Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22965 and AO356_22970 are separated by 83 nucleotides AO356_22970 and AO356_22975 are separated by 257 nucleotides
AO356_22965: AO356_22965 - hypothetical protein, at 5,126,599 to 5,127,528
_22965
AO356_22970: AO356_22970 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) (from data) , at 5,127,612 to 5,128,631
_22970
AO356_22975: AO356_22975 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) , at 5,128,889 to 5,130,274
_22975
Position (kb)
5127
5128
5129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5126.880 kb on + strand, within AO356_22965 at 5126.913 kb on + strand, within AO356_22965 at 5127.123 kb on - strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.169 kb on + strand, within AO356_22965 at 5127.170 kb on - strand, within AO356_22965 at 5127.328 kb on + strand, within AO356_22965 at 5127.328 kb on + strand, within AO356_22965 at 5127.329 kb on - strand, within AO356_22965 at 5127.329 kb on - strand, within AO356_22965 at 5127.419 kb on + strand, within AO356_22965 at 5127.426 kb on + strand, within AO356_22965 at 5127.427 kb on - strand, within AO356_22965 at 5127.542 kb on + strand at 5127.608 kb on + strand at 5127.609 kb on - strand at 5127.679 kb on + strand at 5127.679 kb on + strand at 5127.679 kb on + strand at 5127.680 kb on - strand at 5127.680 kb on - strand at 5127.742 kb on + strand, within AO356_22970 at 5127.742 kb on + strand, within AO356_22970 at 5127.742 kb on + strand, within AO356_22970 at 5127.743 kb on - strand, within AO356_22970 at 5128.007 kb on - strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.066 kb on + strand, within AO356_22970 at 5128.067 kb on - strand, within AO356_22970 at 5128.162 kb on + strand, within AO356_22970 at 5128.163 kb on - strand, within AO356_22970 at 5128.280 kb on - strand, within AO356_22970 at 5128.474 kb on + strand, within AO356_22970 at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.588 kb on + strand at 5128.589 kb on - strand at 5128.641 kb on + strand at 5128.641 kb on + strand at 5128.642 kb on - strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.703 kb on + strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.704 kb on - strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.753 kb on + strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.754 kb on - strand at 5128.804 kb on + strand at 5128.805 kb on - strand at 5129.064 kb on - strand, within AO356_22975 at 5129.064 kb on - strand, within AO356_22975 at 5129.572 kb on + strand, within AO356_22975 at 5129.573 kb on - strand, within AO356_22975 at 5129.583 kb on + strand, within AO356_22975 at 5129.583 kb on + strand, within AO356_22975 at 5129.584 kb on - strand, within AO356_22975
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 5,126,880 + AO356_22965 0.30 -3.3 5,126,913 + AO356_22965 0.34 +0.6 5,127,123 - AO356_22965 0.56 +0.9 5,127,169 + AO356_22965 0.61 +0.5 5,127,169 + AO356_22965 0.61 -1.0 5,127,169 + AO356_22965 0.61 +1.0 5,127,169 + AO356_22965 0.61 -2.5 5,127,169 + AO356_22965 0.61 +0.2 5,127,169 + AO356_22965 0.61 -1.7 5,127,170 - AO356_22965 0.61 -0.2 5,127,328 + AO356_22965 0.78 +0.3 5,127,328 + AO356_22965 0.78 +0.2 5,127,329 - AO356_22965 0.78 -0.5 5,127,329 - AO356_22965 0.78 -2.2 5,127,419 + AO356_22965 0.88 +0.2 5,127,426 + AO356_22965 0.89 -0.6 5,127,427 - AO356_22965 0.89 +0.6 5,127,542 + +0.8 5,127,608 + -0.2 5,127,609 - -0.1 5,127,679 + -0.8 5,127,679 + +0.7 5,127,679 + -2.1 5,127,680 - +1.0 5,127,680 - +0.8 5,127,742 + AO356_22970 0.13 -1.6 5,127,742 + AO356_22970 0.13 +1.5 5,127,742 + AO356_22970 0.13 -0.5 5,127,743 - AO356_22970 0.13 -0.5 5,128,007 - AO356_22970 0.39 -0.0 5,128,066 + AO356_22970 0.45 +0.6 5,128,066 + AO356_22970 0.45 -0.8 5,128,067 - AO356_22970 0.45 +1.8 5,128,162 + AO356_22970 0.54 -1.4 5,128,163 - AO356_22970 0.54 -1.9 5,128,280 - AO356_22970 0.65 -2.4 5,128,474 + AO356_22970 0.85 -0.2 5,128,588 + +0.3 5,128,588 + +1.0 5,128,588 + +0.3 5,128,588 + -0.3 5,128,589 - +1.1 5,128,641 + -1.1 5,128,641 + -0.4 5,128,642 - -0.5 5,128,703 + -0.5 5,128,703 + +0.0 5,128,703 + -0.5 5,128,703 + +0.8 5,128,703 + -2.9 5,128,703 + +0.6 5,128,703 + -0.5 5,128,703 + -2.7 5,128,703 + +2.3 5,128,704 - -2.2 5,128,704 - -3.6 5,128,704 - +0.2 5,128,704 - +0.5 5,128,753 + -0.9 5,128,753 + -0.6 5,128,753 + +0.9 5,128,753 + +0.5 5,128,753 + -0.3 5,128,753 + +0.0 5,128,754 - +1.4 5,128,754 - -1.8 5,128,754 - +0.4 5,128,754 - +0.6 5,128,754 - -1.8 5,128,754 - +1.4 5,128,754 - +1.9 5,128,804 + -1.4 5,128,805 - -1.6 5,129,064 - AO356_22975 0.13 +1.1 5,129,064 - AO356_22975 0.13 -0.6 5,129,572 + AO356_22975 0.49 +1.7 5,129,573 - AO356_22975 0.49 -1.5 5,129,583 + AO356_22975 0.50 -1.0 5,129,583 + AO356_22975 0.50 -0.0 5,129,584 - AO356_22975 0.50 +0.1
Or see this region's nucleotide sequence