Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23815 and AO356_23820 are separated by 245 nucleotides AO356_23820 and AO356_23825 overlap by 11 nucleotides
AO356_23815: AO356_23815 - amidase, at 5,341,381 to 5,343,087
_23815
AO356_23820: AO356_23820 - thermostable hemolysin, at 5,343,333 to 5,344,010
_23820
AO356_23825: AO356_23825 - long-chain acyl-CoA synthetase, at 5,344,000 to 5,345,481
_23825
Position (kb) 5343
5344
5345 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5342.643 kb on - strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.663 kb on + strand, within AO356_23815 at 5342.664 kb on - strand, within AO356_23815 at 5342.828 kb on + strand, within AO356_23815 at 5342.829 kb on - strand, within AO356_23815 at 5342.829 kb on - strand, within AO356_23815 at 5342.847 kb on - strand, within AO356_23815 at 5342.847 kb on - strand, within AO356_23815 at 5342.978 kb on + strand at 5342.979 kb on - strand at 5342.999 kb on + strand at 5343.163 kb on - strand at 5343.163 kb on - strand at 5343.195 kb on + strand at 5343.196 kb on - strand at 5343.196 kb on - strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.238 kb on + strand at 5343.239 kb on - strand at 5343.239 kb on - strand at 5343.252 kb on - strand at 5343.296 kb on + strand at 5343.297 kb on - strand at 5343.297 kb on - strand at 5343.310 kb on - strand at 5343.598 kb on - strand, within AO356_23820 at 5343.598 kb on - strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.732 kb on + strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.733 kb on - strand, within AO356_23820 at 5343.885 kb on + strand, within AO356_23820 at 5343.885 kb on + strand, within AO356_23820 at 5343.886 kb on - strand, within AO356_23820 at 5344.065 kb on + strand at 5344.066 kb on - strand at 5344.111 kb on + strand at 5344.603 kb on + strand, within AO356_23825 at 5344.604 kb on - strand, within AO356_23825 at 5344.604 kb on - strand, within AO356_23825 at 5344.604 kb on - strand, within AO356_23825
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,342,643 - AO356_23815 0.74 +2.4 5,342,663 + AO356_23815 0.75 -1.8 5,342,663 + AO356_23815 0.75 +1.1 5,342,663 + AO356_23815 0.75 -0.4 5,342,664 - AO356_23815 0.75 -1.4 5,342,828 + AO356_23815 0.85 -1.4 5,342,829 - AO356_23815 0.85 -0.4 5,342,829 - AO356_23815 0.85 +1.3 5,342,847 - AO356_23815 0.86 -0.4 5,342,847 - AO356_23815 0.86 +1.9 5,342,978 + -2.1 5,342,979 - -0.5 5,342,999 + +0.1 5,343,163 - +0.2 5,343,163 - -0.8 5,343,195 + +0.3 5,343,196 - -2.0 5,343,196 - +0.5 5,343,238 + +0.9 5,343,238 + -1.4 5,343,238 + +1.5 5,343,238 + -0.8 5,343,238 + +0.6 5,343,238 + +1.6 5,343,239 - -2.2 5,343,239 - +0.8 5,343,252 - -1.7 5,343,296 + +1.8 5,343,297 - +0.2 5,343,297 - +1.4 5,343,310 - -0.8 5,343,598 - AO356_23820 0.39 +2.6 5,343,598 - AO356_23820 0.39 +0.8 5,343,732 + AO356_23820 0.59 +0.3 5,343,732 + AO356_23820 0.59 -0.3 5,343,732 + AO356_23820 0.59 -1.0 5,343,733 - AO356_23820 0.59 +1.3 5,343,733 - AO356_23820 0.59 -1.7 5,343,733 - AO356_23820 0.59 +0.3 5,343,733 - AO356_23820 0.59 +0.5 5,343,885 + AO356_23820 0.81 -2.2 5,343,885 + AO356_23820 0.81 -0.5 5,343,886 - AO356_23820 0.82 +1.1 5,344,065 + +1.6 5,344,066 - +2.1 5,344,111 + +2.1 5,344,603 + AO356_23825 0.41 -0.6 5,344,604 - AO356_23825 0.41 +0.3 5,344,604 - AO356_23825 0.41 -1.3 5,344,604 - AO356_23825 0.41 -0.3
Or see this region's nucleotide sequence