, within BBR_RS18160 at 1860.201 kb on - strand, within BBR_RS18160 at 1860.210 kb on + strand, within BBR_RS18160 at 1860.215 kb on - strand, within BBR_RS18160 at 1860.220 kb on + strand, within BBR_RS18160 at 1860.223 kb on - strand, within BBR_RS18160 at 1860.228 kb on - strand, within BBR_RS18160 at 1860.300 kb on + strand, within BBR_RS18160 at 1860.302 kb on - strand, within BBR_RS18160 at 1860.324 kb on + strand at 1860.324 kb on + strand at 1860.334 kb on + strand at 1860.439 kb on + strand at 1860.499 kb on - strand at 1860.535 kb on + strand at 1860.584 kb on - strand at 1860.657 kb on - strand, within BBR_RS18165 at 1860.657 kb on - strand, within BBR_RS18165 at 1860.663 kb on + strand, within BBR_RS18165 at 1860.665 kb on - strand, within BBR_RS18165 at 1860.671 kb on - strand, within BBR_RS18165 at 1860.673 kb on - strand, within BBR_RS18165 at 1860.744 kb on - strand, within BBR_RS18165 at 1860.756 kb on + strand, within BBR_RS18165 at 1860.793 kb on - strand, within BBR_RS18165
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Sialyllacto-N-tetraose d 5.0 mM remove 1,858,686 - -0.1 1,858,691 - -0.1 1,858,761 - +0.2 1,858,761 - +0.6 1,858,761 - -0.2 1,858,761 - -0.7 1,858,763 + +0.1 1,858,763 + +0.6 1,858,763 + +0.4 1,858,771 - +0.5 1,858,771 - -0.1 1,858,771 - +0.5 1,858,771 - -0.9 1,858,818 + -0.9 1,858,818 + -0.7 1,858,826 - -0.1 1,858,826 - -0.2 1,858,826 - +1.0 1,858,889 + -0.7 1,858,895 + +0.6 1,858,895 + +0.8 1,858,897 + +0.3 1,858,900 - -1.9 1,858,903 - -0.3 1,858,923 + +0.6 1,858,937 + -0.1 1,858,945 - -0.9 1,858,945 - +0.2 1,858,946 + +0.3 1,858,961 + -0.1 1,858,961 + -1.3 1,858,969 - -0.0 1,858,969 - -0.5 1,858,969 - +1.6 1,858,990 - BBR_RS18155 0.10 -0.5 1,859,024 + BBR_RS18155 0.14 -0.0 1,859,074 - BBR_RS18155 0.19 -2.1 1,859,074 - BBR_RS18155 0.19 +0.1 1,859,075 + BBR_RS18155 0.19 +1.0 1,859,095 - BBR_RS18155 0.22 -0.1 1,859,111 - BBR_RS18155 0.23 +0.6 1,859,153 - BBR_RS18155 0.28 -0.3 1,859,153 - BBR_RS18155 0.28 -0.3 1,859,158 + BBR_RS18155 0.29 -0.3 1,859,268 + BBR_RS18155 0.41 -1.0 1,859,273 + BBR_RS18155 0.41 -1.3 1,859,292 + BBR_RS18155 0.43 -0.9 1,859,298 + BBR_RS18155 0.44 -0.7 1,859,323 + BBR_RS18155 0.47 +0.3 1,859,337 - BBR_RS18155 0.48 -0.9 1,859,418 - BBR_RS18155 0.57 +0.7 1,859,429 + BBR_RS18155 0.58 +0.1 1,859,432 - BBR_RS18155 0.59 -0.2 1,859,444 + BBR_RS18155 0.60 +0.1 1,859,447 - BBR_RS18155 0.60 -1.2 1,859,452 - BBR_RS18155 0.61 +0.1 1,859,480 - BBR_RS18155 0.64 -0.2 1,859,517 + BBR_RS18155 0.68 +1.0 1,859,517 + BBR_RS18155 0.68 -0.4 1,859,517 + BBR_RS18155 0.68 -0.0 1,859,517 + BBR_RS18155 0.68 -0.1 1,859,525 - BBR_RS18155 0.69 +0.5 1,859,525 - BBR_RS18155 0.69 -0.4 1,859,525 - BBR_RS18155 0.69 +0.4 1,859,535 - BBR_RS18155 0.70 +0.4 1,859,565 + BBR_RS18155 0.74 +0.2 1,859,571 + BBR_RS18155 0.74 -1.1 1,859,571 + BBR_RS18155 0.74 -0.7 1,859,571 + BBR_RS18155 0.74 -1.1 1,859,631 + BBR_RS18155 0.81 -0.1 1,859,682 + BBR_RS18155 0.86 -0.4 1,859,682 + BBR_RS18155 0.86 -0.0 1,859,690 - BBR_RS18155 0.87 -0.5 1,859,690 - BBR_RS18155 0.87 +0.3 1,859,710 + BBR_RS18155 0.90 +0.4 1,859,718 - -0.5 1,859,718 - -0.1 1,859,720 + -0.6 1,859,725 + +0.3 1,859,728 - -0.2 1,859,733 + -0.0 1,859,778 + +0.2 1,859,778 + -0.4 1,859,778 + -0.5 1,859,782 - -0.3 1,859,786 - -0.3 1,859,812 + -0.4 1,859,843 + +0.1 1,859,860 - -1.2 1,859,865 - -0.1 1,859,905 + BBR_RS18160 0.11 +0.4 1,859,912 + BBR_RS18160 0.13 +0.5 1,859,913 - BBR_RS18160 0.13 +0.6 1,859,913 - BBR_RS18160 0.13 +1.1 1,859,962 + BBR_RS18160 0.22 +0.5 1,859,974 + BBR_RS18160 0.24 -0.2 1,859,974 + BBR_RS18160 0.24 +0.5 1,859,974 + BBR_RS18160 0.24 +0.7 1,859,979 + BBR_RS18160 0.25 +0.6 1,859,987 - BBR_RS18160 0.27 +0.4 1,860,010 - BBR_RS18160 0.31 -1.3 1,860,010 - BBR_RS18160 0.31 -0.2 1,860,012 + BBR_RS18160 0.32 +0.3 1,860,014 + BBR_RS18160 0.32 +0.8 1,860,014 + BBR_RS18160 0.32 -0.2 1,860,017 - BBR_RS18160 0.33 -0.3 1,860,022 - BBR_RS18160 0.34 +0.1 1,860,022 - BBR_RS18160 0.34 +0.4 1,860,036 + BBR_RS18160 0.36 -1.1 1,860,118 + BBR_RS18160 0.52 +1.1 1,860,135 - BBR_RS18160 0.55 +0.1 1,860,184 + BBR_RS18160 0.64 +0.3 1,860,200 + BBR_RS18160 0.67 -0.6 1,860,200 + BBR_RS18160 0.67 -0.6 1,860,200 + BBR_RS18160 0.67 -0.2 1,860,200 + BBR_RS18160 0.67 -0.3 1,860,201 - BBR_RS18160 0.67 -0.1 1,860,210 + BBR_RS18160 0.69 -0.3 1,860,215 - BBR_RS18160 0.70 +0.0 1,860,220 + BBR_RS18160 0.71 +0.1 1,860,223 - BBR_RS18160 0.72 -0.7 1,860,228 - BBR_RS18160 0.73 -0.0 1,860,300 + BBR_RS18160 0.86 +0.4 1,860,302 - BBR_RS18160 0.87 +0.2 1,860,324 + +0.2 1,860,324 + -0.6 1,860,334 + -0.5 1,860,439 + +0.4 1,860,499 - -0.1 1,860,535 + +0.3 1,860,584 - -0.8 1,860,657 - BBR_RS18165 0.12 -0.9 1,860,657 - BBR_RS18165 0.12 -0.6 1,860,663 + BBR_RS18165 0.12 -0.0 1,860,665 - BBR_RS18165 0.12 +0.2 1,860,671 - BBR_RS18165 0.13 -0.5 1,860,673 - BBR_RS18165 0.13 -0.8 1,860,744 - BBR_RS18165 0.17 +0.4 1,860,756 + BBR_RS18165 0.18 +1.7 1,860,793 - BBR_RS18165 0.20 +0.1
Or see this region's nucleotide sequence