Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24890 and AO356_24895 are separated by 51 nucleotides AO356_24895 and AO356_24900 are separated by 66 nucleotides AO356_24900 and AO356_24905 are separated by 233 nucleotides
AO356_24890: AO356_24890 - acetoin dehydrogenase, at 5,640,514 to 5,641,626
_24890
AO356_24895: AO356_24895 - butanediol dehydrogenase, at 5,641,678 to 5,642,772
_24895
AO356_24900: AO356_24900 - damage-inducible protein DinB, at 5,642,839 to 5,643,393
_24900
AO356_24905: AO356_24905 - 4-hydroxy-tetrahydrodipicolinate synthase, at 5,643,627 to 5,644,544
_24905
Position (kb) 5641
5642
5643 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5641.181 kb on + strand, within AO356_24890 at 5641.237 kb on + strand, within AO356_24890 at 5641.351 kb on + strand, within AO356_24890 at 5641.352 kb on - strand, within AO356_24890 at 5641.461 kb on - strand, within AO356_24890 at 5641.677 kb on + strand at 5641.677 kb on + strand at 5641.678 kb on - strand at 5641.678 kb on - strand at 5641.840 kb on + strand, within AO356_24895 at 5641.841 kb on - strand, within AO356_24895 at 5642.020 kb on + strand, within AO356_24895 at 5642.020 kb on + strand, within AO356_24895 at 5642.021 kb on - strand, within AO356_24895 at 5642.042 kb on - strand, within AO356_24895 at 5642.128 kb on + strand, within AO356_24895 at 5642.128 kb on + strand, within AO356_24895 at 5642.129 kb on - strand, within AO356_24895 at 5642.599 kb on + strand, within AO356_24895 at 5642.913 kb on + strand, within AO356_24900 at 5642.913 kb on + strand, within AO356_24900 at 5642.914 kb on - strand, within AO356_24900 at 5643.194 kb on - strand, within AO356_24900 at 5643.194 kb on - strand, within AO356_24900 at 5643.230 kb on + strand, within AO356_24900 at 5643.230 kb on + strand, within AO356_24900 at 5643.231 kb on - strand, within AO356_24900 at 5643.386 kb on + strand at 5643.386 kb on + strand at 5643.387 kb on - strand at 5643.387 kb on - strand at 5643.691 kb on + strand at 5643.691 kb on + strand at 5643.692 kb on - strand at 5643.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,641,181 + AO356_24890 0.60 -0.7 5,641,237 + AO356_24890 0.65 +0.1 5,641,351 + AO356_24890 0.75 -1.0 5,641,352 - AO356_24890 0.75 -1.4 5,641,461 - AO356_24890 0.85 +0.8 5,641,677 + +1.4 5,641,677 + -0.1 5,641,678 - +1.2 5,641,678 - +1.9 5,641,840 + AO356_24895 0.15 +0.1 5,641,841 - AO356_24895 0.15 -0.9 5,642,020 + AO356_24895 0.31 -0.5 5,642,020 + AO356_24895 0.31 +1.8 5,642,021 - AO356_24895 0.31 -0.6 5,642,042 - AO356_24895 0.33 +0.2 5,642,128 + AO356_24895 0.41 +0.9 5,642,128 + AO356_24895 0.41 +0.8 5,642,129 - AO356_24895 0.41 -0.8 5,642,599 + AO356_24895 0.84 -0.8 5,642,913 + AO356_24900 0.13 +0.4 5,642,913 + AO356_24900 0.13 +1.3 5,642,914 - AO356_24900 0.14 -2.7 5,643,194 - AO356_24900 0.64 +1.1 5,643,194 - AO356_24900 0.64 -0.7 5,643,230 + AO356_24900 0.70 +2.2 5,643,230 + AO356_24900 0.70 -2.8 5,643,231 - AO356_24900 0.71 +1.5 5,643,386 + -0.5 5,643,386 + -0.6 5,643,387 - +0.9 5,643,387 - +0.9 5,643,691 + +0.2 5,643,691 + +0.4 5,643,692 - -0.5 5,643,692 - +1.0
Or see this region's nucleotide sequence