Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24005 and AO356_24010 overlap by 4 nucleotides AO356_24010 and AO356_24015 overlap by 11 nucleotides AO356_24015 and AO356_24020 overlap by 14 nucleotides AO356_24020 and AO356_24025 are separated by 13 nucleotides
AO356_24005: AO356_24005 - aspartate aminotransferase, at 5,382,028 to 5,383,239
_24005
AO356_24010: AO356_24010 - amino acid ABC transporter, at 5,383,236 to 5,383,970
_24010
AO356_24015: AO356_24015 - amino acid ABC transporter, at 5,383,960 to 5,384,748
_24015
AO356_24020: AO356_24020 - phosphate ABC transporter ATP-binding protein, at 5,384,735 to 5,385,571
_24020
AO356_24025: AO356_24025 - cysteine dioxygenase, at 5,385,585 to 5,386,184
_24025
Position (kb) 5383
5384
5385 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5383.129 kb on + strand at 5383.130 kb on - strand at 5383.130 kb on - strand at 5383.290 kb on + strand at 5383.311 kb on + strand, within AO356_24010 at 5383.498 kb on - strand, within AO356_24010 at 5383.498 kb on - strand, within AO356_24010 at 5383.821 kb on + strand, within AO356_24010 at 5383.821 kb on + strand, within AO356_24010 at 5383.822 kb on - strand, within AO356_24010 at 5383.929 kb on + strand at 5384.056 kb on + strand, within AO356_24015 at 5384.056 kb on + strand, within AO356_24015 at 5384.056 kb on + strand, within AO356_24015 at 5384.056 kb on + strand, within AO356_24015 at 5384.057 kb on - strand, within AO356_24015 at 5384.057 kb on - strand, within AO356_24015 at 5384.057 kb on - strand, within AO356_24015 at 5384.282 kb on - strand, within AO356_24015 at 5384.289 kb on + strand, within AO356_24015 at 5384.289 kb on + strand, within AO356_24015 at 5384.289 kb on + strand, within AO356_24015 at 5384.290 kb on - strand, within AO356_24015 at 5384.290 kb on - strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.293 kb on + strand, within AO356_24015 at 5384.294 kb on - strand, within AO356_24015 at 5384.294 kb on - strand, within AO356_24015 at 5384.294 kb on - strand, within AO356_24015 at 5384.294 kb on - strand, within AO356_24015 at 5384.294 kb on - strand, within AO356_24015 at 5384.329 kb on + strand, within AO356_24015 at 5384.329 kb on + strand, within AO356_24015 at 5384.330 kb on - strand, within AO356_24015 at 5384.659 kb on + strand, within AO356_24015 at 5384.659 kb on + strand, within AO356_24015 at 5384.689 kb on + strand at 5384.719 kb on + strand at 5384.735 kb on - strand at 5385.026 kb on + strand, within AO356_24020 at 5385.026 kb on + strand, within AO356_24020 at 5385.026 kb on + strand, within AO356_24020 at 5385.027 kb on - strand, within AO356_24020 at 5385.027 kb on - strand, within AO356_24020 at 5385.027 kb on - strand, within AO356_24020 at 5385.027 kb on - strand, within AO356_24020 at 5385.389 kb on + strand, within AO356_24020 at 5385.390 kb on - strand, within AO356_24020 at 5385.555 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,383,129 + +1.7 5,383,130 - -0.2 5,383,130 - -1.2 5,383,290 + +0.4 5,383,311 + AO356_24010 0.10 -2.0 5,383,498 - AO356_24010 0.36 -0.4 5,383,498 - AO356_24010 0.36 -0.5 5,383,821 + AO356_24010 0.80 -3.3 5,383,821 + AO356_24010 0.80 +0.1 5,383,822 - AO356_24010 0.80 +0.7 5,383,929 + +0.7 5,384,056 + AO356_24015 0.12 +1.1 5,384,056 + AO356_24015 0.12 -0.0 5,384,056 + AO356_24015 0.12 +0.8 5,384,056 + AO356_24015 0.12 +3.4 5,384,057 - AO356_24015 0.12 +0.7 5,384,057 - AO356_24015 0.12 +1.8 5,384,057 - AO356_24015 0.12 -1.4 5,384,282 - AO356_24015 0.41 +2.4 5,384,289 + AO356_24015 0.42 -1.7 5,384,289 + AO356_24015 0.42 -0.2 5,384,289 + AO356_24015 0.42 +1.3 5,384,290 - AO356_24015 0.42 -0.2 5,384,290 - AO356_24015 0.42 +1.3 5,384,293 + AO356_24015 0.42 +0.1 5,384,293 + AO356_24015 0.42 +1.8 5,384,293 + AO356_24015 0.42 -1.1 5,384,293 + AO356_24015 0.42 +0.7 5,384,293 + AO356_24015 0.42 +0.9 5,384,293 + AO356_24015 0.42 +0.1 5,384,293 + AO356_24015 0.42 +1.1 5,384,293 + AO356_24015 0.42 +1.3 5,384,293 + AO356_24015 0.42 +0.2 5,384,294 - AO356_24015 0.42 +0.5 5,384,294 - AO356_24015 0.42 +1.7 5,384,294 - AO356_24015 0.42 -1.7 5,384,294 - AO356_24015 0.42 +1.6 5,384,294 - AO356_24015 0.42 +1.6 5,384,329 + AO356_24015 0.47 -1.5 5,384,329 + AO356_24015 0.47 -0.3 5,384,330 - AO356_24015 0.47 -0.1 5,384,659 + AO356_24015 0.89 -1.5 5,384,659 + AO356_24015 0.89 -0.5 5,384,689 + -1.0 5,384,719 + +0.8 5,384,735 - +0.6 5,385,026 + AO356_24020 0.35 +2.4 5,385,026 + AO356_24020 0.35 -3.1 5,385,026 + AO356_24020 0.35 -1.0 5,385,027 - AO356_24020 0.35 -1.4 5,385,027 - AO356_24020 0.35 +0.4 5,385,027 - AO356_24020 0.35 +1.6 5,385,027 - AO356_24020 0.35 -0.4 5,385,389 + AO356_24020 0.78 -1.3 5,385,390 - AO356_24020 0.78 -0.2 5,385,555 - -0.1
Or see this region's nucleotide sequence