Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25560 and AO356_25565 are separated by 14 nucleotides AO356_25565 and AO356_25570 are separated by 12 nucleotides AO356_25570 and AO356_25575 are separated by 52 nucleotides AO356_25575 and AO356_25580 are separated by 84 nucleotides
AO356_25560: AO356_25560 - 2-keto-3-deoxy-L-rhamnonate aldolase, at 5,778,390 to 5,779,187
_25560
AO356_25565: AO356_25565 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 5,779,202 to 5,780,005
_25565
AO356_25570: AO356_25570 - MFS transporter, at 5,780,018 to 5,781,322
_25570
AO356_25575: AO356_25575 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 5,781,375 to 5,781,767
_25575
AO356_25580: AO356_25580 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 5,781,852 to 5,782,709
_25580
Position (kb) 5780
5781
5782 Strain fitness (log2 ratio) -1
0
1
2 at 5779.102 kb on + strand, within AO356_25560 at 5779.102 kb on + strand, within AO356_25560 at 5779.103 kb on - strand, within AO356_25560 at 5779.103 kb on - strand, within AO356_25560 at 5779.767 kb on + strand, within AO356_25565 at 5779.888 kb on - strand, within AO356_25565 at 5780.020 kb on - strand at 5780.090 kb on + strand at 5780.091 kb on - strand at 5780.095 kb on - strand at 5780.460 kb on - strand, within AO356_25570 at 5781.592 kb on + strand, within AO356_25575 at 5781.746 kb on - strand at 5781.804 kb on + strand at 5781.805 kb on - strand at 5782.313 kb on - strand, within AO356_25580 at 5782.313 kb on - strand, within AO356_25580
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,779,102 + AO356_25560 0.89 +1.0 5,779,102 + AO356_25560 0.89 -1.1 5,779,103 - AO356_25560 0.89 -0.4 5,779,103 - AO356_25560 0.89 -0.2 5,779,767 + AO356_25565 0.70 +1.5 5,779,888 - AO356_25565 0.85 +0.5 5,780,020 - +0.9 5,780,090 + -1.0 5,780,091 - +1.4 5,780,095 - +1.1 5,780,460 - AO356_25570 0.34 -1.0 5,781,592 + AO356_25575 0.55 +0.8 5,781,746 - +1.2 5,781,804 + -0.9 5,781,805 - +0.0 5,782,313 - AO356_25580 0.54 +0.8 5,782,313 - AO356_25580 0.54 +2.6
Or see this region's nucleotide sequence