Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_26965 and AO356_26970 are separated by 192 nucleotides AO356_26970 and AO356_26975 are separated by 12 nucleotides AO356_26975 and AO356_26980 are separated by 153 nucleotides
AO356_26965: AO356_26965 - hypothetical protein, at 6,140,126 to 6,141,511
_26965
AO356_26970: AO356_26970 - sodium:proton antiporter, at 6,141,704 to 6,143,041
_26970
AO356_26975: AO356_26975 - hypothetical protein, at 6,143,054 to 6,143,590
_26975
AO356_26980: AO356_26980 - amino acid transporter, at 6,143,744 to 6,143,974
_26980
Position (kb)
6141
6142
6143
6144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6140.826 kb on + strand, within AO356_26965 at 6140.826 kb on + strand, within AO356_26965 at 6140.826 kb on + strand, within AO356_26965 at 6140.826 kb on + strand, within AO356_26965 at 6140.827 kb on - strand, within AO356_26965 at 6140.827 kb on - strand, within AO356_26965 at 6140.829 kb on + strand, within AO356_26965 at 6140.829 kb on + strand, within AO356_26965 at 6140.859 kb on + strand, within AO356_26965 at 6140.928 kb on + strand, within AO356_26965 at 6140.928 kb on + strand, within AO356_26965 at 6140.929 kb on - strand, within AO356_26965 at 6140.943 kb on + strand, within AO356_26965 at 6141.087 kb on + strand, within AO356_26965 at 6141.088 kb on - strand, within AO356_26965 at 6141.088 kb on - strand, within AO356_26965 at 6141.142 kb on - strand, within AO356_26965 at 6141.193 kb on - strand, within AO356_26965 at 6141.193 kb on - strand, within AO356_26965 at 6141.256 kb on - strand, within AO356_26965 at 6141.264 kb on + strand, within AO356_26965 at 6141.433 kb on - strand at 6141.455 kb on - strand at 6141.511 kb on + strand at 6141.511 kb on + strand at 6141.511 kb on + strand at 6141.512 kb on - strand at 6141.668 kb on + strand at 6141.717 kb on + strand at 6141.718 kb on - strand at 6141.771 kb on - strand at 6141.964 kb on + strand, within AO356_26970 at 6142.040 kb on + strand, within AO356_26970 at 6142.041 kb on - strand, within AO356_26970 at 6142.041 kb on - strand, within AO356_26970 at 6142.397 kb on + strand, within AO356_26970 at 6142.398 kb on - strand, within AO356_26970 at 6142.428 kb on - strand, within AO356_26970 at 6142.886 kb on + strand, within AO356_26970 at 6142.887 kb on - strand, within AO356_26970 at 6142.887 kb on - strand, within AO356_26970 at 6143.015 kb on + strand at 6143.015 kb on + strand at 6143.015 kb on + strand at 6143.015 kb on + strand at 6143.016 kb on - strand at 6143.016 kb on - strand at 6143.016 kb on - strand at 6143.016 kb on - strand at 6143.016 kb on - strand at 6143.082 kb on + strand at 6143.082 kb on + strand at 6143.083 kb on - strand at 6143.083 kb on - strand at 6143.083 kb on - strand at 6143.442 kb on + strand, within AO356_26975 at 6143.443 kb on - strand, within AO356_26975 at 6143.479 kb on - strand, within AO356_26975 at 6143.496 kb on + strand, within