Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_27450 and AO356_27455 are separated by 120 nucleotides AO356_27455 and AO356_27460 are separated by 123 nucleotides
AO356_27450: AO356_27450 - fumarylacetoacetate hydrolase, at 6,254,616 to 6,255,794
_27450
AO356_27455: AO356_27455 - LysR family transcriptional regulator, at 6,255,915 to 6,256,901
_27455
AO356_27460: AO356_27460 - galactonate dehydratase, at 6,257,025 to 6,258,572
_27460
Position (kb)
6255
6256
6257 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6254.984 kb on + strand, within AO356_27450 at 6254.985 kb on - strand, within AO356_27450 at 6254.985 kb on - strand, within AO356_27450 at 6255.290 kb on + strand, within AO356_27450 at 6255.290 kb on + strand, within AO356_27450 at 6255.290 kb on + strand, within AO356_27450 at 6255.291 kb on - strand, within AO356_27450 at 6255.291 kb on - strand, within AO356_27450 at 6255.291 kb on - strand, within AO356_27450 at 6255.574 kb on + strand, within AO356_27450 at 6255.833 kb on + strand at 6255.932 kb on + strand at 6255.933 kb on - strand at 6255.933 kb on - strand at 6255.986 kb on + strand at 6255.986 kb on + strand at 6255.986 kb on + strand at 6255.987 kb on - strand at 6255.987 kb on - strand at 6255.987 kb on - strand at 6255.987 kb on - strand at 6256.056 kb on - strand, within AO356_27455 at 6256.056 kb on - strand, within AO356_27455 at 6256.148 kb on - strand, within AO356_27455 at 6256.164 kb on - strand, within AO356_27455 at 6256.234 kb on + strand, within AO356_27455 at 6256.235 kb on - strand, within AO356_27455 at 6256.539 kb on + strand, within AO356_27455 at 6256.540 kb on - strand, within AO356_27455 at 6256.540 kb on - strand, within AO356_27455 at 6256.540 kb on - strand, within AO356_27455 at 6256.764 kb on + strand, within AO356_27455 at 6256.772 kb on + strand, within AO356_27455 at 6256.773 kb on - strand, within AO356_27455 at 6256.817 kb on + strand at 6256.817 kb on + strand at 6256.934 kb on + strand at 6256.957 kb on + strand at 6256.958 kb on - strand at 6256.987 kb on + strand at 6257.172 kb on - strand at 6257.251 kb on + strand, within AO356_27460 at 6257.251 kb on + strand, within AO356_27460 at 6257.252 kb on - strand, within AO356_27460 at 6257.409 kb on + strand, within AO356_27460 at 6257.409 kb on + strand, within AO356_27460 at 6257.409 kb on + strand, within AO356_27460 at 6257.410 kb on - strand, within AO356_27460 at 6257.418 kb on - strand, within AO356_27460 at 6257.418 kb on - strand, within AO356_27460 at 6257.424 kb on - strand, within AO356_27460 at 6257.424 kb on - strand, within AO356_27460 at 6257.424 kb on - strand, within AO356_27460 at 6257.508 kb on + strand, within AO356_27460
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,254,984 + AO356_27450 0.31 +1.5 6,254,985 - AO356_27450 0.31 +1.5 6,254,985 - AO356_27450 0.31 -0.1 6,255,290 + AO356_27450 0.57 -1.7 6,255,290 + AO356_27450 0.57 +1.9 6,255,290 + AO356_27450 0.57 -2.2 6,255,291 - AO356_27450 0.57 -1.3 6,255,291 - AO356_27450 0.57 +0.7 6,255,291 - AO356_27450 0.57 +2.1 6,255,574 + AO356_27450 0.81 +0.1 6,255,833 + +0.6 6,255,932 + +0.3 6,255,933 - -1.8 6,255,933 - +1.5 6,255,986 + -1.9 6,255,986 + -1.8 6,255,986 + +0.6 6,255,987 - -0.1 6,255,987 - +0.6 6,255,987 - -0.2 6,255,987 - +0.9 6,256,056 - AO356_27455 0.14 +2.3 6,256,056 - AO356_27455 0.14 -1.4 6,256,148 - AO356_27455 0.24 +0.2 6,256,164 - AO356_27455 0.25 +0.9 6,256,234 + AO356_27455 0.32 -1.3 6,256,235 - AO356_27455 0.32 +0.3 6,256,539 + AO356_27455 0.63 +1.9 6,256,540 - AO356_27455 0.63 -0.1 6,256,540 - AO356_27455 0.63 -1.6 6,256,540 - AO356_27455 0.63 +0.2 6,256,764 + AO356_27455 0.86 -1.7 6,256,772 + AO356_27455 0.87 -1.4 6,256,773 - AO356_27455 0.87 -0.5 6,256,817 + -1.7 6,256,817 + -0.5 6,256,934 + -2.2 6,256,957 + +0.0 6,256,958 - -0.4 6,256,987 + +0.7 6,257,172 - +0.6 6,257,251 + AO356_27460 0.15 +0.8 6,257,251 + AO356_27460 0.15 +1.0 6,257,252 - AO356_27460 0.15 -0.4 6,257,409 + AO356_27460 0.25 +0.2 6,257,409 + AO356_27460 0.25 +0.1 6,257,409 + AO356_27460 0.25 +1.0 6,257,410 - AO356_27460 0.25 +1.6 6,257,418 - AO356_27460 0.25 +2.5 6,257,418 - AO356_27460 0.25 +1.0 6,257,424 - AO356_27460 0.26 +0.2 6,257,424 - AO356_27460 0.26 -0.6 6,257,424 - AO356_27460 0.26 +0.3 6,257,508 + AO356_27460 0.31 -2.0
Or see this region's nucleotide sequence