Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22970 and AO356_22975 are separated by 257 nucleotides AO356_22975 and AO356_22980 are separated by 3 nucleotides
AO356_22970: AO356_22970 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) <small>(from data)</small>, at 5,127,612 to 5,128,631
_22970
AO356_22975: AO356_22975 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) <small>(from data)</small>, at 5,128,889 to 5,130,274
_22975
AO356_22980: AO356_22980 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 5,130,278 to 5,131,546
_22980
Position (kb) 5128
5129
5130
5131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5128.007 kb on - strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.067 kb on - strand, within AO356_22970 at 5128.162 kb on + strand, within AO356_22970 at 5128.163 kb on - strand, within AO356_22970 at 5128.280 kb on - strand, within AO356_22970 at 5128.474 kb on + strand, within AO356_22970 at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.589 kb on - strand at 5128.641 kb on + strand at 5128.641 kb on + strand at 5128.642 kb on - strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.804 kb on + strand at 5128.805 kb on - strand at 5129.064 kb on - strand, within AO356_22975 at 5129.064 kb on - strand, within AO356_22975 at 5129.572 kb on + strand, within AO356_22975 at 5129.573 kb on - strand, within AO356_22975 at 5129.583 kb on + strand, within AO356_22975 at 5129.583 kb on + strand, within AO356_22975 at 5129.584 kb on - strand, within AO356_22975 at 5129.643 kb on + strand, within AO356_22975 at 5129.643 kb on + strand, within AO356_22975 at 5129.643 kb on + strand, within AO356_22975 at 5129.644 kb on - strand, within AO356_22975 at 5129.730 kb on + strand, within AO356_22975 at 5129.730 kb on + strand, within AO356_22975 at 5130.220 kb on - strand at 5130.274 kb on + strand at 5130.274 kb on + strand at 5130.274 kb on + strand at 5130.274 kb on + strand at 5130.275 kb on - strand at 5130.275 kb on - strand at 5130.275 kb on - strand at 5130.279 kb on + strand at 5130.279 kb on + strand at 5130.280 kb on - strand at 5130.280 kb on - strand at 5130.280 kb on - strand at 5130.885 kb on + strand, within AO356_22980 at 5130.885 kb on + strand, within AO356_22980 at 5130.886 kb on - strand, within AO356_22980 at 5131.032 kb on + strand, within AO356_22980
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,128,007 - AO356_22970 0.39 -0.0 5,128,066 + AO356_22970 0.45 +0.6 5,128,066 + AO356_22970 0.45 -0.8 5,128,067 - AO356_22970 0.45 +1.8 5,128,162 + AO356_22970 0.54 -1.4 5,128,163 - AO356_22970 0.54 -1.9 5,128,280 - AO356_22970 0.65 -2.4 5,128,474 + AO356_22970 0.85 -0.2 5,128,588 + +0.3 5,128,588 + +1.0 5,128,588 + +0.3 5,128,588 + -0.3 5,128,589 - +1.1 5,128,641 + -1.1 5,128,641 + -0.4 5,128,642 - -0.5 5,128,703 + -0.5 5,128,703 + +0.0 5,128,703 + -0.5 5,128,703 + +0.8 5,128,703 + -2.9 5,128,703 + +0.6 5,128,703 + -0.5 5,128,703 + -2.7 5,128,703 + +2.3 5,128,704 - -2.2 5,128,704 - -3.6 5,128,704 - +0.2 5,128,704 - +0.5 5,128,753 + -0.9 5,128,753 + -0.6 5,128,753 + +0.9 5,128,753 + +0.5 5,128,753 + -0.3 5,128,753 + +0.0 5,128,754 - +1.4 5,128,754 - -1.8 5,128,754 - +0.4 5,128,754 - +0.6 5,128,754 - -1.8 5,128,754 - +1.4 5,128,754 - +1.9 5,128,804 + -1.4 5,128,805 - -1.6 5,129,064 - AO356_22975 0.13 +1.1 5,129,064 - AO356_22975 0.13 -0.6 5,129,572 + AO356_22975 0.49 +1.7 5,129,573 - AO356_22975 0.49 -1.5 5,129,583 + AO356_22975 0.50 -1.0 5,129,583 + AO356_22975 0.50 -0.0 5,129,584 - AO356_22975 0.50 +0.1 5,129,643 + AO356_22975 0.54 +0.1 5,129,643 + AO356_22975 0.54 +0.4 5,129,643 + AO356_22975 0.54 +0.6 5,129,644 - AO356_22975 0.54 +0.3 5,129,730 + AO356_22975 0.61 -0.5 5,129,730 + AO356_22975 0.61 +0.5 5,130,220 - -1.3 5,130,274 + -1.2 5,130,274 + +1.3 5,130,274 + -2.7 5,130,274 + +0.5 5,130,275 - +1.1 5,130,275 - +0.7 5,130,275 - +0.2 5,130,279 + +0.8 5,130,279 + +1.2 5,130,280 - +2.1 5,130,280 - -1.3 5,130,280 - +0.2 5,130,885 + AO356_22980 0.48 +0.1 5,130,885 + AO356_22980 0.48 +0.1 5,130,886 - AO356_22980 0.48 +1.0 5,131,032 + AO356_22980 0.59 +0.4
Or see this region's nucleotide sequence