AO356_26975 at 6143.496 kb on + strand, within AO356_26975 at 6143.497 kb on - strand, within AO356_26975 at 6143.717 kb on - strand at 6143.717 kb on - strand at 6143.717 kb on - strand at 6143.717 kb on - strand at 6143.722 kb on + strand at 6143.854 kb on + strand, within AO356_26980 at 6143.866 kb on + strand, within AO356_26980 at 6143.866 kb on + strand, within AO356_26980 at 6143.867 kb on - strand, within AO356_26980 at 6143.894 kb on - strand, within AO356_26980 at 6143.903 kb on + strand, within AO356_26980 at 6143.904 kb on - strand, within AO356_26980 at 6143.904 kb on - strand, within AO356_26980 at 6143.904 kb on - strand, within AO356_26980 at 6143.904 kb on - strand, within AO356_26980 at 6143.972 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 6,140,826 + AO356_26965 0.51 +1.2 6,140,826 + AO356_26965 0.51 -1.7 6,140,826 + AO356_26965 0.51 -1.7 6,140,826 + AO356_26965 0.51 -0.5 6,140,827 - AO356_26965 0.51 +1.0 6,140,827 - AO356_26965 0.51 -0.8 6,140,829 + AO356_26965 0.51 -0.6 6,140,829 + AO356_26965 0.51 +1.5 6,140,859 + AO356_26965 0.53 -2.2 6,140,928 + AO356_26965 0.58 -1.1 6,140,928 + AO356_26965 0.58 +0.8 6,140,929 - AO356_26965 0.58 -1.6 6,140,943 + AO356_26965 0.59 +1.3 6,141,087 + AO356_26965 0.69 +1.2 6,141,088 - AO356_26965 0.69 +0.2 6,141,088 - AO356_26965 0.69 +0.5 6,141,142 - AO356_26965 0.73 +1.2 6,141,193 - AO356_26965 0.77 +0.4 6,141,193 - AO356_26965 0.77 -1.6 6,141,256 - AO356_26965 0.82 -0.7 6,141,264 + AO356_26965 0.82 +1.3 6,141,433 - -0.3 6,141,455 - -0.3 6,141,511 + +0.9 6,141,511 + -1.7 6,141,511 + -1.2 6,141,512 - +1.6 6,141,668 + +1.5 6,141,717 + -1.2 6,141,718 - -0.2 6,141,771 - +0.2 6,141,964 + AO356_26970 0.19 +0.2 6,142,040 + AO356_26970 0.25 +0.1 6,142,041 - AO356_26970 0.25 -0.2 6,142,041 - AO356_26970 0.25 -0.9 6,142,397 + AO356_26970 0.52 -1.7 6,142,398 - AO356_26970 0.52 +0.4 6,142,428 - AO356_26970 0.54 +0.0 6,142,886 + AO356_26970 0.88 +1.2 6,142,887 - AO356_26970 0.88 -1.1 6,142,887 - AO356_26970 0.88 -1.1 6,143,015 + +0.9 6,143,015 + +1.6 6,143,015 + +1.3 6,143,015 + -1.3 6,143,016 - +1.8 6,143,016 - -0.6 6,143,016 - +0.5 6,143,016 - +2.6 6,143,016 - +0.9 6,143,082 + -0.5 6,143,082 + -1.3 6,143,083 - +0.2 6,143,083 - -3.6 6,143,083 - +0.5 6,143,442 + AO356_26975 0.72 +1.4 6,143,443 - AO356_26975 0.72 -1.8 6,143,479 - AO356_26975 0.79 +0.1 6,143,496 + AO356_26975 0.82 -1.2 6,143,496 + AO356_26975 0.82 +0.1 6,143,497 - AO356_26975 0.82 +2.2 6,143,717 - -0.8 6,143,717 - -1.9 6,143,717 - -0.9 6,143,717 - -1.8 6,143,722 + -0.9 6,143,854 + AO356_26980 0.48 +0.1 6,143,866 + AO356_26980 0.53 -1.8 6,143,866 + AO356_26980 0.53 -0.1 6,143,867 - AO356_26980 0.53 -0.9 6,143,894 - AO356_26980 0.65 +0.1 6,143,903 + AO356_26980 0.69 +0.7 6,143,904 - AO356_26980 0.69 +0.4 6,143,904 - AO356_26980 0.69 +0.3 6,143,904 - AO356_26980 0.69 +0.6 6,143,904 - AO356_26980 0.69 +1.9 6,143,972 + +0.3
Or see this region's nucleotide sequence